DOI QR코드

DOI QR Code

Occurrence and Decontamination of Mycotoxins in Swine Feed

  • Chaytor, Alexandra C. (North Carolina State University) ;
  • Hansen, Jeff A. (North Carolina State University) ;
  • Van Heugten, Eric (North Carolina State University) ;
  • See, M. Todd (North Carolina State University) ;
  • Kim, Sung-Woo (North Carolina State University)
  • Received : 2010.10.06
  • Accepted : 2010.11.04
  • Published : 2011.05.01

Abstract

Contamination of agricultural crops by mycotoxins results in significant economic losses for grain producers and, when consumed, it can cause reduced growth and health in a wide range of animal species. Hundreds of mycotoxin producing molds exist, however each has a different frequency and pattern of occurrence, as well as differences in the severity of the diseases (mycotoxicoses) they cause. Among the mycotoxins considered to be major contaminates are aflatoxin, deoxynivalenol, fumonisin, ochratoxin, and zearalenone. Although a multitude of species can be harmed by consumption of these mycotoxins, swine appear to be the most commonly affected commodity species. The swine industry can thus experience great losses due to the presence of mycotoxin contamination in feeds. Subsequently, recognition and prevention of mycotoxicoses is extremely important and dependent on adequate grain sampling and analysis methods pre-harvest, as well as effective strategies post-harvest to reduce consumption by animals. The aim of this review is to provide an overview of the major mycotoxin contaminants in grains, to describe methods of analysis and prevention to reduce mycotoxicoses in swine and other animals, and finally to discuss how mycotoxins directly affect swine production.

Keywords

References

  1. Accensi, F., P. Pinton, P. Callu, N. Abella-Bourges, J. -F. Guelfi, F. Grosjean and I. P. Oswald. 2006. Ingestion of low doses of deoxynivalenol does not affect hematological, biochemical, or immune responses of piglets. J. Anim. Sci. 84:1935-1942. https://doi.org/10.2527/jas.2005-355
  2. Agag, B. I. 2004. Mycotoxins in foods and feeds: aflatoxins. Ass. Univ. Bull. Environ. Res. 7:173-205.
  3. Anderson, D., J. D. Anderson and J. Sawyer. 2008. Impact of the ethanol boom on livestock and dairy industries: what are they going to eat? J. Agric. Appl. Econ. 40:573-579.
  4. Bacon, C. W., J. K. Porter, W. P. Norred and J. F. Leslie. 1996. Production of fusaric acid by fusarium species. Appl. Environ. Microbiol. 62:4039-4043
  5. Baker, A., E. Allen and H. Lutman. 2010. Corn use for ethanol increased this month. Feed Outlook, Economic Research Service, USDA. FDS-10b, February 16, 2010.
  6. Bhatnagar, D., G. A. Payne, T. E. Cleveland and J. F. Robens. 2004. Mycotoxins: current issues in USA. Page 275-280 in Meeting the Mycotoxin Menace (Ed. D. Barug, H. van Egmond, R. Lopez-Garcia, T. van Osenbruggen and A. Visconti). Wageningen Academic Publishers, The Neatherlands.
  7. Binder, E. M., L. M. Tan, L. J. Chin, J. Handl and J. Richard. 2007. Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Anim. Feed Sci. Technol. 137:265-282. https://doi.org/10.1016/j.anifeedsci.2007.06.005
  8. CAST. 2003. Mycotoxins: risks in plant, animal, and human systems. Council for Agric. Sci. Technol. Task Force Report No. 139. Ames, IA.
