Clinical and Experimental Reproductive Medicine

  • 제38권4호
  • /
  • Pages.203-209
  • /
  • 2011
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지
DOI QR코드

DOI QR Code

$In$ $vitro$ development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification

  • Shin, Mi-Ra (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine) ;
  • Choi, Hye-Won (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine) ;
  • Kim, Myo-Kyung (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine) ;
  • Lee, Sun-Hee (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine) ;
  • Lee, Hyoung-Song (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine) ;
  • Lim, Chun-Kyu (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine)
  • 투고 : 2011.09.08
  • 심사 : 2011.11.01
  • 발행 : 2011.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. Methods: Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divided into three groups. One group was maintained as fresh controls (n=42), one was frozen by slow freezing (n=43), and one was cooled by vitrification (n=43). After thawing or cooling, survival rates, development to blastocyst, and cell numbers and inner cell mass (ICM) cell numbers of blastocysts were compared with those of the control group. The expressions of eight genes ($Rbm3$, $Birc5$, $Sod1$, $Sod2$, $Cirbp$, $Caspase3$, $Trp53$, $Hsp70.1$) were examined by real time-quantitative polymerase chain reaction in the fresh and frozen-thawed embryos. Results: There were no significant differences in the slow freezing and vitrification groups' survival rate after thawing (88.4% vs. 88.4%), development to blastocyst (100% vs. 97.4%), cell numbers ($107.0{\pm}21.0$ vs. $115.0{\pm}19.7$), or ICM cell numbers of blastocysts ($11.3{\pm}5.2$ vs. $11.1{\pm}3.7$). Cell numbers of blastocysts were significantly ($p$ <0.05) lower in the frozen-thawed embryos than the fresh embryos. There were no significant differences in the slow freezing and the vitrification groups' expressions of the eight genes. The expressions of $CirbP$ and $Hsp70.1$ were higher in the frozen-thawed embryos than in the fresh embryos but there were no significant differences. Conclusion: These results suggest that there were no significant differences between embryos that underwent slow freezing and vitrification.

키워드

참고문헌

  1. Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet 1978;2:366.
  2. Liebermann J, Dietl J, Vanderzwalmen P, Tucker MJ. Recent developments in human oocyte, embryo and blastocyst vitrification: where are we now? Reprod Biomed Online 2003;7:623-33. https://doi.org/10.1016/S1472-6483(10)62084-6
  3. Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 1983;305:707-9. https://doi.org/10.1038/305707a0
  4. Cohen J, DeVane GW, Elsner CW, Fehilly CB, Kort HI, Massey JB, et al. Cryopreservation of zygotes and early cleaved human embryos. Fertil Steril 1988;49:283-9. https://doi.org/10.1016/S0015-0282(16)59717-9
  5. Rama Raju GA, Haranath GB, Krishna KM, Prakash GJ, Madan K. Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates. Reprod Biomed Online 2005;11:434-7. https://doi.org/10.1016/S1472-6483(10)61135-2
  6. Bielanski A, Nadin-Davis S, Sapp T, Lutze-Wallace C. Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology 2000;40:110-6. https://doi.org/10.1006/cryo.1999.2227
  7. Balaban B, Urman B, Ata B, Isiklar A, Larman MG, Hamilton R, et al. A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation. Hum Reprod 2008;23:1976-82. https://doi.org/10.1093/humrep/den222
  8. Loutradi KE, Kolibianakis EM, Venetis CA, Papanikolaou EG, Pados G, Bontis I, et al. Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil Steril 2008;90:186-93. https://doi.org/10.1016/j.fertnstert.2007.06.010
  9. Kolibianakis EM, Venetis CA, Tarlatzis BC. Cryopreservation of human embryos by vitrification or slow freezing: which one is better? Curr Opin Obstet Gynecol 2009;21:270-4. https://doi.org/10.1097/GCO.0b013e3283297dd6
  10. Rezazadeh Valojerdi M, Eftekhari-Yazdi P, Karimian L, Hassani F, Movaghar B. Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos. J Assist Reprod Genet 2009;26:347-54. https://doi.org/10.1007/s10815-009-9318-6
  11. Lin TK, Su JT, Lee FK, Lin YR, Lo HC. Cryotop vitrification as compared to conventional slow freezing for human embryos at the cleavage stage: survival and outcomes. Taiwan J Obstet Gynecol 2010;49:272-8. https://doi.org/10.1016/S1028-4559(10)60060-5
  12. AbdelHafez FF, Desai N, Abou-Setta AM, Falcone T, Goldfarb J. Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis. Reprod Biomed Online 2010;20:209-22. https://doi.org/10.1016/j.rbmo.2009.11.013
  13. Uechi H, Tsutsumi O, Morita Y, Takai Y, Taketani Y. Comparison of the effects of controlled-rate cryopreservation and vitrification on 2-cell mouse embryos and their subsequent development. Hum Reprod 1999;14:2827-32. https://doi.org/10.1093/humrep/14.11.2827
  14. Naik BR, Rao BS, Vagdevi R, Gnanprakash M, Amarnath D, Rao VH. Conventional slow freezing, vitrification and open pulled straw (OPS) vitrification of rabbit embryos. Anim Reprod Sci 2005;86:329-38. https://doi.org/10.1016/j.anireprosci.2004.07.008
  15. Mamo S, Bodo S, Kobolak J, Polgar Z, Tolgyesi G, Dinnyes A. Gene expression profiles of vitrified in vivo derived 8-cell stage mouse embryos detected by high density oligonucleotide microarrays. Mol Reprod Dev 2006;73:1380-92. https://doi.org/10.1002/mrd.20588
  16. Boonkusol D, Gal AB, Bodo S, Gorhony B, Kitiyanant Y, Dinnyes A. Gene expression profiles and in vitro development following vitrification of pronuclear and 8-cell stage mouse embryos. Mol Reprod Dev 2006;73:700-8. https://doi.org/10.1002/mrd.20450
  17. Van der Elst J, Amerijckx Y, Van Steirteghem A. Ultra-rapid freezing of mouse oocytes lowers the cell number in the inner cell mass of 5 day old in-vitro cultured blastocysts. Hum Reprod 1998;13:1595-9. https://doi.org/10.1093/humrep/13.6.1595
  18. Selick CE, Hofmann GE, Albano C, Horowitz GM, Copperman AB, Garrisi GJ, et al. Embryo quality and pregnancy potential of fresh compared with frozen embryos-is freezing detrimental to high quality embryos? Hum Reprod 1995;10:392-5. https://doi.org/10.1093/oxfordjournals.humrep.a135950
  19. Uechi H, Tsutsumi O, Morita Y, Taketani Y. Cryopreservation of mouse embryos affects later embryonic development possibly through reduced expression of the glucose transporter GLUT1. Mol Reprod Dev 1997;48:496-500. https://doi.org/10.1002/(SICI)1098-2795(199712)48:4<496::AID-MRD10>3.0.CO;2-S
  20. Miyake T, Kasai M, Zhu SE, Sakurai T, Machida T. Vitrification of mouse oocytes and embryos at various stages of development in an ethylene glycol-based solution by a simple method. Theriogenology 1993;40:121-34. https://doi.org/10.1016/0093-691X(93)90346-7
  21. Arav A, Zeron Y, Ocheretny A. A new device and method for vitrification increases the cooling rate and allows successful cryopreservation of bovine oocytes. Theriogenology 2000;53:248-9.
  22. Hochi S, Akiyama M, Minagawa G, Kimura K, Hanada A. Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes. Cryobiology 2001;42:69-73. https://doi.org/10.1006/cryo.2001.2298
  23. Isachenko V, Alabart JL, Nawroth F, Isachenko E, Vajta G, Folch J. The open pulled straw vitrification of ovine GV-oocytes: positive effect of rapid cooling or rapid thawing or both? Cryo Letters 2001;22:157-62.
  24. Iwasaki S, Yoshiba N, Ushijima H, Watanabe S, Nakahara T. Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo. J Reprod Fertil 1990;90:279-84. https://doi.org/10.1530/jrf.0.0900279
  25. Loskutoff NM, Johnson WH, Betteridge KJ. The developmental competence of bovine embryos with reduced cell numbers. Theriogenology 1993;39:95-107. https://doi.org/10.1016/0093-691X(93)90026-2
  26. Tao T, Reichelt B, Niemann H. Ratio of inner cell mass and trophoblastic cells in demi- and intact pig embryos. J Reprod Fertil 1995;104:251-8. https://doi.org/10.1530/jrf.0.1040251
  27. Edwards RG. Causes of early embryonic loss in human pregnancy. Hum Reprod 1986;1:185-98. https://doi.org/10.1093/oxfordjournals.humrep.a136378

