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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimized Production of Biosurfactant by the Indigenous Bacterium, Pseudoalteromonas sp. HK-3 Originating from Oil-Spilled Areas

유류누출 지역에서 유래한 토착세균, Pseudoalteromonas sp. HK-3 배양에서 생물계면활성제의 최적 생산

  • Cho, Su-Hee (Department of Biotechnology, Soonchunhyang University) ;
  • Ma, Chae-Woo (Department of Biotechnology, Soonchunhyang University) ;
  • Oh, Kye-Heon (Department of Biotechnology, Soonchunhyang University)
  • 조수희 (순천향대학교 생명공학과) ;
  • 마채우 (순천향대학교 해양생명공학과) ;
  • 오계헌 (순천향대학교 생명공학과)
  • Received : 2011.01.21
  • Accepted : 2011.02.23
  • Published : 2011.02.28
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Abstract

The principal objective of this study was to determine the optimal conditions for the production of biosurfactant by the indigenous bacterium, Pseudoalteromonas sp. HK-3, originating from oil-spilled areas. The relationship between total biosurfactant production and the factors affecting biosurfactant production were evaluated by statistical analysis using SPSS software. The effects of various supplemental carbon sources (e.g., glucose, dextrose, mannitol, citrate, acetate) on the maximal production of biosurfactant by the test culture of Pseudoalteromonas sp. HK-3 was then evaluated. As a result, mannitol was found in this study to be the best supplemental carbon source for the production of biosurfactant. A spot inoculation of crude cultural liquid containing the HK-3 cells generated the largest clear zone, whereas only small clear zones appeared around the spots inoculated with either supernatant only or cell pellets following centrifugation. Our results demonstrated that the HK-3 test culture supplemented with 2% mannitol at an initial pH of 6 generated the maximal amount of biosurfactant within 72 h of incubation.

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References

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