Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

$CD8^+$ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax

  • Kim, Hyo-Jin (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center) ;
  • Jung, Bong-Kwang (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center) ;
  • Lee, Jin-Joo (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center) ;
  • Pyo, Kyoung-Ho (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center) ;
  • Kim, Tae-Yun (Department of Environmental Medical Biology, Yonsei University College of Medicine) ;
  • Choi, Byung-Il (Department of Anatomy, College of Medicine, Korea University) ;
  • Kim, Tae-Woo (Divison of Infection and Immunology, Graduate School of Medicine, Korea University) ;
  • Hisaeda, Hajime (Department of Parasitology, Graduate School of Medicine, Gunma University) ;
  • Himeno, Kunisuke (Department of Parasitology, Graduate School of Medical Sciences, Kyushu University) ;
  • Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center)
  • Received : 2010.12.21
  • Accepted : 2011.02.03
  • Published : 2011.03.15
Download PDF
⟨ Previous Next ⟩

Abstract

Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8$^+$ T-cells and CD4$^+$ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8$^+$ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

Keywords

References

  1. Gurunathan S, Klinman DM, Seder RA. DNA vaccines: Immunology, application, and optimization. Annu Rev Immunol 2000; 18: 927-979. https://doi.org/10.1146/annurev.immunol.18.1.927
  2. Sakai T, Hisaeda H, Nakano Y, Zhang M, Takashima M, Ishii K, Maekawa Y, Matsumoto S, Nitta Y, Miyazaki J, Yamamoto S, Himeno K. Gene gun-based co-immunization of merozoite surface protein-1 cDNA with IL-12 expression plasmid confers protection against lethal Plasmodium yoelii in A/J mice. Vaccine 2003; 21: 1432-1444. https://doi.org/10.1016/S0264-410X(02)00665-5
  3. Rogers WO, Gowda K, Hoffman SL. Construction and immunogenicity of DNA vaccine plasmids encoding four Plasmodium vivax candidate vaccine antigens. Vaccine 1999; 17: 3136-3144. https://doi.org/10.1016/S0264-410X(99)00146-2
  4. Dobano C, Sedegah M, Rogers WO, Kumar S, Zheng H, Hoffman SL, Doolan D. Plasmodium: Mammalian codon optimization of malaria plasmid DNA vaccines enhances antibody responses but not T cell responses nor protective immunity. Exp Parasitol 2009; 122: 112-123. https://doi.org/10.1016/j.exppara.2009.02.010
  5. Remarque EJ, Faber BW, Kocken CHM, Thomas AW. Apical membrane antigen 1: A malaria vaccine candidate in review. Trends Parasitol 2008; 24: 74-84. https://doi.org/10.1016/j.pt.2007.12.002
  6. Kusi KA, Faber BW, Thomas AW, Remarque EJ. Humoral immune response to mixed PfAMA1 alleles; multivalent PfAMA1 vaccines induce broad specificity. PLOS One 2009; 4: e8110. https://doi.org/10.1371/journal.pone.0008110
  7. Dutta S, Sullivan JS, Grady KK, Haynes JD, Komisar J, Batchelor AH, Soisson L, Diggs CL, Heppner DG, Lanar DE, Collins WE, Barnwell JW. High antibody titer against apical membrane antigen-1 is required to protect against malaria in the Aotus model. PLOS One 2009; 4: e8138. https://doi.org/10.1371/journal.pone.0008138
  8. Kocken CHM, Dubbeld MA, Van Der Wel A, Pronk JT, Waters AP, Langermans JAM, Thomas AW. High-level expression of Plasmodium vivax apical membrane antigen 1 (AMA-1) in Pichia pastoris: Strong immunogenicity in Macaca mulatta immunized with P. vivax AMA-1 and adjuvant SBAS2. Infect Immun 1999; 67: 43-49.
  9. Bueno LL, Morais CG, Soares IDS, Bouillet LEM, Bruna-Romero O, Fontes CJ, Fujiwara RT, Braga EM. Plasmodium vivax recombinant vaccine candidate AMA-1 plays an important role in adaptive immune response eliciting differentiation of dendritic cells. Vaccine 2009; 27: 5581-5588. https://doi.org/10.1016/j.vaccine.2009.07.031
  10. Herrera S, Corradin G, Arevalo-Herrera M. An update on the search for a Plasmodium vivax vaccine. Trends Parasitol 2007; 23: 122-128. https://doi.org/10.1016/j.pt.2007.01.008
