Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant and Anti-inflammatory Activities of Ethanol Extracts from Medicinal Herb Mixtures

단일 생약재 및 생약복합제 에탄올추출물의 항산화 및 항염증 활성

  • Lee, Sung-Gyu (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Jeong, Hyun-Jin (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Lee, Eun-Ju (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Kim, Jong-Boo (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu)
  • 이성규 ((주)엔유씨전자 바이오연구소) ;
  • 정현진 ((주)엔유씨전자 바이오연구소) ;
  • 이은주 ((주)엔유씨전자 바이오연구소) ;
  • 김종부 ((주)엔유씨전자 바이오연구소) ;
  • 최상원 (대구가톨릭대학교 식품영양학과)
  • Received : 2010.10.19
  • Accepted : 2010.12.28
  • Published : 2011.04.30
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Abstract

In this study, antioxidant and anti-inflammatory effects of ethanol extracts from medicinal herb mixtures were investigated in vitro. Contents of total polyphenols in ethanol extracts from Houttuynia cordata Thunb. (HC), Carthamus tinctorius L. (CT), and MIX-2 (Houttuynia cordata Thunb., Acanthopanax gracilistylus Smith, Carthamus tinctorius L., Kalopanax pictus) were 58.98, 60.79 and 57.74 mg/g respectively. Radical scavenging activities of the ethanol extracts were examined using ${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl radicals and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay. Anti-inflammatory effects of ethanol extracts from medicinal herbs composites were examined using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and nitric oxide (NO) inhibition assays. The ethanol extracts from medicinal herb mixtures showed higher 5-LO and COX-2 inhibition activities than those from single medicinal herbs. The ethanol extracts from medicinal herb mixtures also showed strong anti-inflammatory activity through inhibition of NO production.

본 연구에서는 문헌을 통하여 항산화 혹은 항염증 활성을 가지는 6종의 생약재와 이들 생약재를 혼합하여 제조한 생약복합제의 항산화 및 항염증 활성을 측정하였다. 총 폴리페놀 함량은 어성초, 홍화, MIX-2 에탄올추출물이 각각 58.98, 60.79, 57.74 mg/g의 함량으로 다른 시료들 보다 높게 측정되었다. 또한 플라보노이드 함량도 어성초(HC), 홍화(CT), MIX-2에탄올추출물이 각각 36.86, 19.42, 14.74 mg/g으로 높게 측정되었다. 단일 생약재와 생약복합제 에탄올추출물의 라디칼 소거능을 측정한 결과 DPPH 라디칼의 RC50 값은 어성초, 홍화, MIX-2 에탄올추출물에서 각각 69.64, 50.70, $63.49{\mu}g/mL$로 추출물 가운데 우수한 DPPH 라디칼 소거능을 보였고, ABTS 라디칼의 $RC_{50}$ 값도 어성초, 홍화, MIX-2가 각각 24.06, 18.91, $41.61{\mu}g/mL$로 DPPH 라디칼 소거능과 유사한 경향을 보였다. 시료들의 항염증 효능을 측정하기 위해 5-LO 및 COX-2 효소 저해활성을 측정한 결과 단일 생약재 에탄올추출물보다 생약복합제 에탄올추출물이 5-LO와 COX-2 효소를 동시에 저해하는 효과가 증가되는 것으로 나타났다. 염증유발 효소 저해활성이 우수했던 생약복합제 에탄올추출물들의 NO 소거활성을 비교한 결과, MIX-2 에탄올추출물이 $50{\mu}g/mL$의 농도에서부터 유의적으로 NO의 생성을 저해하는 것을 확인하였다. 따라서 단일 생약재 보다는 생약복합제가 서로 혼합되면서 시너지 효과가 나타나는 것으로 생각되며 앞으로 명확한 기전에 대한 연구들이 지속된다면 기능성 식품 소재로서의 이용이 가능할 것으로 생각된다.

