DOI QR코드

DOI QR Code

Independent Inheritance between df2 gene and ti gene in Soybean

  • Han, Eun-Hui (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) ;
  • Sung, Mi-Kyung (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) ;
  • Kim, Kyung-Roc (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) ;
  • Park, Jung-Soo (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) ;
  • Nam, Jin-Woo (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) ;
  • Chung, Jong-Il (Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University)
  • Received : 2010.08.27
  • Published : 2011.03.30

Abstract

Dwarfuess and Kunitz trypsin inhibitor (KTI) protein in soybean is useful traits for basic studies. df2 and ti gene control dwarfness and the expression of Kunitz trypsin inhibitor (KTI) protein in soybean, respectively. The objective of this research was to verify genetic linkage or independent inheritance of df2 and ti loci in soybean. The $F_2$ population was made by cross combination between "Gaechuck#2" (Df2Df2titi genotype, KTI protein absence and a normal growth type) and T210 (df2df2TiTi genotype, a dwarf growth type and KTI protein present). A total of 258 $F_2$ seeds were analyzed for the segregation of KTI protein using SDS-PAGE. And so, 198 $F_2$ plants were recorded for the segregation of dwarfness. The segregation ratio of 3 : 1 for Ti locus (201 Ti_ : 57 titi) and Df2 locus (143 Df2_ : 55 df2df2) was observed. Segregation ratio of 9 : 3 : 3 : 1 (116 Ti_Df2_: 44 Ti_df2df2: 27 titiDf2_: 11 titidf2df2) between df2 gene and ti gene was observed ($x^2$=3.53, P = 0.223). These results showed that df2 gene was inherited independently with the ti gene in soybean.

Keywords

References

  1. Byth, D. E. and C. R. Weber. 1969. Two mutant genes causing dwarfness in soybeans. J. Hered. 60: 278-280.
  2. Fehr, W. R. 1972. Inheritance of a mutation for dwarfness in soybeans. Crop Sci. 12: 212-213. https://doi.org/10.2135/cropsci1972.0011183X001200020019x
  3. Hidebrand, D. F., J. H. Orf and T. Hymowitz. 1980. Inheritance of an acid phosphatase and its linkage with the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Sci. 20: 83-85. https://doi.org/10.2135/cropsci1980.0011183X002000010019x
  4. Hymowitz, T. 1973. Electrophoretic analysis of SBTI-A2 in the USFA soybean germplasm collection. Crop Sci. 13: 420-421. https://doi.org/10.2135/cropsci1973.0011183X001300040008x
  5. Kiang, Y. T. 1987. Mapping three protein loci on a soybean chromosome. Crop Sci. 27: 44-46. https://doi.org/10.2135/cropsci1987.0011183X002700010011x
  6. Kim, M. S., M. J. Park, W. H. Jeong, K. C. Nam and J. I. Chung. 2006. SSR marker tightly linked to the Ti locus in soybean. Euphytica 152: 361-366. https://doi.org/10.1007/s10681-006-9223-3
  7. Kunitz, M. 1945 Crystallization of a soybean trypsin inhibitor from soybean. Science 101: 668-669. https://doi.org/10.1126/science.101.2635.668
  8. Lee, K. J., M. S. Park, M. K. Sung, M. S. Kim and J. I. Chung. 2008. Inheritance between Le gene and Ti gene in soybean (Glycine max L.). Korean J. Breed. Sci. 40(2): 97-100.
  9. Moraes, R. M. A., T. C. B. Soares, L. R. Colombo, M. F. S. Salla, J. G. A. Barros, N. D. Piovesan, E. G. Barros and M. A. Moreira. 2006. Assisted selection by specific DNA markers for genetic limination of the kunitz trypsin inhibitor and lectin in soybean seeds Euphytica 149: 221-226. https://doi.org/10.1007/s10681-005-9069-0
  10. Orf, J. H. and T. Hymowitz. 1979. Inheritance of the absence of the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Sci. 19: 107-109. https://doi.org/10.2135/cropsci1979.0011183X001900010026x
  11. Porter, K. B. and M. G. Weiss. 1948. The effect of polyploidy on soybeans. J. Am. Soc. Agron. 40: 710-724. https://doi.org/10.2134/agronj1948.00021962004000080005x
  12. Rackis, J. J., H. A. Sasame, R. R. Mann, R. L. Anderson and A. K. Smith. 1962. Soybean trypsin inhibitors: Isolation, purification and properties. Biochem. Biophys. 98: 471-478. https://doi.org/10.1016/0003-9861(62)90213-8
  13. Singh, L. C., M. Wilson and H. H. Hadley. 1969. Genetic differences in soybean trypsin inhibitor separated by disc electrophoresis. Crop Sci. 9: 489-491 https://doi.org/10.2135/cropsci1969.0011183X000900040031x
  14. Stewart, R. T. 1927. Dwarfs in soybeans. J. Hered. 18: 281-284.
  15. Sung, M. K., E. H. Han, K. R. Kim, J. S. Park, K. J. Hwang, J. W. Nam and J. I. Chung. 2010. Inheritance of Cgy1 gene and Ti gene in mature soybean seed. Korean J. Breed. Sci. 42(1): 35-39.
  16. Wang, K. J, Y. Takahata, K. Ito, Y. P. Zhao, K. I. Tsutsumi and N. Kaizuma. 2001. Genetic characterization of a novel soybean Kunitz trypsin inhibitor. Breed Sci. 51: 185-190. https://doi.org/10.1270/jsbbs.51.185
  17. Wang, K. J. and X. H. Li. 2005. Tif type of soybean Kunitz trypsin inhibitor exists in wild soybean of northern China. In: Proceedings of the 8th national soybean research conference of China, pp. 167-168.
  18. Weiss, M. G. 1970. Genetic linkage in soybeans: Linkage groups V and VI. Crop Sci. 10: 469-470. https://doi.org/10.2135/cropsci1970.0011183X001000050002x
  19. Westfall, R. L. and S. M. Hauge. 1948. The nutritive quality and the trypsin inhibitor content of soybean flour heated at various temperatures. J. Nutr. 35: 379-389.
  20. Woodworth, C. M. 1932. Genetics and breeding in the improvement of the soybean. Illinois Agr. Exp. Sta. Bull. 384: 297-404.
  21. Zhao, S. W. and H. Wang. 1992. A new electrophoretic variant of SBTi-A2 in soybean seed protein. Soybean Genet. Newsl. 19: 22-24.