환경생물 (Korean Journal of Environmental Biology)

  • 제29권4호
  • /
  • Pages.373-378
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  • 2011
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지

Comparison of Gene Mutation Frequency in $Tradescantia$ Stamen Hair Cells Detected after Chernobyl and Fukushima Nuclear Power Plant Accidents

  • Panek, Agnieszka (Institute of Nuclear Physics PAN, Department of Radiation and Environmental Biology) ;
  • Miszczyk, Justyna (Institute of Nuclear Physics PAN, Department of Radiation and Environmental Biology) ;
  • Kim, Jin-Kyu (Korea Atomic Energy Research Institute) ;
  • Cebulska-Wasilewska, Antonina (Institute of Nuclear Physics PAN, Department of Radiation and Environmental Biology)
  • 투고 : 2011.11.10
  • 심사 : 2011.11.17
  • 발행 : 2011.11.30
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초록

Our aim was to investigate the genotoxicity of ambient air in the Krak$\acute{o}$w area after Fukushima Nuclear Power Plant (NPP) accident and compare with results from Chernobyl fallout. For the detection of ambient air genotoxicity the technique for screening gene mutation frequency in somatic cells of the $Tradescantia$ stamen hairs ($Trad$-SH assay) was used. Since 11th of March 2011 (Fukushima NPP accident), several pots containing at least 15 shoots of bioindicating plants were exposed to ambient air at 2 sites in the Krak$\acute{o}$w surrounding area, one in the city center, and about 100 pots in a control site (in the glasshouse of the Institute of Nuclear Physics) Continuous screening of mutations was performed. Progenies of 371,090 cells exposed were analyzed. Mutation frequency obtained in the first 10 days has shown a mean control level (GMF*100=$0.06{\pm}0.01$). At scoring period related to influence of a potential Fukushima fallout, a significant increase of gene mutation frequencies above the control level was observed at each site in the range, 0.10~0.33 depending on the location, (mean value for all sites GMF*100=$0.19{\pm}0.05$) that was associated with a strong expression of toxic effects. In the reported studies following the Chernobyl NPP accident monitoring $in$ $situ$ of the ambient air genotoxicity was performed in the period since April $29^{th}$ till June $3^{rd}$ 1986 also with Trad-SH bioindicator. In general, mutation frequency increases due to Chernobyl fallout(GMF*100=$0.43{\pm}0.02$) were corresponding to fluctuation of radioactivity in the air reported from physical measures, and to published reports about increase in chromosome aberration levels. Although, recent data obtained from monitoring of the ambient air quality in the Krak$\acute{o}$w and surroundings are lower when compared to results reported after Chernobyl NPP accident, though results express a significant increase above the control level and also are corresponding with increased air radioactivity reported from physical measurements. Statistically significant in comparison to control increase in gene mutation rates and more prolonged than that after Chernobyl fallout increase of GMF was observed during the period following the Fukushima NPP failure.

