한국균학회지 (The Korean Journal of Mycology)

  • 제39권1호
  • /
  • Pages.7-10
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  • 2011
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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울릉도의 자생란과 공생하는 난균근균의 분자생물학적 동정

Molecular Identification of Orchid Mycorrhizal Fungi of Native Orchids in Ulleung Island

  • 염재영 (한국교원대학교 생물교육과) ;
  • 정재민 (국립수목원 산림자원보존과) ;
  • 이병천 (국립수목원 산림자원보존과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Youm, Jae-Young (Department of Biology Education, Korea National University of Education) ;
  • Chung, Jae-Min (Department of Forest Resources Conservation, Korea National Arboretum) ;
  • Lee, Byung-Chun (Department of Forest Resources Conservation, Korea National Arboretum) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 투고 : 2010.12.10
  • 심사 : 2011.03.07
  • 발행 : 2011.04.01
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초록

울릉도에서 채집된 6종의 지생란의 뿌리에서 난균근균을 확인하였다. 난균근균의 감염이 확인되었다. 각 종의 뿌리로 부터 분리된 난균근균이 분자적인 분석을 통하여 확인되였다. 뿌리로부터 분리된 균에서 DNA를 추출하여, 담자균류에 특이적인 프라이머인 ITS1-OF와 ITS4-OF를 사용하여 ITS 지역을 증폭한 후 염기서열을 분석하여 두 식물의 뿌리에 공생하는 난균근균을 동정하였다. 분자적인 분석을 통해 Tulasnellaceae 와 Ceratobasidaceae에 속하는 난균근균이 확인되었다.

Orchid mycorrhizal fungi (OMF) were examined in roots of the six terrestrial species of orchids collected in Ulleung Islands. Seven OMF isolates from the roots of orchids were identified based on morphological and molecular characters. Internal transcribed spacer region of OMF DNA was amplified using basidiomycete-specific ITS primers, ITS1-OF and ITS4-OF. OMF beloning to Tulasnellaceae and Ceratobasidaceae was identified through molecular analysis.

키워드

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피인용 문헌

  1. Undescribed Fungal Species Found in Ulleungdo, Korea vol.43, pp.1, 2015, https://doi.org/10.4489/KJM.2015.43.1.53
  2. Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea vol.40, pp.3, 2012, https://doi.org/10.4489/KJM.2012.40.3.132
  3. Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea vol.41, pp.1, 2013, https://doi.org/10.4489/KJM.2013.41.1.9