한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제26권4호
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  • Pages.257-263
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  • 2011
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

Protective Effects of Silymarin against the Toxicity of Bisphenol A (BPA) on Boar Sperm Quality

  • Jang, Hyun-Young (College of Animal Life Science, Kangwon National University) ;
  • Kong, Hong-Sik (Dept. of Biotechnology, Genomic Engineering Center, Hankyong National University) ;
  • Choi, Byoung-Yang (College of Animal Life Science, Kangwon National University) ;
  • Shin, Jong-Suh (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Jong-Tack (School of Veterinary Medicine, Kangwon National University) ;
  • Park, In-Chul (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
  • 투고 : 2011.11.30
  • 심사 : 2011.12.27
  • 발행 : 2011.12.31
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초록

BPA, a diphenyl compound containing groups, that make it structurally similar to synthetic estrogen and is considered as one of the major endocrine disruptors. Silymarin has extensively been used to prevent and/or alleviate some human disease, especially for the treatment of adverse liver conditions. It has an antioxidative efficacy and cancer preventive efficacy. Therefore, we examined the hypothesis that silymarin can inhibit BPA-induced toxicity in boar sperm duing in vitro storage. Sperm characteristics (motility, viability, membrane integrity and mitochondrion activity) in semen exposed to BPA (10~200 uM) were sharply lowered, while it increase in a dose and time dependent manner due to silymarin addition (50~200 uM) into semen extender in the presence of BPA (100 uM). All of the evaluated characteristics were gradually improved in the groups that were treated with silymarin (50~200 uM) in the presence of BPA (100 uM) in comparison to BPA 100 uM alone group, irrespective of incubation periods (3 and 6 h). These results demonstrate that silymarin can ameliorate the toxicity of BPA on boar sperm characteristics during in vitro storage, suggesting that silymarin indirectly act as an antioxidant.

