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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Effect of Lactobacillus plantarumCLP-1 on the Swine Viruses

Lactobacillus plantarumCLP-1이 돼지바이러스에 미치는 효과

  • Lee, Gun-Hee (Department of Biotechnology and Researcher, company of Cheilbio. LTD.) ;
  • Kim, Young-Hee (Department of Biotechnology and Researcher, company of Cheilbio. LTD.) ;
  • Cho, Hyeon-A (Department of Biotechnology and Researcher, company of Cheilbio. LTD.) ;
  • Kang, Sung-Gi (Department of Biotechnology and Researcher, company of Cheilbio. LTD.) ;
  • Kim, Dong-Gun (Department of Biotechnology and Researcher, company of Cheilbio. LTD.)
  • 이건희 (제일바이오 생명공학의 연구) ;
  • 김영희 (제일바이오 생명공학의 연구) ;
  • 조현아 (제일바이오 생명공학의 연구) ;
  • 강성기 (제일바이오 생명공학의 연구) ;
  • 김동건 (제일바이오 생명공학의 연구)
  • Received : 2010.12.10
  • Accepted : 2011.01.22
  • Published : 2011.02.28
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Abstract

To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-${\beta}$ activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.

Keywords

References

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