Tuberculosis and Respiratory Diseases

  • 제70권4호
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  • Pages.315-322
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  • 2011
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  • 1738-3536(pISSN)
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  • 2005-6184(eISSN)
대한결핵및호흡기학회 (The Korean Academy of Tuberculosis and Respiratory Diseases)
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폐암환자의 객담 및 암 조직에서 MAGE A 1-6와 SSX 1-9 유전자의 발현

Expression of MAGE A 1-6 and SSX 1-9 Genes in the Sputum and Cancer Tissue of the Lung Cancer Patients

  • 이연재 (이연재맥의원) ;
  • 이장훈 (영남대학교 의과대학 흉부외과학교실) ;
  • 이정철 (영남대학교 의과대학 흉부외과학교실) ;
  • 이관호 (영남대학교 의과대학 내과학교실)
  • Lee, Yeun-Jae (Leeyeunjae Meck Clinic) ;
  • Lee, Jang-Hoon (Department of Thoracic and Cardiovascular Surgery, Yeungnam University College of Medicine) ;
  • Lee, Jung-Cheul (Department of Thoracic and Cardiovascular Surgery, Yeungnam University College of Medicine) ;
  • Lee, Kwan-Ho (Department of Internal Medicine, Yeungnam University College of Medicine)
  • 투고 : 2011.01.05
  • 심사 : 2011.04.04
  • 발행 : 2011.04.30
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초록

Background: A variety of diagnostic modalities for lung cancer have been developed. To achieve efficient and early detection of lung cancer, we tried to measure the expression rates of the melanoma associated gene (MAGE) and synovial sarcoma on X chromosome (SSX) genes. Methods: We designed primers for the SSX gene. In addition to the pre-developed MAGE A primer, using an SSX gene primer was attempted to increase the detection rate. We obtained cancer tissues and cancer-free lung tissues from resected lung, sputum from lung cancer patients who had not undergone surgery, and sputum from healthy people and patients with benign intrathoracic diseases. Results: The sensitivity of the MAGE or SSX gene RT-PCR to identifying cancer tissue of the 69 lung cancer patients was 95.2% for squamous cell carcinoma (scc), 87.0% for adenocarcinoma, and 100% for small cell carcinoma. The mean sensitivity value was 94.2% (p=0.001). For adenocarcinoma, the additional use of the SSX gene resulted in a higher expression rate than MAGE alone (87% vs. 69.6%). The expression rate for the cancer-free lung tissue was 14.3% in scc, 17.4% in adenocarcinoma, and 25.0% in small cell carcinoma. In the induced sputum of 49 lung cancer patients who had not undergone surgery, the expression rate for one of the two genes was 65.5%. The expression rate for the sputum of healthy people and benign intrathoracic diseases by MAGE or SSX gene reverse transcription polymerase chain reaction (RT-PCR) was 3.8% and 17.7%. Conclusion: Detecting lung cancer using the expression of MAGE and SSX genes in lung cancer tissue has high sensitivity.

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피인용 문헌

  1. 주변부 폐암에서 기관지세척액을 이용한 MAGE유전자검사법의 임상적 유용성 vol.72, pp.2, 2011, https://doi.org/10.4046/trd.2012.72.2.156