Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Detection of Protein Molecules by Electrical Current Response Using Two-Electrode Method

  • Lyu, Hong-Kun (Green Energy Research Division, Daegu-Gyeongbuk Institute of Science and Technology(DGIST)) ;
  • Woo, Sung-Ho (Green Energy Research Division, Daegu-Gyeongbuk Institute of Science and Technology(DGIST)) ;
  • Han, Yoon-Soo (Department of Advanced Energy Material Science and Engineering, Catholic University of Daegu) ;
  • Lee, Hee-Ho (School of Electrical Engineering and Computer Science, Kyungpook National University) ;
  • Shin, Jang-Kyoo (School of Electrical Engineering and Computer Science, Kyungpook National University)
  • Received : 2010.12.30
  • Accepted : 2011.03.16
  • Published : 2011.03.31
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Abstract

In order to protect human lives from disease, various biosensors having the potential to analyze a variety of biomolecules have been utilized. Biosensors constitute one of the most promising ways to monitor and detect various biomolecules corresponding to diseases. In this study, we demonstrate that the reaction of streptavidin molecules with biotin on a gold electrode can be detected using the twoelectrode method with a gold electrode and a platinum reference electrode. We also show the characteristics of the electrical current response. While detecting 2-${\mu}M$ streptavidin molecules dissolved in phosphate buffered saline(PBS) solution, we found that an analytical biosensor can operate on the principle of detecting an antigen-antibody reaction event of protein molecules using the two-electrode method. We think that the "potential step" method might be useful to detect the occurrence of any antigen-antibody reactions and can be combined with other devices or ICs such as BJTs, MOSFETs, and OP-amps for the detection of biomolecules of diseases.

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