KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지
DOI QR코드

DOI QR Code

Rapd Analysis of Trichoderma Isolates for Superior Selection for Biopesticide Preparation

  • Received : 2010.11.01
  • Published : 2011.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Thirty five isolates of Trichoderma species collected from seven different locations of Bangladesh were studied for morphological characters and molecular variation. Mycelial diameters of the isolates varied from 8.28 cm to 9.00 cm. Based on colony colour, isolates were grouped into five such as dark green, green, light green, yellowish green and whitish green. Maximum isolates were green and light green. On the basis of growth habit and colony consistency, the isolates were categorized into three groups, in which most species had fast growth and were compact in appearance. PCR-based Random Amplified Polymorphic DNA (RAPD) technique employing 3 decamer primers produced 36 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high level of genetic diversity among the isolates. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei's (1972) genetic distance produced 2 main clusters (13 isolates in cluster 1 and 22 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of biopesticide and decomposition of municipality waste.

Keywords

References

  1. Ashrafuzzaman, H.A. 1976. Laboratory manual of plant pathology. First edition. Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh. pp 94.
  2. Askew, D.J. and M.D. Laing. 1993. An adapted selective medium for the quantitative isolation of Trichoderma species. Plant Path. 42: 686-690. https://doi.org/10.1111/j.1365-3059.1993.tb01553.x
  3. Costa, Ana Bolena Lima da and Larissa Brandao Goes. 2002. Randomly amplified polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani. Braz. Arch. Biol. Technol. 45(2): 536-542.
  4. Fokkema, N.J. 1995. Strategies for biocontrol of foliar fungal diseases. In M. Manka (ed.), Environmental Biotic Factors in Integrated Plant Disease Control. The Polish Phytopathological Society, Poznan. pp. 69-79.
  5. Harman, G.E. 2000. The myths and dogmas of biocontrol: changes in perceptions derived from research on Trichoderma harzianum strain T-22. Plant Disease. 84: 377-393. https://doi.org/10.1094/PDIS.2000.84.4.377
  6. Kubicek, C.P. and G.E. Harman. 1998. Trichoderma and Gliocladium. Taylor & Francis Ltd, 1 Gunpowder Square, London, EC4A 3DE. pp. 3-30.
  7. Mailer, R., R. Scarth, and B. Fristensky. 1994. Discrimination among cultivars of rapeseed (Brassica napus L) using DNA polymorphisms amplified from arbitrary primers. Theor. Appl. Genet. 87: 697-704.
  8. Meah, M.B. 2007. Formulation of Bio-pesticides in controlling Phomopsis fruit rot, foot/collar rot and shoot and fruit borer of eggplant. Annual Research Report (2006-2007), USDABangladesh collaborative research. pp 4-11.
  9. Meah, M.B. and M.M. Islam. 2005. Development of an integrated approach for management of Phomopsis blight and fruit rot of eggplant in Bangladesh. Annual Research Report (2004-2005). Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. pp. 42.
  10. Nei, M. 1972. Genetic distance between populations. American Naturalist. 106: 283-292. https://doi.org/10.1086/282771
  11. Radwan, M.B. and I. Mohammad. 2006. Biological control of Sclerotium rolfsii by using indigenous Trichoderma spp. isolates from Palestine. Hebron Univ. Res. J. 2: 27-47.
  12. Raeder, U. and P. Broda. 1985. Rapid preparation of DNA from filamentous fungi. Lett. in Appl. Microbiol. 1: 17-20. https://doi.org/10.1111/j.1472-765X.1985.tb01479.x
  13. Rifai, M.A. 1969. A revision of the genus Trichoderma. Mycol. Papers. 116: 1-56.
  14. Samuels, G. J. and D.J. Lodge. 1996. Three species of Hypocrea with stipitate stromata and Trichoderma anamorphs. Mycologia. 88: 302-315. https://doi.org/10.2307/3760935
  15. Schlick, A., K. Kuhls and K. Messner. 1994. Fingerprinting reveals gamma-ray induced mutations in fungal DNA: implications for identification of patent strains of Trichoderma harzianum. Curr. Genet. 26: 74-78. https://doi.org/10.1007/BF00326307
  16. Singh, S.K. and V.P. Sharma. 2006. Molecular characterization of Trichoderma taxa causing green mould disease in edible mushrooms. Curr. Sci. 90(3): 427-431.
  17. Sultana, N., M.S.M. Chowdhury, and I. Hossain. 2001. Growth and storability of Trichoderma harzianum and its effect on germination of tomato seeds. Bangladesh J. Seed Sci. & Tech. 5(1&2): 117-121.
  18. Welsh, J. and M. McClelland. 1990. Finger printing genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218. https://doi.org/10.1093/nar/18.24.7213
  19. Williams, J.G.K., J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535. https://doi.org/10.1093/nar/18.22.6531
  20. Yang, X. and C.F. Quiros. 1995. Construction of a genetic linkage map in celery using DNA based markers, Genome. 38: 36-44. https://doi.org/10.1139/g95-005
  21. Zimand, G., L. Valinsky, Y. Elad, I Chet and S. Manulis. 1994. Use of the RAPD procedure for the identification of Trichoderma strains. Mycological Res. 98(5): 531-534. https://doi.org/10.1016/S0953-7562(09)80474-7

Cited by

  1. NativeTrichodermastrains isolated from Bangladesh with broad spectrum antifungal action against fungal phytopathogens vol.49, pp.1-4, 2016, https://doi.org/10.1080/03235408.2016.1147121