Tuberculosis and Respiratory Diseases

  • 제71권1호
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  • Pages.8-14
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  • 2011
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  • 1738-3536(pISSN)
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  • 2005-6184(eISSN)
대한결핵및호흡기학회 (The Korean Academy of Tuberculosis and Respiratory Diseases)
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MicroRNA-23a: A Novel Serum Based Diagnostic Biomarker for Lung Adenocarcinoma

  • Lee, Yu-Mi (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Cho, Hyun-Jung (Department of Laboratory Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Lee, Soo-Young (Myunggok Research Institute for Medical Science, Konyang University Hospital, Konyang University College of Medicine) ;
  • Yun, Seong-Cheol (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Kim, Ji-Hye (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Lee, Shin-Yup (Department of Internal Medicine, Kyungpook National University Hospital, Kyungpook National University College of Medicine) ;
  • Kwon, Sun-Jung (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Choi, Eu-Gene (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Na, Moon-Jun (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine) ;
  • Kang, Jae-Ku (Department of Pharmacology, Konyang University College of Medicine) ;
  • Son, Ji-Woong (Department of Internal Medicine, Konyang University Hospital, Konyang University College of Medicine)
  • 투고 : 2011.03.22
  • 심사 : 2011.04.14
  • 발행 : 2011.07.30
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초록

Background: MicroRNAs (miRNAs) have demonstrated their potential as biomarkers for lung cancer diagnosis. In recent years, miRNAs have been found in body fluids such as serum, plasma, urine and saliva. Circulating miRNAs are highly stable and resistant to RNase activity along with, extreme pH and temperatures in serum and plasma. In this study, we investigated serum miRNA profiles that can be used as a diagnostic biomarker of non-small cell lung cancer (NSCLC). Methods: We compared the expression profile of miRNAs in the plasma of patients diagnosed with lung cancer using an miRNA microarray. The data from this assay were validated by quantitative real-time PCR (qRT-PCR). Results: Six miRNAs were overexpressed and three miRNAs were underexpressed in both tissue and serum from squamous cell carcinoma (SCC) patients. Sixteen miRNAs were overexpressed and twenty two miRNAs were underexpressed in both tissue and serum from adenocarcinoma (AC) patients. Of the four miRNAs chosen for qRT-PCR analysis, the expression of miR-23a was consistent with microarray results from AC patients. Receiver operating characteristic (ROC) curve analyses were done and revealed that the level of serum miR-23a was a potential marker for discriminating AC patients from chronic obstructive pulmonary disease (COPD) patients. Conclusion: Although a small number of patients were examined, the results from our study suggest that serum miR-23a can be used in the diagnosis of AC.