  9. Casteel, S. W., J. R. Turk, R. P. Cowart and G. E. Rottinghaus. 1993. Chronic toxicity of fumonisin in weanling pigs. J. Vet. Diagn. Invest. 5:413-417. https://doi.org/10.1177/104063879300500319
  10. Chaytor, A. C., M. T. See, J. A. Hansen, A. L. P. de Souza, T. F. Middleton and S. W. Kim. 2011. Effects of chronic exposure of diets with reduced concentrations of aflatoxin and deoxynivalenol on growth and immune status of pigs. J. Anim. Sci. 89:124-135. https://doi.org/10.2527/jas.2010-3005
  11. Chaytor, A. C., M. T. See, J. A. Hansen, A. L. P. de Souza, D. C. Kendall, T. F. Middleton and S. W. Kim. 2010. Efficacy of feed additives to reduce the effects of chronic exposure to aflatoxin and deoxynivalenol on growth and immune status of pigs. J. Anim. Sci. 88 (Supple. 2):818(Abstr.).
  12. Chen, Z. Y., R. L. Brown, J. S. Russin, A. R. Lax and T. E. Cleveland. 1999. A corn trypsin inhibitor with antifungal activity inhibits Aspergillus flavus $\alpha$-amylase. Phytopathology 89:902-907. https://doi.org/10.1094/PHYTO.1999.89.10.902
  13. Chen, F., Y. Ma, C. Xue, J. Ma, Q. Xie, G. Wang, Y. Bi and Y. Cao. 2008. The combination of deoxynivalenol and zearalenone at permitted feed concentrations causes serious physiological effects in young pigs. J. Vet. Sci. 9:39-44. https://doi.org/10.4142/jvs.2008.9.1.39
  14. Cheng, Y. H., C. F. Weng, B. J. Chen and M. H. Chang. 2006. Toxicity of different Fusarium mycotoxins on growth performance, immune responses and efficacy of a mycotoxin degrading enzyme in pigs. Anim. Res. 55:579-590. https://doi.org/10.1051/animres:2006032
  15. Cotty, P. J. and R. Jaime-Garcia. 2007. Influences of climate on aflatoxin producing fungi and aflatoxin contamination. Int. J. Food Microbiol. 199:109-115.
  16. Dersjant-Li, Y., M. W. A. Verstegen and W. J. J. Gerrits. 2003. The impact of low concentrations of aflatoxin, deoxynivalenol or fumonisin in diets on growing pigs and poultry. Nutr. Res. Rev. 16:223-239. https://doi.org/10.1079/NRR200368
  17. Diener, U. L., R. J. Cole, T. H. Sanders, G. A. Payne, L. S. Lee and M. A. Klich. 1987. Epidemology of aflatoxin formation by Aspergillus flavus. Annu. Rev. Phytopathol. 25:249-270. https://doi.org/10.1146/annurev.py.25.090187.001341
  18. D'Mello, J. P. F., C. M. Placinta and A. M. C. Macdonald. 1999. Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Anim. Feed Sci. Technol. 80:183-205. https://doi.org/10.1016/S0377-8401(99)00059-0
  19. Doll, S., S. Da Nicke, K. H. Ueberschar, H. Valenta, U. Schnurrbusch, M. Ganter, F. Klobasa and G. Flachowsky. 2003. Effects of graded levels of Fusarium toxin contaminated maize in diets for female weaned piglets. Arch. Anim. Nutr. 57:311-334. https://doi.org/10.1080/00039420310001607680
  20. EFSA. 2009. Review of mycotoxin-detoxifying agents used as feed additives: mode of action, efficacy and feed/food safety. Sci. Report, Parma, Italy.
  21. FAO. 2003. Worldwide regulation for mycotoxins in food and feed in 2003. Food and Agriculture Organization of the United Nations, Rome, Italy.
  22. FDA. 1994. FDA regulatory guidance for toxins and contaminants. Food and Drug Administration, Silver Spring, MD.
  23. Fink-Gremmels, J. 2006. Moulds and mycotoxins as undesirable substances in animal feeds. Page 37-50 in The Mycotoxin Factbook (Ed. D. Barug, D. Bhatnagar, H. P. van Egmond, J. W. van der Kamp, W. A. van Osenbruggen and A. Visconti). Wageningen Academic Publishers, The Netherlands.
