Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Sex Identification of the First Incubated Chicks of the Crested Ibis Nipponia nippon in Korea

한국 최초 인공번식에 성공한 따오기의 성별구별

  • Kim, Kyung-A (College of Natural Sciences, Kyungpook National University) ;
  • Cha, Jae-Seok (College of Natural Sciences, Kyungpook National University) ;
  • Kim, Tae-Jwa (College of Natural Sciences, Kyungpook National University) ;
  • Kim, Kyung-Min (School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University) ;
  • Park, Hee-Cheon (College of Natural Sciences, Kyungpook National University)
  • 김경아 (경북대학교 자연과학대학 생물학과) ;
  • 차재석 (경북대학교 자연과학대학 생물학과) ;
  • 김태좌 (경북대학교 자연과학대학 생물학과) ;
  • 김경민 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 박희천 (경북대학교 자연과학대학 생물학과)
  • Received : 2011.02.20
  • Accepted : 2011.05.12
  • Published : 2011.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

In October 2008, a pair of Crested ibis Nipponia nippon, an endangered avian species in the world, was donated to Korea from China. They have since been the subject of a successful program to incubate chicks for the first time in South Korea. This study was carried out to determine the sex of chicks from the Crested ibis through polymerase chain reaction (PCR) using the sex-related gene and the chromodomain helicase DNA binding protein (CHD) gene. The result of the CHD gene, which was used with a single set of primers and a restriction enzyme treatment after the PCR process, was more accurate in identifying the gender of the Crested ibis. In addition, we compared the CHD gene sequences with the previously reported sequences and found 1~2 different bases between females (CI2, CI4, CI5, and CI6) than in studies previously reporting female sequences.

세계적 멸종위기종인 따오기(Nipponia nippon)는 2008년 10월에 중국에서 1쌍이 도입된 후 한국최초로 인공번식에 성공하였다. 본 연구는 따오기의 sex-related gene과 Chromodomain Helicase DNA Binding Protein gene (CHD gene)을 가지고 polymerase chain reaction (PCR)을 수행하여 새로 태어난 따오기 유조의 성별을 확인하고자 하였다. 본 연구에서는 따오기의 성별 확인을 위해 PCR후 제한효소의 처리 방법과 P2과 P8를 이용한 PCR 방법을 실시하였을 때 더 정확한 결과가 나타남을 알 수 있었다. 그리고 CHD gene의 염기서열을 선행연구와 비교해 본 결과, 암컷의 염기서열에서 1~2 base pairs 차이가 나타남을 알 수 있었다.

Keywords

References

  1. Archibald, G. W. and S. D. H. Lantis. 1981. Japanese Crested Ibis -Nipponia nippon. pp. 37-46, The present status / Rare birds of the Far East. Vladivostok.
  2. Dubiec, A. and M. Zagalska. 2006. Molecular techniques for sex identification in birds. Biological Lett. 43, 3-12.
  3. Griffiths, R., S. Daan, and C. Dijkstra. 1996. Sex identification in birds using two CHD genes. Proc. Biol. Sci. 263, 1251-1256. https://doi.org/10.1098/rspb.1996.0184
  4. Griffiths, R., C. D. Mike, O. Kate, and J. G. D. Robert. 1998. A DNA test to sex most birds. Mol. Ecol. 7, 1071-1075. https://doi.org/10.1046/j.1365-294x.1998.00389.x
  5. He, L. P., Q. H. Wan, S. G. Fang, and Y. M. Xi. 2006.Development of novel microsatellite loci and assessment of genetic diversity in the endangered Crested Ibis, Nipponia Nippon. Conserv. Genet. 7, 157-160. https://doi.org/10.1007/s10592-005-6790-0
  6. Itoh, Y., A. Ogawa, K. Murata, T. Hosoda, and S. Mizuno. 1997. Identification of the sex of Oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequence. Genes Genet. Syst. 72, 51-56. https://doi.org/10.1266/ggs.72.51
  7. Kikuchi, M. and S. Ishii. 2002. Sex idenntification of the Japanese Crested Ibis, Nipponia nippon, by Chromodomain Helicase DNA binding protein (CHD) Gene Analysis. J. Yamashina Inst. Ornithol. 33, 189-197. https://doi.org/10.3312/jyio1952.33.189
  8. King, C. E. 1992. Breeding the Oriental white stork, Ciconia boyciana, at Vogelpark Walsrode, German. Zool. Gart. (N.F.) 62, 339-350.
  9. Li, M., C. Ding, F. Wei, S. Meng, Y. Xi, and B. Lu. 2001. Sex-related gene and sex identification of Crested Ibis Nipponia nippon (Ciconiiformes: Threskiornithidae). Chin. Sci. Bull. 46, 669-671. https://doi.org/10.1007/BF03182832
  10. Li, M., C. Ding, S. Meng, and H. B. Tamate. 2002. NCBI LOCUS No. AB045309.
  11. Li, X., H. Tian, and D. Li. 2009. Why the crested ibis declined in the middle twentieth century. Biodivers. Conserv. 18, 2165-2172. https://doi.org/10.1007/s10531-009-9580-z
  12. Murata, K., T. Hasegawa, and K. Matsushima. 1993. The cause of death of Eastern white stork (Ciconia ciconia boyciana ) in captivity. J. Jpn. Assoc. Zool. Aqua. 34, 23-27.
  13. Vie, J., C. Hilton-Taylor, and S. Stuart. 2009. Wildlife in a Changing Woild - An Analysis of he 2008 IUCN Red Lis of Threstened Species, Gland, Switzerland, IUCN. pp. 180.
  14. Yamashina, Y. 1975. The feeding of Japanese Crested Ibis. pp.161-164, In Templ, S. A. (ed.), Endangered Birds. Madison : University of Wisconsin Press.
  15. Yamashima, Y. and G. Nakanishi. 1983. Nipponia nippon. pp. 301, Newton Book. Tokyo. Kyoikusha (In Japanese).