Reproductive and Developmental Biology

  • 제35권1호
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  • Pages.55-60
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  • 2011
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향

Effect of Semen Collection Methods on the Post-thaw Viability of Cat Semen

  • 하아나 (경상대학교 농업생명과학대학 축산학과) ;
  • 윤진호 (경상대학교 농업생명과학대학 축산학과) ;
  • 김유곤 (경상대학교 농업생명과학대학 축산학과) ;
  • 조아라 (경상대학교 농업생명과학대학 축산학과) ;
  • 이경림 (경상대학교 농업생명과학대학 축산학과) ;
  • 공일근 (경상대학교 농업생명과학대학 축산학과)
  • Ha, A-Na (Department of Animal Science, Graduate School of Gyeongsang National University) ;
  • Yoon, Jin-Ho (Department of Animal Science, Graduate School of Gyeongsang National University) ;
  • Kim, Yu-Gon (Department of Animal Science, Graduate School of Gyeongsang National University) ;
  • Jo, A-Na (Department of Animal Science, Graduate School of Gyeongsang National University) ;
  • Lee, Kyeong-Rim (Department of Animal Science, Graduate School of Gyeongsang National University) ;
  • Kong, Il-Keun (Department of Animal Science, Graduate School of Gyeongsang National University)
  • 투고 : 2011.02.25
  • 심사 : 2011.03.03
  • 발행 : 2011.03.31
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초록

The objective of this study was carried out to evaluate the efficiency of sperm collection methods on the post-thaw viability of cat semen. The cat semen was collected by artificial virginal (AV) and electronic ejaculate (EE) methods. The composition of semen extender was consisted of Tris-buffer supplemented with 20% egg yolk and 1% P/S antibiotics in Ext I, and more added 8% glycerol, 1.0% Equex STM paste of total volume in Ext II. The collected semen was adjusted the concentration and then diluted in Ext I for optimal concentration. The diluted semen was cooling to $5^{\circ}C$ temperature in refrigerator for at least 2 hrs and then diluted stepwise with Ext II for at least 1 hrs. After an equilibration for 1 hrs, the cooled semen was packaged in 0.5 ml straw and then freezing on the $LN_2$ vapor over 5 cm above from $LN_2$ and then immersed directly in $LN_2$ for cryopreservation. The frozen semen was thawed in $38^{\circ}C$ water for 15 sec and then evaluated the motility, viability, and morphology. Post-thaw semen were calculated the motility by SMI (sperm motility index). The live-dead sperm was evaluated by Eosin-B and morphological evaluation was by Diff-quik kit staining. The post-thaw concentration ($89{\times}10^6$ /ml vs. $128{\times}10^6$ /ml), viability ($22.6{\pm}10.6%$ vs. $37.1{\pm}26.1%$), morphological normality ($27.0{\pm}50.2%$ vs. $45.6{\pm}123.0%$) of EE and AV groups were not significant different, but the post-thaw motility was significant lower in EE than that in AV group ($53.1{\pm}3.6$ vs. $73.6{\pm}5.7$) (p<0.05). In conclusion, semen collection methods did not significant different between EE and AV groups except of post-thaw motility and so both semen collection methods could be applied in feline semen collection methods.

키워드

참고문헌

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