  24. Gourama, H. and L. B. Bullerman. 1995. Aspergillus flavus and Aspergillus parasiticus: aflatoxigenic fungi of concern in foods and feeds: a review. J. Food Prot. 58:1395-1404.
  25. Goyarts, T., S. Danicke, H. J. Rothkotter, J. Spilke, U. Tiemann and M. Schollenberger. 2005. On the effects of a chronic deoxynivalenol intoxication on performance, haematological and serum parameters of pigs when diets are offered either for ad libitum consumption or fed restrictively. J. Vet. Med. 52:305-314. https://doi.org/10.1111/j.1439-0442.2005.00734.x
  26. Gutzwiller, A., J-.L. Gafner and P. Stoll. 2009. Effects of a diet containing fusarium toxins on the fertility of gilts and on bulbourethral gland weight in barrows. Arch. Anim. Nutr. 63:16-25. https://doi.org/10.1080/17450390802611537
  27. Harvey, R. B., L. F. Kubena, W. E. Huff, D. E. Corrier, D. E. Clark and T. D. Phillips. 1989. Effects of aflatoxin, deoxynivalenol, and their combinations in the diets of growing pigs. Am. J. Vet. Res. 40:602-607.
  28. Harvey, R. B., L. F. Kubena, M. H. Elissalde, D. E. Corrier, W. E. Huff, G. E. Rottinghaus and B. A. Clement. 1991. Cocontamination of swine diets by aflatoxin and diacetoxyscirpenol. J. Vet. Diagn. Invest. 3:155-160. https://doi.org/10.1177/104063879100300210
  29. Hussein, H. S. and J. M. Brasel. 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxocology 167:101-134. https://doi.org/10.1016/S0300-483X(01)00471-1
  30. Huwig, A., S. Freimund, O. Kappeli and H. Dutler. 2001. Mycotoxin detoxification of animal feed by different absorbents. Toxicol. Lett. 122:179-188. https://doi.org/10.1016/S0378-4274(01)00360-5
  31. Jiang, S., Z. Yang, W. Yang, J. Gao, F. Liu, C.C. Chen and F. Chi. 2010. Physiopathological effects of zearalenone in post-weaning female piglets with or without montmorillonite clay adsorbent. Livest. Sci. 131:130-136. https://doi.org/10.1016/j.livsci.2010.02.022
  32. Johnson, R. J. 2010. Red meat exports to strengthen in 2010. Livestock, Dairy and Poultry Outlook, Economic Research Service, USDA. LDP-M-187, January 20, 2010.
  33. Jones, F. T., M. B. Genter, W. M. Hagler, J. A. Hansen, B. A. Mowrey, M. H. Poore and L. W. Whitlow. 2007. Understanding and coping with effects of mycotoxins in livestock feed and forage. North Carolina Cooperative Extension Service, Raleigh, NC.
  34. Krogh, P. 1977. Ochratoxins. Page 489-498 in Mycotoxins in Human and Animal Health (Ed. J. V. Rodricks, C. W. Hesseltine and M. A. Mehlman). Pathotox Publishers, Inc., Park Forest South, IL.
  35. Krska, R. and E. Welzig. 2006. Mycotoxin analysis: an overview of classical, rapid and emerging techniques. Page 225-247 in The Mycotoxin Factbook (Ed. D. Barug, D. Bhatnagar, H. P. van Egmond, J. W. van der Kamp, W. A. van Osenbruggen and A. Visconti). Wageningen Academic Publishers, The Netherlands.
  36. Lemke, S. L., P. G. Grant and T. D. Phillips. 1998. Adsorption of zearalenone by organophilic montmorillonite clay. J. Agric. Food Chem. 46:3789-3796. https://doi.org/10.1021/jf9709461
  37. Lindemann, M. D., D. J. Blodgett, E. T. Kornegay and G. G. Schurig. 1993. Potential ameliorators of aflatoxicosis in weanling/growing swine. J. Anim. Sci. 71:171-178.
