Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Protective Effect of Chlorogenic Acid against Aβ-Induced Neurotoxicity

  • Lee, Chan-Woo (Immune Modulation Lab., College of Pharmacy, Research Institute for Translational System Biomics, Chung-Ang University) ;
  • Won, Tae-Joon (Immune Modulation Lab., College of Pharmacy, Research Institute for Translational System Biomics, Chung-Ang University) ;
  • Kim, Hak-Rim (Department of Pharmacology, College of Medicine, Dankook University) ;
  • Lee, Dong-Ho (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Hwang, Kwang-Woo (Immune Modulation Lab., College of Pharmacy, Research Institute for Translational System Biomics, Chung-Ang University) ;
  • Park, So-Young (Department of Pharmacognosy, College of Pharmacy, Dankook University)
  • Received : 2011.01.08
  • Accepted : 2011.02.17
  • Published : 2011.04.30
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Abstract

Beta-amyloid (A${\beta}$) is considered as one of the major causes of Alzheimer's disease. This study examined the neuroprotective effects of chlorogenic acid, a naturally occurring polyphenol which is distributed widely in plants, fruits and vegetables, against A${\beta}$-induced toxicity. A${\beta}$ decreased significantly the viability of PC12 cells. This was accompanied by an increase in the intracellular calcium levels and cleaved caspase-3. In addition, A${\beta}$ induced an increase in Bax, and a decrease in Bcl-2 compared to the controls. However, a pre-treatment with chlorogenic acid rescued the PC12 cells from A${\beta}$ by attenuating the elevated intracellular calcium levels and reducing the levels of the apoptosis related proteins, including caspase-3, Bcl-2 and Bax. These results suggest that the protective effects of chlorogenic acid are, at least in parts, by attenuating the intracellular calcium influx and reducing apoptosis induced by A${\beta}$.

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References

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