Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidative Activity and Chemical Characteristics of Leaf and Fruit Extracts from Thuja orientalis

측백나무 잎.열매 추출물의 이화학적 특성 및 항산화 효과

  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Heo, Su-Jin (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Kang, Min-Jung (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Lee, Jae-Hong (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 허수진 (동아대학교 대학원 의생명과학과) ;
  • 강민정 (동아대학교 대학원 의생명과학과) ;
  • 이재홍 (동아대학교 대학원 의생명과학과) ;
  • 차재영 (대선주조(주) 기술연구소) ;
  • 조영수 (동아대학교 생명공학과)
  • Received : 2011.03.09
  • Accepted : 2011.04.14
  • Published : 2011.05.30
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Abstract

The contents of bioactive materials (e.g. polyphenolics compounds, flavonoids, minerals, and fatty acids) and antioxidative activities (DPPH (${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl) free radical scavenging activity, peroxidation of linoleic acid and rat hepatocyte microsome, and Fe/Cu reducing power) were tested by in vitro experimental models using water, ethanol and methanol extracts of leaves (TOL) and fruits (TOF) from Thuja orientalis. Methanol extract from TOL showed the highest extraction yield (12.90%) as well as contents of polyphenolic compounds (16.02%) and flavonoids (0.25%). Major minerals were Ca, K, and Mg. Major fatty acids were palmitic and lauric acids in TOL and palmitic and decanoic acids in TOF. In oxidation of in vitro models using DPPH free radical scavenging activity, Fe/Cu reducing power, $Fe^{2+}$/ascorbate-induced linolenic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods, and autooxidation of rat hepatic microsomes membrane, anti-oxidative activities were stronger in all extracts of TOL than in those of TOF in a dose-dependent manner. From these results, methanol extract of TOL was shown to have the most potent anti-oxidative properties and the highes content of antioxidative compounds such as polyphenolic compounds and flavonoids.

측백나무 잎 및 열매 물, 에탄올 및 메탄올 추출물의 생리활성 물질(총 폴리페놀 화합물, 플라보노이드, 미네랄, 지방산 조성) 분석과 항산화 활성(DPPH free radical scavenging 활성, Cu/Fe-환원력, 간 조직 microsome 생체막 및 linoleic acid 과산화지질)을 측정하였다. 측백나무 잎의 메탄올 추출물에서 추출 수율(12.90%), 폴리페놀 화합물(16.02%) 및 플라보노이드(0.25%) 함량이 가장 높았다. 측백나무 잎 및 열매의 주요 미네랄은 Ca, K, 및 Mg이었다. 측백나무 잎의 주요 지방산은 palmitic acid 및 lauric acid였으며, 열매는 palmitic acid 및 decanoic acid가 높은 함량을 보였다. DPPH free radical scavenging 활성, Cu/Fe-환원력, 간 조직 microsome 생체막 및 linoleic acid의 과산화 지질 측정에 의한 항산화 활성은 측백나무 열매보다는 잎 추출물에서 높았으며, 시료 처리 농도 의존적으로 활성이 증가되는 것으로 나타났다. 이상의 실험 결과에서 측백나무 잎의 메탄올 추출물에서 높은 항산화 활성이 있었으며, 이는 폴리페놀 화합물과 플라보노이드와 같은 항산화 활성을 나타내는 생리활성 성분을 많이 함유하고 있는 것으로 나타나 향후 건강기능식품이나 화장품의 천연 항산화제 소재개발에 유용하게 사용될 것으로 사료된다.

Keywords

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