Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibition of Adipocyte Differentiation and Adipogenesis by Aged Black Garlic Extracts in 3T3-L1 Preadipocytes

흑마늘 추출물에 의한 3T3-L1 지방전구세포의 분화 및 adipogenesis 억제에 관한 연구

  • Park, Jung-Ae (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Park, Cheol (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Han, Min-Ho (Department of Biomaterial Control, Graduate School, Dong-Eui University) ;
  • Kim, Byung-Woo (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Chung, Yoon-Ho (Duksan B&F Co. LTD.) ;
  • Choi, Yung-Hyun (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
  • 박정애 (동의대학교 블루바이오 소재 개발 센터) ;
  • 박철 (동의대학교 블루바이오 소재 개발 센터) ;
  • 한민호 (동의대학교 대학원 바이오물질제어학과) ;
  • 김병우 (동의대학교 블루바이오 소재 개발 센터) ;
  • 정윤호 (덕산비엔에프주식회사) ;
  • 최영현 (동의대학교 블루바이오 소재 개발 센터)
  • Received : 2011.02.07
  • Accepted : 2011.02.12
  • Published : 2011.05.30
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Abstract

Garlic (Allium sativum) has been used as a source food as well as a traditional folk medicine ingredient since ancient times. Aged black garlic is a type of fermented garlic and is expected to have stronger anticancer and antioxidant activities than raw garlic. However, the mechanisms of their inhibitory effects on adipocyte differentiation and adipogenesis are poorly understood. In the present study, the effects and mechanisms of water extracts of raw garlic (WERG) and aged black garlic (WEABG) on adipocyte differentiation and adipogenesis in 3T3-L1 preadipocytes were investigated. Treatment with WEABG significantly suppressed terminal differentiation of 3T3-L1 preadipocytes in a dose-dependent manner as confirmed by a decrease in lipid droplet number and lipid content through Oil Red O staining, however WERG had no such effect. In addition, WEABG reduced accumulation of cellular triglyceride, which is associated with a significant inhibition of key pro-adipogenic transcription factors including peroxisome proliferator-activated receptor ${\gamma}$ (PPAR${\gamma}$), cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins ${\alpha}$ (C/EBP${\alpha}$) and C/EBP${\beta}$. Taken together, these results provide important new insight that aged black garlic might inhibit adipogenesis by suppressing the pro-adipogenic transcription factors in 3T3-L1 preadipocytes, and further studies will be needed to identify the active compounds that confer the anti-obesity activity of aged black garlic.

본 연구에서는 3T3-L1 지방전구세포에서 생마늘 및 흑마늘 열수추출물이 insulin, dexamethasone 및 IBMX 등과 같은 분화유도인자에 의하여 유발되는 lipid droplet 및 triglyceride 생성 등과 같은 지방세포로의 분화와 함께 이러한 분화과정에 중요한 역할을 하는 adipogenic transcription factor인 PPAR${\gamma}$, C/EBP${\alpha}$ 및 C/EBP${\beta}$의 발현에 어떠한 영향을 미치는지를 조사하였다. 이를 위하여 성숙한 지방세포에서 나타나는 lipid droplet의 생성에 어떠한 영향을 미치는 지를 확인한 결과, WEABG 처리군에서 lipid droplet의 생성이 현저하게 억제되는 것으로 나타났다. Lipid droplet과 함께 성숙한 지방세포에서 나타나는 특징 중 하나로서 비만의 원인으로 작용하는 지방세포 내의 저장 지방인 triglyceride 생성 정도를 확인한 결과에서도 WEABG 처리군에서 triglyceride 억제효과가 강하게 나타났다. 또한 WERG 및 WEABG-A는 분화유도인자에 의하여 유발된 PPAR${\gamma}$, C/EBP${\alpha}$ 및 C/EBP${\beta}$의 발현 억제에는 큰 영향을 미치지 못하였지만 WEABG는 그들의 발현을 전사 및 번역 수준에서 현저하게 억제시켰다. 이는 WEABG가 adipogenic transcription factor의 발현을 효과적으로 억제함으로서 lipid droplet 및 triglyceride 생성을 억제하여 지방세포로의 분화를 막는 항비만 효능을 가질 수 있음을 의미하는 결과이다. 본 연구 결과는 흑마늘의 비만억제 가능성을 제시하는 것으로서 항비만 기전에 대한 생화학적 해석 및 이를 활용한 향후 지속적인 연구를 위한 귀중한 자료로서 그 가치가 매우 높을 것으로 생각된다.

Keywords

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