Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Association of Toll-Like Receptor 5 Gene Polymorphism with Susceptibility to Ossification of the Posterior Longitudinal Ligament of the Spine in Korean Population

  • Chung, Won-Suk (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Nam, Dong-Hyun (Department of Biofunctional Medicine and Diagnostics, College of Korean Medicine, Sang Ji University) ;
  • Jo, Dae-Jean (Department of Neurosurgery, School of Medicine, Kyung Hee University) ;
  • Lee, Jun-Hwan (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung Hee University)
  • Received : 2010.10.17
  • Accepted : 2011.01.07
  • Published : 2011.01.28
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Abstract

Objective: Ossification of the posterior longitudinal ligament (OPLL) has a strong genetic component. Specific gene polymorphisms may be associated with OPLL in several genes which regulate calcification in chondrocytes, change of extracellular collagen matrix and secretions of many growth factors and cytokines controlling bone morphogenesis. Toll-like receptor 5 (TLR5) may playa role in the pathogenesis of OPLL by intermediate nuclear factor-kappa B (NF-${\kappa}B$). The current study focused on coding single nucleotide polymorphisms (SNPs) of TLR5 for a case-control study investigating the relationship between TLR5 and OPLL in a Korean population. Methods: A total of 166 patients with OPLL and 231 controls were recruited for a case-control association study investigating the relationship between SNPs of TLR5 gene and OPLL. Four SNPs were genotyped by direct sequencing (rs5744168, rs5744169, rs2072493, and rs5744174). SNP data were analyzed using the SNPStats, SNPAnalyzer, Haploview, and Helixtree programs. Multiple logistic regression analysis with adjustment for age and gender was performed to calculate an odds ratio (OR). Results: None of SNPs were associated with OPLL in three alternative models (codominant, dominant, and recessive models; p> 0.05). A strong linkage disequilibrium block, including all 4 SNPs, was constructed using the Gabriel method. No haplotype was significantly associated with OPLL in three alternative models. Conclusion: These results suggest that Toll-like receptor 5 gene may not be associated with ossification of the posterior longitudinal ligament risk in Korean population.

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References

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