Keywords

References

  1. Sadaki O. The development of functional and materials. Bioindustry 13: 44-50 (1996)
  2. Cadenas E, Davies KJA. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Bio. Med. 29: 222-230 (2000) https://doi.org/10.1016/S0891-5849(00)00317-8
  3. Moini G, Parker L, Saris NL. Antioxidant and prooxidant activities of $\alpha$-lipoic acid and dihydrolipoic acid. Toxicol. Appl. Pharm. 182: 84-90 (2002) https://doi.org/10.1006/taap.2002.9437
  4. Giles GI, Tasker KM, Jacob C. Hypothesis: The role of reactive sulfur species in oxidative stress. Free Radical Bio. Med. 31:1279-1283 (2001) https://doi.org/10.1016/S0891-5849(01)00710-9
  5. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutr. Res. 18: 872-879 (2002)
  6. Fubini B, Hubbard A. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis. Free Radical Bio. Med. 34: 1507-1516 (2003) https://doi.org/10.1016/S0891-5849(03)00149-7
  7. Barene AL. Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytolune. J. Am. Oil Chem. Soc. 52:59-63 (1975) https://doi.org/10.1007/BF02901825
  8. Choe SY, Yang KH. Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxy anisol (BHA). Korean J. Food Sci. Technol. 14: 283-288 (1982)
  9. Pratt DE, Huang MT, Ho ST, Lee CY. Phenolic Compounds in Food and Their Effects on Health (II). antioxidants and cancer prevention, Washington DC, USA. pp. 54-71 (1992)
  10. Kim EC, Ahn SY, Hong ES, Li GH, Kim EK, Row KH. Extraction of whitening agents from natural plants and whitening effect. J. Korean Ind. Eng. Chem. 16: 348-353 (2005)
  11. Funk CD. Prostaglandins and leukotrienes; advances in eicosanoid biology. Science 294: 1871-1875 (2001) https://doi.org/10.1126/science.294.5548.1871
  12. Kaliora AC, Dedoussis GVZ, Schimidit H. Dietary antioxidants in preventing atherogenesis. Atherosclerosis 187: 1-17 (2006) https://doi.org/10.1016/j.atherosclerosis.2005.11.001
  13. Yang MS, Ha YL, Nam SH, Choi SU, Jang DS. Screening of domestic plants with antibacterial activity. J. Korean Soc. Agric. Chem. Biotechnol. 38: 584-589 (1995)
  14. Kim KD. Research of oriental medicine plant on antioxidation and ultraviolet rays absorption. J. Korean Soc. Cosm. 10: 145-153 (2004)
  15. Li Y, Metori K, Koike K, Che QM, Takahashi S. Improvement in the turnover rate of the stratum comeum in false aged model rats by the administration of geniposide acid in Eucommia ulmoides oliver leaf. Biol. Pharm. Bull. 22: 582-585 (1999) https://doi.org/10.1248/bpb.22.582
  16. Chen YY, Liu JF, Chen CM, Chao PY, Chang TJ. A study of antioxidative and antimutagenic effects of Houttuynia cordata thunb using an oxidized frying oil-fed model. J. Nutr. Sci. Vitaminol. 49: 327-333 (2003) https://doi.org/10.3177/jnsv.49.327
  17. Song JH, Kim MJ, Kwon HD, Park IH. Antimicrobial activity of fractional ectracts from Houttuynia cordata root. J. Korean Soc. Food Sci. Nutr. 32: 1053-1058 (2003) https://doi.org/10.3746/jkfn.2003.32.7.1053
  18. Cho EJ, Yokozawa T, Rhyu DY, Kim HY, Shibahara N, Park JC. The inhibitory effects of 12 medicinal plants and their component on lipid perocidation. Am. J. Chinese Med. 31: 907-917 (2003) https://doi.org/10.1142/S0192415X03001648
  19. Lee KH, Nam JO, Yoon WH. Effect of protein-bond polysacharide isolated from Acanthopanax senthopanax in reducing the toxic effect of cisplatin. Korean J. Pharmacogn. 38: 1-17 (2007)
  20. Singh RK, Pandey BL. Further study of anti-inflammatory effects of Abies pindrow. Phytother. Res. 11: 535-537 (1997) https://doi.org/10.1002/(SICI)1099-1573(199711)11:7<535::AID-PTR146>3.0.CO;2-F
  21. Kawashima S, Hayashi M, Takii T, Kimura H, Zhang HL, Nagatsu A, Sakakibara J, Murata K, Oomoto Y, Onozaki K. Serotonin derivative, N-($\rho$-coumaroyl) serotonin, inhibits the production of TNF-$\alpha$, IL-1$\alpha$. IL-1$\beta$, and IL-6 by endotoxin-stiumlated human blood monocytes. J. Interf. Cytok. Res. 18: 423-428 (1998) https://doi.org/10.1089/jir.1998.18.423