  38. Magan, N. and D. Aldred. 2007. Post-harvest control strategies: minimizing mycotoxins in the food chain. Int. J. Food Microbiol. 119:131-139. https://doi.org/10.1016/j.ijfoodmicro.2007.07.034
  39. Marases, W. F., W. C. A. Gelderblom, G. S. Shephard and H. F. Vismer. 2008. Mycotoxins: a global problem. Page 29-39 in Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade (Ed. J. F. Leslie, R. Bandyopadhyay, and A. Visconti). CAB International, Oxfordshire, UK.
  40. Marin, D. E., I. Taranu, R. P. Bunaciu, F. Pascale, D. S. Tudor, N. Avram, M. Sarca, I. Cureu, R. D. Criste, V. Suta and I. P. Oswald. 2002. Changes in performance, blood parameters, humoral and cellular immune responses in weanling piglets exposed to low doses of aflatoxin. J. Anim. Sci. 80:1250-1257.
  41. Meissonnier, G. M., P. Pinton, J. Laffitte, A. Cossalter, Y. Y. Gong, C. P. Wild, G. Bertin, P. Galtier and I. P. Oswald. 2008. Immunotoxicity of aflatoxin B1: Impairment of the cell-mediated response to vaccine antigen and modulation of cytokine expression. Toxicol. Appl. Pharmacol. 231:142-149. https://doi.org/10.1016/j.taap.2008.04.004
  42. Minervina, F. and M. E. Dell'Aquila. 2008. Zearalenone and reproductive function in farm animals. Int. J. Mol. Sci. 9:2570-2584. https://doi.org/10.3390/ijms9122570
  43. Murray, R. K., D. A. Bender, K. M. Botham, P. J. Kennelly, V. W. Rodwell and P. A. Weil. 2009. Harper's illustrated biochemistry. 28th ed. Mc-Graw Hill Co., New York, NY.
  44. Papaioannou, D. S., C. S. Kyriakis, A. Papasteriadis and N. Roumbies. 2002. A field study on the effect of in-feed inclusion of a natural zeolite (clinoptilolite) on health status and performance of sows/gilts and their litters. Res. Vet. Sci. 72:51-59.
  45. Pascale, M. and A. Visconti. 2008. Overview of detection methods for mycotoxins. Page 171-183 in Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade (Ed. J. F. Leslie, R. Bandyopadhyay and A. Visconti). CAB International, Oxfordshire, UK.
  46. Payne, G. A. 1998. Process of contamination by aflatoxin-producing fungi and their impact on crops. Page 279-306 in Mycotoxins in Agriculture and Food Safety (Ed. K. K. Sinha and D. Bhatnagar). Marcel Dekker, New York, NY.
  47. Pestka, J. J. 2007. Deoxynivalenol: toxicity, mechanisms and animal health risks. Anim. Feed Sci. Technol. 137:283-298. https://doi.org/10.1016/j.anifeedsci.2007.06.006
  48. Pinton, P., F. Accensi, E. Beauchamp, A. Cossalter, P. Callu, F. Grosjean and I. P. Oswald. 2008. Ingestion of deoxynivalenol (DON) contaminated feed alters the pig vaccinal immune responses. Toxicol. Lett. 177:215-222. https://doi.org/10.1016/j.toxlet.2008.01.015
  49. Pinton, P., C. Braicu, J.-P. Nougayrede, J. Laffitte, I. Taranu and I. P. Oswald. 2010. Deoxynivalenol impairs porcine intestinal barrier function and decreases the protein expression of claudin-4 through a mitogen-activated protein kinasedependent mechanism. J. Nutr. Published ahead of print September 22, 2010.
  50. Plain, R. 2008. High feed prices and record slaughter brings red ink. Page 6-12 in National Hog Farmer 2008 State of the Industry Report. Penton Media, Minneapolis, MN.
