Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Expression Pattern of Antioxidant Enzymes Genes in the Ventral Prostates of Rats Exposed to Procymidone and/or Testosterone after Castration

  • Lee, Jong-Geol (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yon, Jung-Min (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jung, Ki-Youn (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lin, Chunmei (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jung, A-Young (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Beom-Jun (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yun, Young-Won (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University) ;
  • Nam, Sang-Yoon (Department of Veterinary Medicine, College of Veterinary Medicine and Research, Institute of Veterinary Medicine, Chungbuk National University)
  • Received : 2011.11.30
  • Accepted : 2011.12.19
  • Published : 2011.12.31
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Abstract

Procymidone is a fungicide with anti-androgenic properties widely used to protect fruits from fungal infection, which induces an excessive reactive oxygen species production in male reproductive organs. In this study, to clarify whether procymidone affect the cellular antioxidant system of prostate at onset of puberty, gene expression patterns of the representative antioxidant enzymes such as cytoplasmic glutathione peroxidase (GPx1), phospholipid hydroperoxide GPx (PHGPx), selenoprotein P (SePP), cytoplasmic copper/zinc superoxide dismutase (SOD1), and manganese SOD (SOD2) were investigated in the rat ventral prostates exposed to procymidone using real-time RT-PCR analyses. Seven-week-old Sprague-Dawley rats castrated at 6 weeks old were treated with procymidone (25, 50, or 100 mg/kg per day) orally for 7 consecutive days after testosterone propionate (0.4 mg/kg per day) administration by subcutaneous injection. As compared to normal control animals, GPx1 mRNA expression in prostates significantly increased by the administration with TP and/or procymidone. However, PHGPx and SOD1 mRNA levels significanatly decreased by over 25 mg/kg of procymidone treatment and SePP and SOD2 mRNA levels was significanatly reduced by over 50 mg/kg of procymidone treatment. These findings indicate that procymidone may affect the antioxidant system of prostatic cells in up-regulation mode of GPx1, but in down-regulation modes of PHGPx, SePP, SOD1, and SOD2, suggesting that procymidone may affect differently the cellular antioxidant system of prostate according to the exposure doses.

Keywords

References

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