The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Disruption of the Dual Specificity Kinase Gene Causes the Reduction of Virulence in Candida albicans

이중특이성 인산화 효소의 결손이 Candida albicans 병원성에 미치는 효과

  • Park, Yun-Hee (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Park, Hee-Moon (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • 박윤희 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 박희문 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Received : 2011.03.14
  • Accepted : 2011.03.21
  • Published : 2011.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The opportunistic human pathogen Candida albicans has the ability to convert from yeast-form to pseudohyphal or true hyphal form. The morphological transition is considered as an important virulence factor, because the decrease or lack in dimorphism causes the reduction of virulence. Our previous study revealed that the disruption of dual specificity kinase gene caused the reduction of dimorphism in C. albicans. Therefore we tested the effect of dual specificity kinase in virulence using mouse model. The mean survival time for kinase-defective strains was about 15 days in comparison with those of wild-type, 3.9 days. Moreover the fungal burden on kidneys for kinase-defective strains was decreased by ten-fold than that for wild-type. These results suggest possible involvement of dual specificity kinase in a novel signal transduction pathway for morphological transition and virulence of C. albicans.

C. albicans는 사람에게 병증을 유발하는 기회감염성 진균으로 효모형에서 균사형 및 위균사형으로 전환할 수 있다. 이러한 이형성의 감소나 상실은 병원성의 결여와 연관되어 있기에 C. albicans의 병원성에 가장 결정적인 요인으로 여겨진다. 선행 실험을 통하여 이중특이성 인산화 효소의 결손 균주에서 이형성의 감소를 관찰하였기에, 마우스 모델에서 병원성에 미치는 효과를 확인하고자 하였다. 이중특이성 인산화 효소의 결손 균주를 감염시킨 경우, 마우스의 생존기간이 약 15일로 야생형의 3.9일보다 증가하였다. 또한 신장조직에 침투한 C. albicans의 세포수는 야생형에 비해 10배 가량 감소하였다. 본 연구는 C. albicans에서 이형성과 병원성에 연관된 이중특이성 인산화효소에 의한 새로운 신호전달기작의 가능성을 제시한다.

Keywords

References

  1. Alonso-Monge, R., Navarro-Garcia, F., Molero, G., Diez-Orejas, R., Gustin, M., Pla, J., Sanchez, M., and Nombela, C. 1999. Role of the mitogen-activated protein kinase Hog1p in morphogenesis and virulence of Candida albicans. J. Bacteriol. 181:3058-3068.
  2. Biswas, S., Van Dijck, P., and Datta, A. 2007. Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71:348-376. https://doi.org/10.1128/MMBR.00009-06
  3. Calderone, R. A. and Fonzi, W. A. 2001. Virulence factors of Candida albicans. Trends Microbiol. 9:327-335. https://doi.org/10.1016/S0966-842X(01)02094-7
  4. Dhillon, N. K., Sharma, S., and Khuller, G. K. 2003. Signaling through protein kinases and transcriptional regulators in Candida albicans. Crit. Rev. Microbiol. 29:259-275. https://doi.org/10.1080/713610451
  5. Lorenz, M. C. and Fink, G. R. 2001. The glyoxylate cycle is required for fungal virulence. Nature. 412:83-86. https://doi.org/10.1038/35083594
  6. Odds, F. C., Webster, C. E., Mayuranathan, P., and Simmons, P. D. 1988. Candida concentrations in the vagina and their association with signs and symptoms of vaginal candidosis. J. Med. Vet. Mycol. 26:277-283. https://doi.org/10.1080/02681218880000391
  7. Park, Y. H. 2008. Functional analyses of LAMMER kinase in budding yeast. Ph. D. Thesis. Chungnam National University, Daejeon, Korea.
  8. Sudbery, P., Gow, N., and Berman, J. 2004. The distinct morphogenic states of Candida albicans. Trends Microbiol. 12:317-324. https://doi.org/10.1016/j.tim.2004.05.008

Cited by

  1. Plant quarantine isolated cultivation system in Korea and results of recorded in 2005-2012 vol.40, pp.4, 2013, https://doi.org/10.7744/cnujas.2013.40.4.281