  51. Pfohl-Leszkowicz, A., T. Petkova-Bocharova, I. N. Chernozemsky and M. Castegnaro. 2002. Balkan endemic nephropathy and associated uringary tract tumours: a review on aetiological causes and the potential role of mycotoxins. Food Addit. Contam. 19:282-302. https://doi.org/10.1080/02652030110079815
  52. Ramos, A-J., J. Fink-Gremmels and E. Hernandez. 1996. Prevention of toxic effects of mycotoxins be means of nonnutritive adsorbent compounds. J. Food Prot. 59:631-641.
  53. Richard, J. L. 2007. Some major mycotoxins and their mycotoxicoses: an overview. Int. J. Food Microbiol. 119:3-10. https://doi.org/10.1016/j.ijfoodmicro.2007.07.019
  54. Rotter, B. A., B. K. Thompson, M. Lessard, H. L. Trenholm and H. Tryphona. 1994. Influence of low-level exposure to Fusarium mycotoxins on selected immunological and hematological parameters in young swine. Fundam. Appl. Toxicol. 23:117-124. https://doi.org/10.1006/faat.1994.1087
  55. Sabater-Vilar, M., H. Malekinejad, M. H. J. Selman, M. A. M. van der Doelen and J. Fink-Gremmels. 2007. In vitro assessment of absorbents aiming to prevent deoxynivalenol and zearalenone mycotoxins. Mycopathologia 163:81-90. https://doi.org/10.1007/s11046-007-0093-6
  56. Saleha, Z. A., G. A. El-Garawanyb, F. Assemb and S. El-Shibinyb. 2007. Evaluation of the efficacy of whey protein to ameliorate the toxic effects of aflatoxins in rats. Int. Dairy J. 17:854-859. https://doi.org/10.1016/j.idairyj.2006.09.014
  57. Schatzmayr, G., F. Zehner, M. Taubel, D. Schatzmayr, A. Klimitsch, A. P. Loibner and E. M. Binder. 2006. Microbiologicals for deactivating mycotoxins. Mol. Nutr. Food Res. 50:543-551. https://doi.org/10.1002/mnfr.200500181
  58. Schell, T. C., M. D. Lindemann, E. T. Kornegay, D. J. Blodgett and J. A. Doerr. 1993. Effectiveness of different types of clay for reducing the detrimental effects of aflatoxin-contaminated diets on performance and serum profiles of weanling pigs. J. Anim. Sci. 71:1226-1231.
  59. Smith, T. K. 1980. Influence of dietary fiber, protein and zeolite on zearalenone toxicosis in rats and swine. J. Anim. Sci. 50:278-285.
  60. Smith, T. K., E. G. McMillan and J. B. Castillo.1997. Effect of feeding blends of Fusarium mycotoxin-contaminated grains containing deoxynivalenol and fusaric acid on growth and feed consumption of immature swine. J. Anim. Sci. 75:2184-2191.
  61. Stillman, R., M. Haley and K. Mathews. 2009. Grain prices impact entire livestock production cycle. Amber Waves 7:24-27.
  62. Swamy, H. V. L. N., T. K. Smith, E. J. MacDonald, H. J. Boermans and E. J. Squires. 2002. Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on swine performance, brain regional neurochemistry, and serum chemistry and the efficacy of a polymeric glucomannan mycotoxin adsorbent. J. Anim. Sci. 80:3257-3267.
  63. Taranu, I., D. E. Marin, S. Bouhet, F. Pascale, J. Bailly, J. D. Miller, P. Pinton and I. P. Oswald. 2005. Mycotoxin fumonisin B1 alters the cytokine profile and decreases the vaccinal antibody titer in pigs. Toxicol. Sci. 84:301-307. https://doi.org/10.1093/toxsci/kfi086
  64. Thieu, N. Q., B. Ogle and H. Pettersson. 2008. Efficacy of bentonite clay in ameliorating aflatoxicosis in piglets fed aflatoxin contaminated diets. Trop. Anim. Health Prod. 40:649-656. https://doi.org/10.1007/s11250-008-9144-3
  65. Tiemann, U., K. P. Brussow, L. Jonas, R. Pohland, F. Schneider and S. Danicke. 2006. Effects of diets with cereal grains contaminated by graded levels of two Fusarium toxins on selected immunological and histological measurements in the spleen of gilts. J. Anim. Sci. 84:236-245.
