The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Chunghyuljihwang-tang on Phenylhydrazine-induced Hemolytic Anemia in Rats

청혈지황탕(淸血地黃湯)이 Phenylhydrazine으로 유발한 용혈성 빈혈 흰쥐에 미치는 영향

  • Yang, Hyeong-Ju (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Woo, Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 양형주 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실)
  • Received : 2010.12.08
  • Accepted : 2011.01.12
  • Published : 2011.01.30
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Abstract

Objectives: This study was performed in order to investigate the anti-hemolytic effect of Chunghyuljihwang-tang(CHJHT, Qingxuedihuang-tang) on phenylhydrazine-induced hemolytic anemia in Rats. Materials and Methods: Hemolytic anemia model of rats were induced by phenylhydrazine(PHZ, 40mg/kg/day, i.p.) for 3days. Male Sprague-Dawley rats were divided into three groups for their treatment. Normal group was injected normal saline for 3 days with distilled water(d.w.) orally, control group was injected PHZ for 3 days with d.w. and CHJHT group was injected PHZ for 3 days with administration of CHJHT for 10 days. RBC(Red blood cell), Hb(hemoglobin), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, reticulocyte were determined on day 0, 4, 7, 10. Body weight was investigated on day 0, 2, 4, 6, 8, 10 and the weight of spleen was measured on day 10. Results: For CHJHT group, the levels of RBC and Hb were significantly higher than control group on day 4, and the concentration of hemoglobin on day 7 was increased more than control group. Moreover, reticulocyte was significantly decreased on day 10 more than control group. These results could be concluded that CHJHT suppressed the hemolytic anemia by PHZ and improved hematological values of anemia earlier.

Keywords

References

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