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참고문헌

  1. Cebulska-Wasilewska A, HP Leenhouts and KH Chadwick. 1981. Synergism between EMS and Xrays for the induction of somatic mutations in Tradescantia. Int. J. Radiat. Biol. 40:163-173. https://doi.org/10.1080/09553008114551041
  2. Cebulska-Wasilewska A. 1988. Detection of ambient air pollutants mutagenicity with Tradescantia stamen hair system. Nukleonika. 33:91-105.
  3. Cebulska-Wasilewska A. 1989. Comparison of ambient air mutagenicity detected with Tradescantia stamen hairs following Chernobyl accident year later. Environm. and Mol. Mutagenesis. 14 (Supp. 15):35.
  4. Cebulska-Wasilewska A and R Korzeniowski. 1989. Influence of some environmental factors on dose response curve for somatic mutations in Tradescantia. Environm. and Mol. Mutagenesis. 14 (Supp. 15):38.
  5. Celebuska-Wasilewska A. 1992. Trandescantia stamen hair mutation bioassay on the mutagenicity of radioisotope contaminated air following the Chernobyl nuclear accident and one year late. Mutat. Res. 270:23-29. https://doi.org/10.1016/0027-5107(92)90097-L
  6. Cebulska-Wasilewska A, K Schneider and JK Kim. 2001. Relative biological efficiency for the induction of various gene mutations in normal and enriched with 10B Tradescantia cells by neutrons from 252Cf source. Mut. Res. 474:57-70. https://doi.org/10.1016/S0027-5107(00)00157-3
  7. Do Santos TC, VR Crispim, Y Noualhetas, JF Macacini and HA Gomes. 2005. The study of the effects of low-level exposure to ionizing radiation using a bio-indicator system. Appl. Radiat. Isot. 62:313-316. https://doi.org/10.1016/j.apradiso.2004.08.049
  8. Ferreira MI, M Domingos, A Gomes Hde, PHN Saldiva and JV de Assuncao. 2007. Evaluation of mutagenic potential of contaminated atmosphere at Ibirapuera Park, Sao Paulo-SP, Brazil, using the Tradescantia stamen-hair assay. Environ. Pollut. 145:219-224. https://doi.org/10.1016/j.envpol.2006.03.013
  9. Ichikawa S, A Nakano, M Kenmochi, I Yamamoto, M Murai, E Takahashi, A Yamaguchi, K Watanabe, M Tomiyama, K Sugiyama, A Yogo, T Yazaki, M Okumura, N Shima, M Satoh, M Yoshimoto and LZ Xiao. 1996. Yearly variation of spontaneous somatic mutation frequency in the stamen hairs of Tradescantia clone KU 9 grown outdoors, which showed a significant increase after the Chernobyl accident. Mutat. Res. 349:249-259. https://doi.org/10.1016/0027-5107(95)00189-1
  10. Masson O, A Baeza, J Bieringer, K Brudecki, S Bucci, M Cappai, FP Carvalho, O Connan, C Cosma, A Dalheimer, D Didier, G Depuydt, LE De Geer, A De Vismes, L Gini, F Groppi, K Gudnason, R Gurriaran, D Hainz, O Halldorsson, D Hammond, O Hanley, K Holey, Z Homoki, A Ioannidou, K Isajenko, M Jankovick, C Katzlberger, M Kettunen, R Kierepko, R Kontro, PJM Kwakman, M Lecomte, L Leon Vintro, AP Leppanen, B Lind, G Lujaniene, P Mc Ginnity, C Mc Mahon, H Mala, S Manenti, M Manolopoulou, A Mattila, A Mauring, JW Mietelski, BS Møller, P Nielsen, J Nikolick, RMW Overwater, SE Palsson, C Papastefanou, I Penev, MK Pham, PP Povinec, H Rameback, MC Reis, W Ringer, A Rodriguez, P Rulik, PRJ Saey, V Samsonov, C Schlosser, G Sgorbati, BV Silobritiene, C Soderstrom, R Sogni, L Solier, M Sonck, G Steinhauser, T Steinkopff, P Steinmann, S Stoulos, I Sykora, D Todorovic, N Tooloutalaie, L Tositti, J Tschiersch, A Ugron, E Vagena, A Vargas, H Wershofen and O Zhukov. 2011. Tracking of airborne radionuclides from the damaged Fukushima Dai-Ichi nuclear reactor by European networks. Environ. Sci. Technol. 45:7670-7677. https://doi.org/10.1021/es2017158
  11. Minouflet M, S Ayrault, PM Badot, S Cotelle and JF Ferard. 2005. Assessment of the genotoxicity of 137Cs radiation using Vicia-micronucleus, Tradescantia-micronucleus and Tradescantia-stamen-hair mutation bioassays. J. Environ. Radioact. 81 (2-3):143-153. https://doi.org/10.1016/j.jenvrad.2005.01.003
  12. Underbrink AG, LH Schairer and AH Sparrow. 1973. Tradescantia stamen hairs: A radiobiological test system applicable to chemical mutagenesis. pp. 171-207. In Chemical Mutagens: Principles and Methods for their Detection (A. Hollaender ed.). Plenum Press. New York.