  66. Tiemann, U. and S. Danicke. 2007. In vivo and in vitro effects of the mycotoxins zearalenone and deoxynivalenol on different non-reproductive and reproductive organs in female pigs: A review. Food Addit. Contam. 24:306-314. https://doi.org/10.1080/02652030601053626
  67. van Heugten, E., J. W. Spears, M. T. Coffey, E. B. Kegley and M. A. Qureshi. 1994. The effect of methionine and aflatoxin on immune function in weanling pigs. J. Anim. Sci. 72:658-664.
  68. Vekiru, E., C. Hametner, R. Mitterbauer, J. Rechthaler, G. Adam, G. Schatzmayr, R. Krska and R. Schuhmacherl. 2010. Cleavage of Zearalenone by Trichosporon mycotoxinivorans to a novel nonestrogenic metabolite. Appl. Environ. Microbiol. 2353-2359. https://doi.org/10.1128/AEM.01438-09
  69. Veldman, B. 2004. Mycotoxins in the animal production chain. Page 275-280 in Meeting the Mycotoxin Menace (Ed. D. Barug, H. van Egmond, R. Lopez-Garcia, T. van Osenbruggen, and A.Visconti). Wageningen Academic Publishers, The Netherlands.
  70. Whitaker, T. B., A. B. Slate and A. S. Johansson. 2005. Sampling feeds for mycotoxin analysis. Page 1-23 in The Mycotoxin Blue Book, D. Diaz, ed. Nottingham University Press, Bath, England.
  71. Wood, P. 2006. Understanding Immunology. 2nd ed. Pearson Education Limited, Harlow, England.
  72. Wu, F. 2007. Measuring the economic impacts of Fusarium toxins in animal feeds. Anim. Feed Sci. Technol. 137:363-374. https://doi.org/10.1016/j.anifeedsci.2007.06.010
  73. Wu, F. and G. P. Munkvold. 2008. Mycotoxins in ethanol coproducts: modeling economic impacts on livestock industry and management strategies. J. Agric. Food Chem. 56:3900-3911. https://doi.org/10.1021/jf072697e
  74. Yiannikouris, A., J. Francois, L. Poughon, C.-G. Dussap, G. Bertin, G. Jeminet and J.-P. Jouany. 2004. Adsorption of zearalenone by $\beta$-D-glucans in the Saccharomyces cerevisiae cell wall. J. Food Prot. 67:1195-1200.

