The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Ojeok-san on the Maturation of Follicles and NGF Expression in Rats with Estradiol Valerate-induced Polycystic Ovaries

오적산(五積散)이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF발현에 미치는 영향

  • Kim, Jong-Dae (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Lee, Dong-Nyung (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Hyung-Jun (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University)
  • 김종대 (세명대학교 한의과대학 한방부인과교실) ;
  • 서일복 (세명대학교 한의과대학 한방부인과교실) ;
  • 이동녕 (세명대학교 한의과대학 한방부인과교실) ;
  • 김형준 (세명대학교 한의과대학 한방부인과교실)
  • Received : 2011.01.28
  • Accepted : 2011.02.09
  • Published : 2011.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study was designed to investigate the effect of Ojeok-san(OJS) on the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. OJS treated group(n=8) were injected with EV and orally administrated OJS for sixty days. Then, we measured weights of body, ovaries, adrenal glands and uterus of rats. The histomorphometrical changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(mg) of ovaries in OJS treated group($57.4{\pm}9.4$) were significantly increased(p < 0.05) compared with PCO control group($42.3{\pm}8.5$). - The numbers of mature follicles in OJS treated group($9.3{\pm}2.5$) were significantly increased(p < 0.05) compared with PCO control group($6.1{\pm}2.1$). - The expressions of NGF-immunoreactive cells in the ovarian granulosa cells in OJS treated group were weaker than PCO control group. Conclusion: From the these results, we concluded that Ojeok-san(OJS) has inhibitory effect on the development of EV-induced polycystic ovary. And it's effect maybe related with decreased NGF activities in the ovary.

Keywords

References

  1. 구병삼. 임상부인과 내분비학 제2판.서울:고려의학. 2001:181-7.
  2. Stein IF, Leventhal NL. Amenorrhoea associated with bilateral polycystic ovaries. Am J Obstet Gynecol. 1935;29:181-91. https://doi.org/10.1016/S0002-9378(15)30642-6
  3. 김탁. 다낭성 난소 증후군의 최신지견. 대한산부인과학회 연수강좌. 2002; 38-44.
  4. 최영길. 내분비학. 서울:의학출판사.1994:563-71.
  5. 김동일, 윤종원, 이태균. 다낭성 난소증후군에 관한 문헌적 고찰. 대한한방부인과학회지. 1997;10(1):73-91.
  6. 유영기, 서일복, 김형준, 이동녕. 腎氣丸合蒼附導痰湯이 Estradiol Valerate 로 유도된 흰쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2007; 20(1):84-98.
  7. 남은정, 이동녕, 서일복, 김형준. 定經湯이 Estradiol Valerate로 유도된 흰 쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2007;20(1):99-113.
  8. 김희주, 김윤상, 임은미. 蒼附六君湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2008;21(2):1-16.
  9. 이연경, 서일복, 이동녕, 김형준. 歸脾湯이 Estradiol Valerate로 유도된 흰 쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2008;21(3):60-74.
  10. 윤문희, 이동녕, 서일복, 김형준. 開鬱二陳湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소 발달에 미치는 영향. 대한한방부인과학회지. 2010; 23(2):1-19.
  11. 구희준, 조성희. 皂角刺가 estradiol valerate로 유발된 백서의 다낭성 난소 에 미치는 영향. 대한한방부인과학회지. 2010;23(2):71-84.
  12. 신선미, 임현정, 이정은, 유동열. 다낭성난소증후군을 동반한 원발성 불임 환자 1례의 임상보고. 대전대학교 한의학연구소 논문집. 2008;17(1):173-80.
  13. 윤소영, 강명자. 다낭성 난소증후군 환자의 한방 치료에 대한 임상적 고찰. 대한한방부인과학회지. 2000;13(2):437-51.
  14. 陳貴延, 楊思澍. 實用中西醫結合診斷治療學. 北京:中國醫藥科技出版社. 1991 :1004-9.
  15. 吳桂芳, 李祥云. 中醫藥治療多囊卵巢綜合症硏究槪况. 河南中醫. 1994;14(2) :123-5.
  16. 周羽珍. 蔡小蓀治療婦科痼疾驗方拾萃. 江蘇中醫. 1996;17(1):9-10.
  17. 金維新. 不孕症的診斷與中醫治療. 北京:科學出版社. 1992:172-7.
  18. 강순수. 바른 方劑學. 서울:대성문화사. 1996:260-1.
  19. 陳師文. 太平惠民和劑局方(卷2). 台北:旋風出版社. 1953:2-3.
  20. Brawer JR, Munoz M, Farookhi R. Development of the polycystic ovarian condition(PCO) in the estradiol valerate -treated rat. Biol Reprod. 1986;35 :647-55. https://doi.org/10.1095/biolreprod35.3.647
  21. Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril. 2004;81:19-25.
  22. 許浚. 東醫寶鑑. 서울:여강출판사. 2001 :1479.
  23. 黃度淵. 證脈.方藥合編. 서울:남산당. 1992:136-7.
  24. Elisabet SV et al.. Rats with steroidinduced polycystic ovaries develop hypertension and increased sympathetic nervous system activity. Reproductive Biology and Endocrinology. 2005;3:44. https://doi.org/10.1186/1477-7827-3-44
  25. Lara HE et al.. Activation of ovarian sympathetic nerves in polycystic ovary syndrome. Endocrinology. 1993;133(6) :2690-5. https://doi.org/10.1210/en.133.6.2690
  26. Levi-Montalcini R. The nerve growth factor 35 years later. Science. 1987; 237(4819):1154-62. https://doi.org/10.1126/science.3306916
  27. Dissen GA et al. A role for trkA nerve growth factor receptors in mammalian ovulation. Endocrinology. 1996;137(1) :198-209. https://doi.org/10.1210/en.137.1.198
  28. Dissen GA et al. Intraovarian excess of nerve growth factor increase androgen secretion and disrupts estrous cyclicity in the rat. Endocrinology. 2000;141(3) :1073-81. https://doi.org/10.1210/en.141.3.1073
  29. Dyer CA, Erickson GF. Norepinephrine amplifies human chorionic gonadotropin -stimulated androgen biosynthesis by ovarian theca-intestitial cells. Endocrinology. 1985;116(4):1645-52. https://doi.org/10.1210/endo-116-4-1645
  30. 대한내분비학회. 내분비학. 서울:고려의학. 1999:622-3.
  31. Vale W et al. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science. 1981;213 :1394-7. https://doi.org/10.1126/science.6267699
  32. Smagin GN, Heinrichs SC, Dunn AJ. The role of CRH in behavioral responses to stress. Peptides. 2001; 22:713-24. https://doi.org/10.1016/S0196-9781(01)00384-9

Cited by

  1. 혈부축어탕(血府逐瘀湯)이 Estradiol Valerate로 유발된 흰쥐의 다낭성 난소 및 수태에 미치는 영향 vol.25, pp.2, 2011, https://doi.org/10.15204/jkobgy.2012.25.2.043
  2. 반하(半夏)가 다낭성 난소 증후군이 유발된 흰쥐의 난소 조직에 미치는 영향 vol.25, pp.2, 2011, https://doi.org/10.15204/jkobgy.2012.25.2.066
  3. LPS유도 염증 동물모델에서 오적산의 항염증 효과 vol.42, pp.2, 2011, https://doi.org/10.13048/jkm.21012