Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of 17α-Methyltestosterone on Growth and Induced Sex Change in Longtooth Grouper Epinephelus bruneus (Bloch)

  • Park, In-Seok (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Im, Soo-Yeon (Mechanical and Environment Research Division, Korea Marine Equipment Reseach Institute)
  • Received : 2010.08.10
  • Accepted : 2011.03.14
  • Published : 2011.03.31
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Abstract

We examined the effects of 17${\alpha}$-methyltestosterone (MT) on growth and induced sex change in the longtooth grouper Epinephelus bruneus. The growth rate for body weight (GRW) and specific growth rate (SGR) of the group injected with MT over 8 weeks were significantly higher than those of the sham-injected control group, the group injected over 4 weeks, and the control group. Of the orally administrated groups, the GRWs of the control group and the group given 1 kg of feed with 2.0 mg of MT were highest and did not significantly differ from one another. For SGR, the treatment groups differed significantly, and the SGR of the control group was higher than those of the other groups (P<0.05). The condition factor (CF) of the group injected over 4 weeks was higher than those injected over 8 weeks, the sham-injected control group over 8 weeks, and the control group. The CF did not significantly differ between the sham-injected control group over 8 weeks and the injected group over 8 weeks, but these two groups differed from the control group (P<0.05). The CFs of the groups given 1 kg of feed with 0.5, 1.0, and 2.0 mg of MT were significantly higher than that of the control group (P<0.05). The feed efficiency ratio was not significantly affected by MT administration. Most of the experimental groups receiving MT developed many unidentified germ cell cysts and perinucleolus oocytes, although neither spermazoa cells nor sex-changed males were observed in any of the treatments.

