한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제56권1호
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  • Pages.35-43
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  • 2011
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
권호 표지
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Rapd Analysis of Trichoderma Isolates for Superior Selection for Biopesticide Preparation

  • 투고 : 2010.11.01
  • 발행 : 2011.03.30
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초록

Thirty five isolates of Trichoderma species collected from seven different locations of Bangladesh were studied for morphological characters and molecular variation. Mycelial diameters of the isolates varied from 8.28 cm to 9.00 cm. Based on colony colour, isolates were grouped into five such as dark green, green, light green, yellowish green and whitish green. Maximum isolates were green and light green. On the basis of growth habit and colony consistency, the isolates were categorized into three groups, in which most species had fast growth and were compact in appearance. PCR-based Random Amplified Polymorphic DNA (RAPD) technique employing 3 decamer primers produced 36 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high level of genetic diversity among the isolates. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei's (1972) genetic distance produced 2 main clusters (13 isolates in cluster 1 and 22 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of biopesticide and decomposition of municipality waste.

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피인용 문헌

  1. NativeTrichodermastrains isolated from Bangladesh with broad spectrum antifungal action against fungal phytopathogens vol.49, pp.1-4, 2016, https://doi.org/10.1080/03235408.2016.1147121