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참고문헌

  1. Ambros V. The functions of animal microRNAs. Nature 2004;431:350-5. https://doi.org/10.1038/nature02871
  2. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004;116:281-97. https://doi.org/10.1016/S0092-8674(04)00045-5
  3. Kim VN, Nam JW. Genomics of microRNA. Trends Genet 2006;22:165-73. https://doi.org/10.1016/j.tig.2006.01.003
  4. Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA 2006;103:2257-61. https://doi.org/10.1073/pnas.0510565103
  5. Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M, et al. Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell 2006;9:189-98. https://doi.org/10.1016/j.ccr.2006.01.025
  6. Son JW, Kim YJ, Cho HM, Lee SY, Jang JS, Choi JE, et al. MicroRNA expression profiles in Korean non-small cell lung cancer. Tuberc Respir Dis 2009;67:413-21. https://doi.org/10.4046/trd.2009.67.5.413
  7. Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 2005;120:15-20. https://doi.org/10.1016/j.cell.2004.12.035
  8. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA 2008;105:10513-8. https://doi.org/10.1073/pnas.0804549105
  9. Kroh EM, Parkin RK, Mitchell PS, Tewari M. Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRTPCR). Methods 2010;50:298-301. https://doi.org/10.1016/j.ymeth.2010.01.032
  10. Lodes MJ, Caraballo M, Suciu D, Munro S, Kumar A, Anderson B. Detection of cancer with serum miRNAs on an oligonucleotide microarray. PLoS One 2009;4:e6229. https://doi.org/10.1371/journal.pone.0006229
  11. Lawrie CH, Gal S, Dunlop HM, Pushkaran B, Liggins AP, Pulford K, et al. Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol 2008;141:672-5. https://doi.org/10.1111/j.1365-2141.2008.07077.x
  12. Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, et al. Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis. Genome Res 2006;16:1289-98. https://doi.org/10.1101/gr.5159906
  13. Rosenfeld N, Aharonov R, Meiri E, Rosenwald S, Spector Y, Zepeniuk M, et al. MicroRNAs accurately identify cancer tissue origin. Nat Biotechnol 2008;26:462-9. https://doi.org/10.1038/nbt1392
  14. Okies JE, Dietl C, Garrison HB, Starr A. Early and late results of resection of ventricular aneurysm. J Thorac Cardiovasc Surg 1978;75:255-60.
  15. Lebanony D, Benjamin H, Gilad S, Ezagouri M, Dov A, Ashkenazi K, et al. Diagnostic assay based on hsa-miR-205 expression distinguishes squamous from nonsquamous non-small-cell lung carcinoma. J Clin Oncol 2009;27:2030-7. https://doi.org/10.1200/JCO.2008.19.4134
  16. Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, et al. Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res 2004;64:3753-6. https://doi.org/10.1158/0008-5472.CAN-04-0637
  17. Saito M, Schetter AJ, Mollerup S, Kohno T, Skaug V, Bowman ED, et al. The association of microRNA expression with prognosis and progression in early-stage, non-small cell lung adenocarcinoma: a retrospective analysis of three cohorts. Clin Cancer Res 2011;17:1875-82. https://doi.org/10.1158/1078-0432.CCR-10-2961
  18. Cho WC, Chow AS, Au JS. Restoration of tumour suppressor hsa-miR-145 inhibits cancer cell growth in lung adenocarcinoma patients with epidermal growth factor receptor mutation. Eur J Cancer 2009;45:2197-206. https://doi.org/10.1016/j.ejca.2009.04.039
  19. Garofalo M, Quintavalle C, Di Leva G, Zanca C, Romano G, Taccioli C, et al. MicroRNA signatures of TRAIL resistance in human non-small cell lung cancer. Oncogene 2008;27:3845-55. https://doi.org/10.1038/onc.2008.6
  20. Mattie MD, Benz CC, Bowers J, Sensinger K, Wong L, Scott GK, et al. Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies. Mol Cancer 2006;5:24. https://doi.org/10.1186/1476-4598-5-24
  21. Zen K, Zhang CY. Circulating microRNAs: a novel class of biomarkers to diagnose and monitor human cancers. Med Res Rev 2010 Nov 9 [Epub]. DOI: 10.1002/med.20215.
  22. Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res 2008;18:997-1006. https://doi.org/10.1038/cr.2008.282
  23. Katoh T, Sakaguchi Y, Miyauchi K, Suzuki T, Kashiwabara S, Baba T, et al. Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2. Genes Dev 2009;23:433-8. https://doi.org/10.1101/gad.1761509
  24. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. Export of microRNAs and microRNA-protective protein by mammalian cells. Nucleic Acids Res 2010;38:7248-59. https://doi.org/10.1093/nar/gkq601
  25. Wang JF, Yu ML, Yu G, Bian JJ, Deng XM, Wan XJ, et al. Serum miR-146a and miR-223 as potential new biomarkers for sepsis. Biochem Biophys Res Commun 2010;394:184-8. https://doi.org/10.1016/j.bbrc.2010.02.145
  26. Xu J, Wu C, Che X, Wang L, Yu D, Zhang T, et al. Circulating microRNAs, miR-21, miR-122, and miR-223, in patients with hepatocellular carcinoma or chronic hepatitis. Mol Carcinog 2011;50:136-42. https://doi.org/10.1002/mc.20712
  27. Scapoli L, Palmieri A, Lo Muzio L, Pezzetti F, Rubini C, Girardi A, et al. MicroRNA expression profiling of oral carcinoma identifies new markers of tumor progression. Int J Immunopathol Pharmacol 2010;23:1229-34. https://doi.org/10.1177/039463201002300427
  28. Vaksman O, Stavnes HT, Kaern J, Trope CG, Davidson B, Reich R. miRNA profiling along tumour progression in ovarian carcinoma. J Cell Mol Med 2011;15:1593-602. https://doi.org/10.1111/j.1582-4934.2010.01148.x
  29. Gottardo F, Liu CG, Ferracin M, Calin GA, Fassan M, Bassi P, et al. Micro-RNA profiling in kidney and bladder cancers. Urol Oncol 2007;25:387-92. https://doi.org/10.1016/j.urolonc.2007.01.019
  30. Zhu LH, Liu T, Tang H, Tian RQ, Su C, Liu M, et al. MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor. FEBS J 2010;277:3726-34. https://doi.org/10.1111/j.1742-4658.2010.07773.x
  31. Huang S, He X, Ding J, Liang L, Zhao Y, Zhang Z, et al. Upregulation of miR-23a approximately 27a approximately 24 decreases transforming growth factor-beta-induced tumor-suppressive activities in human hepatocellular carcinoma cells. Int J Cancer 2008;123:972-8. https://doi.org/10.1002/ijc.23580

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