Cited by

  1. Mould and mycotoxin contamination of pig feed in northwest Croatia vol.28, pp.3, 2012, https://doi.org/10.1007/s12550-012-0130-2
  2. The Use of Feed Additives to Reduce the Effects of Aflatoxin and Deoxynivalenol on Pig Growth, Organ Health and Immune Status during Chronic Exposure vol.5, pp.7, 2013, https://doi.org/10.3390/toxins5071261
  3. Zearalenone Altered the Serum Hormones, Morphologic and Apoptotic Measurements of Genital Organs in Post-weaning Gilts vol.28, pp.2, 2015, https://doi.org/10.5713/ajas.14.0329
  4. Mineral adsorbents for prevention of mycotoxins in animal feeds vol.33, pp.3, 2014, https://doi.org/10.3109/15569543.2014.905604
  5. Deoxynivalenol: signaling pathways and human exposure risk assessment—an update vol.88, pp.11, 2014, https://doi.org/10.1007/s00204-014-1354-z
  6. Effects of Modified Maifanite on Zearalenone Toxicity in Female Weaner Pigs vol.14, pp.2, 2015, https://doi.org/10.4081/ijas.2015.3597
  7. Effects of Feeding Barley Naturally Contaminated with Fusarium Mycotoxins on Growth Performance, Nutrient Digestibility, and Blood Chemistry of Gilts and Growth Recoveries by Feeding a Non-contaminated Diet vol.28, pp.5, 2015, https://doi.org/10.5713/ajas.14.0707
  8. An impact of Deoxynivalenol produced by Fusarium graminearum on broiler chickens vol.102, pp.4, 2018, https://doi.org/10.1111/jpn.12883
  9. Dietary arginine supplementation enhances intestinal expression of SLC7A7 and SLC7A1 and ameliorates growth depression in mycotoxin-challenged pigs vol.46, pp.4, 2014, https://doi.org/10.1007/s00726-013-1643-5
  10. DEOXYNIVALENOL, A TRICHOTHECENE MYCOTOXIN: REVIEW OF ITS MASKED FORM, CONTAMINATION IN CEREAL-BASED FEED, AND MASS SPECTROMETRY ANALYTICAL METHODS vol.84, pp.3, 2011, https://doi.org/10.31482/mmsl.2015.012
  11. Evaluation of a mycotoxin adsorbent in swine diets containing barley naturally contaminated with Fusarium mycotoxins vol.29, pp.3, 2011, https://doi.org/10.17533/udea.rccp.v29n3a02
  12. Mycotoxins - climate impact and steps to prevention based on prediction vol.68, pp.1, 2011, https://doi.org/10.2478/acve-2018-0001
  13. Mycotoxin contamination of diets for beef cattle finishing in feedlot vol.48, pp.None, 2011, https://doi.org/10.1590/rbz4820190079
  14. Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation vol.11, pp.6, 2011, https://doi.org/10.3390/toxins11060330
  15. Multiannual occurrence of mycotoxins in feed ingredients and complete feeds for pigs in the Czech Republic vol.88, pp.3, 2019, https://doi.org/10.2754/avb201988030291
  16. Physico-Chemical Properties of Clay Minerals and Their Use as a Health Promoting Feed Additive vol.9, pp.10, 2019, https://doi.org/10.3390/ani9100714
  17. Effect of acid-treated and hexadecyltrimethylammonium bromide-modified montmorillonites on adsorption performance of mycotoxins vol.27, pp.4, 2011, https://doi.org/10.1007/s11356-019-07118-2
  18. Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol vol.12, pp.5, 2011, https://doi.org/10.3390/toxins12050311
  19. Effects of Chronic Exposure to Low Levels of Dietary Aflatoxin B1 on Growth Performance, Apparent Total Tract Digestibility and Intestinal Health in Pigs vol.11, pp.2, 2021, https://doi.org/10.3390/ani11020336
  20. Mycotoxin Occurrence, Toxicity, and Detoxifying Agents in Pig Production with an Emphasis on Deoxynivalenol vol.13, pp.2, 2011, https://doi.org/10.3390/toxins13020171
  21. Understanding intestinal health in nursery pigs and the relevant nutritional strategies vol.34, pp.3, 2021, https://doi.org/10.5713/ab.21.0010
  22. Investigation of the efficacy of mycotoxin-detoxifying additive on health and growth of newly-weaned pigs under deoxynivalenol challenges vol.34, pp.3, 2011, https://doi.org/10.5713/ajas.20.0567
  23. Equations to Predict Growth Performance Changes by Dietary Deoxynivalenol in Pigs vol.13, pp.5, 2021, https://doi.org/10.3390/toxins13050360
  24. Physiological Effects of Deoxynivalenol from Naturally Contaminated Corn on Cerebral Tryptophan Metabolism, Behavioral Response, Gastrointestinal Immune Status and Health in Pigs Following a Pair-Feed vol.13, pp.6, 2011, https://doi.org/10.3390/toxins13060393
  25. Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs vol.13, pp.7, 2011, https://doi.org/10.3390/toxins13070442