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References

  1. Beardmore JA, Mair GC and Lewis RI. 2001. Monosex male production in finfish as exemplified by tilapia: applications, problems, and prospects. Aquaculture197, 283-301. https://doi.org/10.1016/S0044-8486(01)00590-7
  2. Brusle-Sicard S, Debas L, Fourcault B and Fuchs J. 1992. Ultrastructural study of sex inversion in a protogynous hermaphrodite, Epinephelus microdon (Teleostei, Serranidae). Reprod Nutr Dev 32, 393-406. https://doi.org/10.1051/rnd:19920409
  3. Chao TM and Lim LC. 1991. Recent developments in the breeding of grouper (Epinephelus spp) in Singapore. Singapore J Primary Ind 19, 78-93.
  4. Chen CP, Hsieh HL and Chang KH. 1980. Some aspects of the sex change and reproductive biology of the grouper, Epinephelus diacanthus (Cuvier et Valenciensis). Bull Inst Bot Acad Sin 19, 11-17.
  5. Chen FY, Chow M, Chao TM and Lim R. 1977. Artificial spawning and larval rearing of the grouper, Epinephelus twain (FORSKAL) in Singapore. Singapore J Primary Ind 5, 1-21.
  6. Duncan DB. 1955. Multiple-range and multiple F tests. Biometrics 1, 1-42.
  7. Hirose K and Hibiya T. 1968. Physiological studies on growth-promoting effect of protein-anabolic steroids on fish. I. Effects on goldfish. Bull Jap Soc Sci Fish 34, 466-472. https://doi.org/10.2331/suisan.34.466
  8. Howerton RD, Okimoto DK and Grau EG. 1992. The effect of orally aiministered $17{\alpha}$-methyltestosterone and triiodothyronine on growth and proximate body composition of seawater-adapted tilapia (Oreochromis mossambicus). Aquacult Fish Manage 23, 123-128.
  9. Hunter G and Donaldson EM. 1983. Hormonal sex control and its application to fish culture. In: Hoar WS, Randall DJ, Donaldson EM (eds) Fish Physiology.Academic Press, Orlando, Florida, 223-303.
  10. Kim IS and Lee WO. 1994. Fish fauna from Cheju Island, Korea. The Study Group of Korean Fish Fauna Department of Biology, Chonbuk National University,1-51.
  11. Kuwaye TT, Oimoto DK, Shimoda SK, Howerton RD, Lm H-R, Pang PKT and Grau EG. 1993. Effect of $17{\alpha}$-methyltestosterone on the growth of the euryhaline tilapia, Oreochromis mossambicus, in fresh water and in seawater. Aquaculture 113, 137-152. https://doi.org/10.1016/0044-8486(93)90347-2
  12. Lee Y-D, Kim H-B, Song C-B, Rho S and Lee J-J. 1996. Hormonal induction of sex reversal in serranid fish, Epinephelus septemfasciatus. J Aquaculture 9, 19-23.
  13. McBride JR and Fagerlund UHM. 1973. The use of $17{\alpha}$-methyltestosterone for promoting weight increases in juvenile pacific salmon. J Fish Res Board Can 30, 1099-1104. https://doi.org/10.1139/f73-180
  14. Pandian TJ and Sheela SG. 1995. Hormonal induction of sex reversal in fish. Aquaculture 138, 1-22. https://doi.org/10.1016/0044-8486(95)01075-0
  15. Park MO and Park I-S. 2009. Long-term effects of passive integrated transponder (PIT) tagging on the kelp grouper Epinephelus bruneus. J Fish Biol 74, 285-288. https://doi.org/10.1111/j.1095-8649.2008.02115.x
  16. Park MO, Hur WJ, Im S-Y, Seol D-W, Lee JW and Park I-S. 2008. Anesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus. Aquacult Res 39, 877-884. https://doi.org/10.1111/j.1365-2109.2008.01941.x
  17. Piferrer F. 2001. Endocrine sex control strategies for the ferminization of teleost fish. Aquaculture 197, 229-281. https://doi.org/10.1016/S0044-8486(01)00589-0
  18. Sadovy Y and Colin PL. 1995. Sexual development and sexuality in the Nassau grouper. J Fish Biol 46, 961-976. https://doi.org/10.1111/j.1095-8649.1995.tb01401.x
  19. Sarter K, Papadaki M, Zanuy S and Mylonas CC. 2006. Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release $17{\alpha}$-methyltestosterone implants. Aquaculture 256, 443-456. https://doi.org/10.1016/j.aquaculture.2006.01.034
  20. Shapiro DY. 1987. Differentiation and evolution of sex change in fishes. Bioscience 37, 490-496. https://doi.org/10.2307/1310421
  21. Shapiro DY, Sadovy Y and McGehee MA. 1993. Periodicity of sex change and reproduction in the rod hind, Epinephelus guttatus, a protogynous grouper. Bull Mar Sci 53, 1151-1162.
  22. Smith CL. 1965. The patterns of sexuality and the classification of serranid fishes. American Museum Novitates 2207, 1-20.
  23. Tan SM and Tan KS. 1974. Biology of the tropical grouper, Epinephelus tauvina (Forskal). I: A preliminary study on hermaphroditism in E. tauvina. Singap. J Primary Ind 2, 123-133.
  24. Tan-Fermin JD, Garcia LMB and Castillo AR Jr. 1994. Induction of sex inversion on juvenile grouper, Epinephelus suillus, (Valenciennes) by injections $17{\alpha}$-methyltestosterone. Jap J Ichthyol 40, 413-420.
  25. Yeh S-L, Dai Q-C, Chu Y-T, Kuo C-M, Ting Y-Y and Chang C-F. 2003a. Induced sex changed, spawning and larviculture of potato grouper, Epinephelus tukula. Aquaculture 228, 371-381. https://doi.org/10.1016/S0044-8486(03)00316-8
  26. Yeh S-L, Kuo C-M, Thing Y-Y and Chang C-F. 2003b. Androgens stimulate sex change in protogynous grouper, Epinephelus coiodes: spawning performancein sex-changed males. Comp Biochem Physiol C 135, 375-382.
  27. Yeh S-L, Thing Y-Y and Kuo C-M. 1988. Induced sex reversal of grouper (Epinephelus salmonoides, Epinephelus fario) after implantation of pelleted androgen. Bull Taiwan Fish Res Inst 45, 103-114.
  28. Weatherley AH and Gill HS. 1987. The biology of fish growth. In: Weatherley AH, Gill HS (eds) Influence of Hormones. Academic Press, Orlando, Florida, 177-208.