  11. World Health Organization. Update on development of vaccines against Plasmodium vivax malaria (http://www.who.int/vaccines-documents). 2005.
  12. Rodriguez MHC, Rodriguez KM, Oliveira TR, Comodo AN, Rodriguez MM, Kocken CHM, Thomas AW, Soares IS. Antibody response of naturally infected individuals to recombinant Plasmodium vivax apical membrane antigen-1. Int J Parasitol 2005; 35: 185-192. https://doi.org/10.1016/j.ijpara.2004.11.003
  13. Zhang M, Obata C, Hisaeda H, Ishii K, Murata S, Chiba T, Chiba T, Tanaka K, Li Y, Furue M, Chou B, Imai T, Duan X, Himeno K. A novel DNA vaccine based on ubiquitin-proteasome pathway targeting 'self'-antigens expressed in melanoma/melanocyte. Gene Therapy 2005; 12: 1049-1057. https://doi.org/10.1038/sj.gt.3302490
  14. Chai JY, Park YK, Guk SM, Oh KH, Oh MD, Lee SH, Kim HS, Wataya Y. trial for a DNA diagnosis of Plasmodium vivax malaria recently reemerging in the Republic of Korea using microtiter plate hybridization assay. Am J Trop Med Hyg 2000; 63(1-2): 80-84. https://doi.org/10.4269/ajtmh.2000.63.80
  15. Han ET, Park JH, Shin EH, Choi MH, Oh MD, Chai JY. Apical membrane antigen-1 (AMA-1) gene sequences of re-emerging Plasmodium vivax in South Korea. Korean J Parasitol 2002; 40: 157-162. https://doi.org/10.3347/kjp.2002.40.3.157
  16. Chung JY, Chun EH, Chun JH, Kho WG. Analysis of the Plasmodium vivax apical membrane antigen-1 gene from re-emerging Korean isolates. Parasitol Res 2003; 90: 325-329. https://doi.org/10.1007/s00436-002-0777-2
  17. Han ET, Song TE, Park JH, Shin EH, Guk SM, Kim TY, Chai JY. Allelic dimorphism in the merozoite surface protein-3alpha in Korean isolates of Plasmodium vivax. Am J Trop Med Hyg 2004; 71: 745-749.
  18. Pizarro JC, Vulliez-Le Normand B, Chesne-Seck ML, Collins CR, Withers-Martinez C, Hackett F, Blackman MJ, Faber BW, Remarque EJ, Kocken CHM, Thomas AW, Bentley GA. Crystal structure of the malaria vaccine candidate apical membrane antigen 1. Science 2005; 308: 408-411. https://doi.org/10.1126/science.1107449
  19. Moon SU, Na BK, Kang JM, Kim JY, Cho SH, Park YK, Sohn WM, Lin K, Kim TS. Genetic polymorphism and effect of natural selection at domain I of apical membrane antigen-1 (AMA-1) in Plasmodium vivax isolates from Myanmar. Acta Trop 2010; 114: 71-75. https://doi.org/10.1016/j.actatropica.2010.01.006
  20. Putaporntip C, Jongwutiwes S, Grynberg P, Cui L, Hughes AL. Nucleotide sequence polymorphism at the apical membrane antigen-1 locus reveals population history of Plasmodium vivax in Thailand. Infect Genet Evol 2009; 9: 1295-1300. https://doi.org/10.1016/j.meegid.2009.07.005
  21. Kloetzel PM. Antigen processing by the proteasome. Nat Rev Mol Cell Biol 2001; 2: 179-187. https://doi.org/10.1038/35056572
  22. Narum DL, Kumar S, Rogers WO, Fuhrmann SR, Liang H, Oakley M, Taye A, Sim BKL, Hoffman SL. Codon optimization of gene fragments encoding Plasmodium falciparum merozoite proteins enhances DNA vaccine protein expression and immunogenicity in mice. Infect Immun 2001; 69: 7250-7253. https://doi.org/10.1128/IAI.69.12.7250-7253.2001
  23. Yazdani SS, Shakri AR, Pattnaik P, Rizvi MMA, Chitnis CE. Improvement in yield and purity of a recombinant malaria vaccine candidate based on the receptor-binding domain of Plasmodium vivax Duffy binding protein by codon optimization. Biotechnol Lett 2006; 28: 1109-1114. https://doi.org/10.1007/s10529-006-9061-3
  24. Yoshida A, Nagata T, Uchijima M, Higashi T, Koide Y. Advantage of gene gun-mediated over intramuscular inoculation of plasmid DNA vaccine in reproducible induction of specific immune responses. Vaccine 2000; 18: 1725-1729. https://doi.org/10.1016/S0264-410X(99)00432-6
  25. Wang S, Zhang C, Zhang L, Li J, Huang Z, Lu S. The relative immunogenicity of DNA vaccines delivered by the intramuscular needle injection, electroporation and gene gun methods. Vaccine 2008; 26: 2100-2110. https://doi.org/10.1016/j.vaccine.2008.02.033