피인용 문헌

  1. Blastocyst transfer in frozen-thawed cycles vol.39, pp.3, 2012, https://doi.org/10.5653/cerm.2012.39.3.114
  2. Establishment of anin VitroModel of the Human Placental Barrier by Placenta Slice Culture and Ussing Chamber vol.77, pp.5, 2011, https://doi.org/10.1271/bbb.120976
  3. Clinical outcomes of vitrified-thawed embryo transfer using a pull and cut straw method vol.56, pp.3, 2013, https://doi.org/10.5468/ogs.2013.56.3.182
  4. Direct Comparison of the Effects of Slow Freezing and Vitrification on Late Blastocyst Gene Expression, Development, Implantation and Offspring of Rabbit Morulae vol.49, pp.3, 2011, https://doi.org/10.1111/rda.12320
  5. Cold-inducible proteins CIRP and RBM3, a unique couple with activities far beyond the cold vol.73, pp.20, 2011, https://doi.org/10.1007/s00018-016-2253-7
  6. Comparative efficacies of six different media for cryopreservation of immature buffalo (Bubalus bubalis) calf testis vol.28, pp.7, 2011, https://doi.org/10.1071/rd14171
  7. Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos vol.62, pp.2, 2016, https://doi.org/10.1262/jrd.2015-150
  8. Alternation of apoptotic and implanting genes expression of mouse embryos after re-vitrification vol.14, pp.8, 2016, https://doi.org/10.29252/ijrm.14.8.511
  9. Long Cut Straw Provides Stable the Rates of Survival, Pregnancy and Live Birth for Vitrification of Human Blasotcysts vol.20, pp.3, 2011, https://doi.org/10.12717/dr.2016.20.3.219
  10. Effects of Re-Vitrification of Mouse Morula and Early Blastocyst Stages on Apoptotic Gene Expression and Developmental Potential vol.19, pp.4, 2011, https://doi.org/10.22074/cellj.2018.4892
  11. Mild hypothermia promotes the viability of in vitro-produced bovine blastocysts and their transcriptional expression of the cold-inducible transcription factor Rbm3 during in vitro culture vol.65, pp.3, 2019, https://doi.org/10.1262/jrd.2018-142
  12. Effect of embryo cryopreservation on derivation efficiency, pluripotency, and differentiation capacity of mouse embryonic stem cells vol.234, pp.12, 2019, https://doi.org/10.1002/jcp.28759
  13. Vitrification yields higher cryo-survival rate than slow freezing in biopsied bovine in vitro produced blastocysts vol.171, pp.None, 2011, https://doi.org/10.1016/j.theriogenology.2021.04.020