Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea

국내 가축 유래 대장균에서 CTX-M 및 TEM형 extended-spectrum β-lactamases의 검출

  • Cho, Jae-Keun (Metropolitan Health & Enviornmental Research Institute) ;
  • Sung, Myung-Suk (Gyeongbuk Veterinary Service Laboratory) ;
  • Kim, Jin-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University)
  • 조재근 (대구광역시 보건환경연구원) ;
  • 성명숙 (경상북도 가축위생시험소) ;
  • 김진현 (경북대학교 수의과대학) ;
  • 김기석 (경북대학교 수의과대학)
  • Received : 2011.02.25
  • Accepted : 2011.03.24
  • Published : 2011.03.30
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Abstract

This study was conducted to investigate the prevalence and genotypes of extended-spectrum ${\beta}$-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3~4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both $bla_{CTX-15}$ and $bla_{TEM-1}$, and one isolate from chicken have $bla_{CTX-15}$ and $bla_{TEM-116}$. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that $bla_{TEM-116}$ gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.

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