  22. Zhang HL, Nagatsu A, Watanabe T, Sakakibara J, Okutama H. Antioxidative dompounds isolated from safflower (Carthamus tinctorious L.) oil cake. Chem. Pharm. Bull. 45: 1910-1914 (1997) https://doi.org/10.1248/cpb.45.1910
  23. Bae SJ, Shim SM, Park YJ, Lee JY, Chang EJ, Choi SW. Cytotoxicity of phenolic compounds isolated from seeds of safflower (Carthamus tinctorius L.) on cancer cell lines. Food Sci. Biotechnol. 11: 140-146 (2002)
  24. Choi JW, Huh K, Kim SH, Lee KT, Lee HK, Park HJ. Kalopanaxsaponin A from kalopanax pictus, a potent antioxidant in the rheumatoidal rat treated with Freund's complete adjuvant reagent. J. Ethnopharmacol. 79: 113-118 (2002) https://doi.org/10.1016/S0378-8741(01)00382-8
  25. Choi L, Han YN, Lee KT, Park KY, Kwak TS, Kwon SH, Park HL. Anti-lipid peroxidative principles from the stem bark of Kalopanax pictus Nakai. Arch. Pharm. Res. 24: 536-540 (2001) https://doi.org/10.1007/BF02975161
  26. Lee EB, Li DW, Hyun JE, Kim IH, Whang WK. Anti-inflammatory activity of methanol extract of Kalopanax pictus bark and its fractions. J. Ethnopharmacol. 77: 197-201 (2001) https://doi.org/10.1016/S0378-8741(01)00301-4
  27. Lee MW, Kim SU, Hahn DR. Antifungal acticity of modified hederagenin glycosides from the leaves of Kalopanax pictum var. chinense. Biol. Pharm. Bull. 24: 718-719 (2001) https://doi.org/10.1248/bpb.24.718
  28. Kim DH, YU KW, Bae EA, Park HJ, Choi JW. Metabolism of kalopanax B and H by human intestinal bacteria and antidiabetic activity of their metabolites. Biol. Pharm. Bull. 21: 360-365 (1998) https://doi.org/10.1248/bpb.21.360
  29. Bansky D, Barolet R. Chinese Herbal Medicine Formulas and Strategies. Eastland Press, Seattle, WA, USA. pp. 7-8 (1990)
  30. Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-249 (1912)
  31. Nieva, Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114 (2000) https://doi.org/10.1016/S0378-8741(99)00189-0
  32. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  33. Charmichael J, Degraff WG, Gazdar AF, Minna JD, Michell JB. Evaluation of a tetrazolium based semiautomated colorimetric assay, assessment of chemosensitivity testing. Cancer Res. 47: 936-941 (1987)
  34. Green LC, Wagner DA, Glogowski J. Analysis of nitrate, and nitrate in biological fluids. Anal. Biochem. 126: 131-138 (1982) https://doi.org/10.1016/0003-2697(82)90118-X
  35. Lee MH, Jo DJ, Yoon SR, Lee GD. Physicochemical properties of functional herb mixtures. J. Korean Soc. Food Sci. Nutr. 36: 1571-1577 (2007) https://doi.org/10.3746/jkfn.2007.36.12.1571
  36. Cho SY, Han YB, Shin KH. Screening for antioxidant activity of edible plants. J. Korean Soc. Food Sci. Nutr. 30: 133-137 (2001)
  37. Yu MH, Im HG, Lee HJ, Ji YJ, Lee IS. Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis Rehder. Korean J. Food Sci. Technol. 38: 128-134 (2006)
  38. Ahn SI, Heuing BJ, Son JY. Antioxidative activity and nitritescavenging abilities of some phenolic compounds. Korean J. Food Cookery Sci. 23: 19-24 (2007)
  39. Lee SG, Yu MH, Lee SP, Lee IS. Antioxidant activities and induction of apoptosis by methanol extracts from avocado. J. Korean Soc. Food Sci. Nutr. 37: 269-275 (2008) https://doi.org/10.3746/jkfn.2008.37.3.269
  40. Kang YH, Park YK, Oh SR, Moon KD. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27: 978-984 (1995)
  41. Kim KM. Screening of anti-allergic agents using lipoxygenase assay and degranulation marker. J. Drug Res. 5: 45-48 (1996)
  42. Lee YS, Jang SI. Study on the anti-inflammatory effects of the remedy prescripted with Lonicerae Flos and Scutellariae Radix in U937 cells. Korean J. Oriental Med. Prescription 18: 121-132 (2010)
  43. Gross SS, Wolin MS. Nitric oxide: Pathophysiological mechanisms. Annu. Rev. Physiol. 57: 737-769 (1995) https://doi.org/10.1146/annurev.ph.57.030195.003513
  44. Stuehr DJ. Mammalian nitric oxide synthases. Biochem. Biophys. Acta 1411: 271-230 (1999)
  45. Halliwell B. Reactive oxygen species and the central nervous system. J. Neurochem. 59: 1609-1623 (1992) https://doi.org/10.1111/j.1471-4159.1992.tb10990.x

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