키워드

참고문헌

  1. Abdollahi M, Ranjbar A, Shadnia S, Nikfar S and Rezale A. 2004. Pesticides and oxidative stress: a review. Med. Sci. Monit. 10:141-147.
  2. Aitken RJ, Ryan Al, Baker MA and Mclaughlin EA. 2004. Redox activity associated with the maturation and capacitation of mammalian spermatozoa. Free Radic. Biol Med. 36:994-1010. https://doi.org/10.1016/j.freeradbiomed.2004.01.017
  3. Al-Hiyasat AS, Darmani H and Elbetieha AM. 2004. Leached components from dental composites and their effects on fertility of female mice. Eur. J. Oral. Sci. 1129:165-173.
  4. Brotons JA, Olea-Serrano MF, Villalobos M, Pedraza V and Olea N. 1995. Xenoestrogens released from lacquer coatings in food cans. Environ. Health Perspect. 103:608-612. https://doi.org/10.1289/ehp.95103608
  5. Cerolini S, Maldjian A, Surai P and Noble R. 2000. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci. 58:99-111. https://doi.org/10.1016/S0378-4320(99)00035-4
  6. Colerangle JB and Roy D. 1997. Profound effects of the weak environmental estrogen-like chemical bisphenol A on the growth of the mammary gland of Noble rats. J. Steroid Biochem. Mol. Biol. 60:153-160. https://doi.org/10.1016/S0960-0760(96)00130-6
  7. Falk, F. J, A. J. Ricci and M. S. Wolf. 1992. Pesticides and polychlorinated biphenyl residues in human breast lipid and their relation to breest cancer. Arch. Environ. Health. Perspect. 47:143-146.
  8. Fraschini F, Demartini G and Esposti D. 2002. Pharmacology of silymarin. Clin. Drug Invest. 22:51-65. https://doi.org/10.2165/00044011-200222010-00007
  9. Fraser L, Dziekonska A, Strezek R and Stezezek J. 2007. Dialysis of boar semen prior to freezing-thawing: Its effects on post-thaw sperm chacteristics. Theriogenology 67:994-1003. https://doi.org/10.1016/j.theriogenology.2006.12.002
  10. Funahashi H and Sano T. 2005. Select antioxidants improve the function of extended boar semen stored at 10 degrees C. Theriogenology 63:1605-1616. https://doi.org/10.1016/j.theriogenology.2004.06.016
  11. Game C, Gagnon MM, Webb D and Lim R. 2006. Endocrine disruption in male mosquitofish (Gambusia holbrooki) inhabiting wetlands in Western Australia. Ecotoxicology 15:665-672. https://doi.org/10.1007/s10646-006-0104-2
  12. Gazak R, Walterova D and Kren V. 2007. Silybin and silymarin: new and emerging applications in medicine. Curr. Med. Chem. 14:315-338. https://doi.org/10.2174/092986707779941159
  13. Halliwall B and Gutteridge JMC. 1989. Lipid peroxidation: a radical chain reaction. In: Free Radicals in Biology and Medicine. 2nd ed., Oxford, UK, Oxford University press. 188-276.
  14. Jang HY, Lee HY, Cheong HT, Kim JT, Park IC, Park CH and Yang BK. 2010. Development of sperm MTT assay for its application in boar semen. J. Emb. Trans. 25:229- 235.
  15. Janin A, Coudert L, Riche P, Mercier G, Cooper P and Blais JF. 2011. Application of a CCA-treated wood waste decontamination process to other copper-based preservativetreated wood after disposal. J. Hazard Mater. 186:1880-1887. https://doi.org/10.1016/j.jhazmat.2010.12.094
  16. Jou MJ, Peng TI, Reiter RJ, Jou SB, Wu HY and Wen ST, 2004. Visualization of the antioxidative effects of melatonin at the mitochondrial level during oxidative stressinduced apoptosis of rat brain astrocytes. J. Pineal. Res. 37:55-70. https://doi.org/10.1111/j.1600-079X.2004.00140.x
  17. Jung HY, Kim YH, Kim BW, Park IC, Cheong HT, Kim JT, Park Ck, Kong HS, Lee HK and Yang BK. 2010. Ameliorative effects of melatonin against hydrogen peroxide-induced oxidative stress on boar sperm characteristics and subsequent in vitro embryo development. Reprod. Dom. Anim. 45:943-950. https://doi.org/10.1111/j.1439-0531.2009.01466.x
  18. Katiyar SK, Roy AM and Baliga MS. 2005. Silymarin induces apoptosis primarily through a p53-dependent pathway involving Bcl-2/Bax, cytochrome c release, and caspase activation. Mol. Cancer Ther. 4:207-216. https://doi.org/10.4161/cbt.4.2.1442
  19. Kaur M, Agarwal R. 2007. Silymarin and epithelial cancer chemoprevention:How close we are to beside? Toxicol. Applied Phamacol. 225:350-359.
  20. Krishnan KA, Proudman JA, Bolt DJ and Bahr JM. 1993. Development of an homologous radioimmunoassay for chicken follicle-stimulating hormone and measurement of plasma FSH during the ovulatory cycle. Comp. Biochem. Physiol. Comp. Physiol. 105:729-734. https://doi.org/10.1016/0300-9629(93)90275-9
  21. Kumaresan A, Kadirvel G, Bujarbaruah KM, Bardoloi RK, Das A, Kumar S and Naskar S. 2009. Preservation of boar semen at 18${^{\circ}C}$ induces lipid peroxidation and apoptosis like changes in spermatozoa. Anim. Reprod. Sci. 110:162-171. https://doi.org/10.1016/j.anireprosci.2008.01.006
  22. Macro B, Cristina G, Franco G, Romano G, Monica S, Stefano S and Fabio B. 2001. Silybin, a new iron-chelating agent. J. Inorg. Biochem. 85:123-129. https://doi.org/10.1016/S0162-0134(01)00198-2
  23. Magure, H. C. 1998. Experimental photoallergic contant dermatits to bisphenol A. Acta. Derm. Venereol. 68:408-412.
  24. Manna SK, Mukhopadhyay A, Van NT and Affarwal BB. 1999. Silymarin suppresses TNF-induced activation of NF-Kappa B, c-Jun, N-terminal Kinase and apoptosis. J. Immunol. 163:6800-6809.
  25. Miligan SR, Balasubramian AV and Kalita JC. 1998. Relative potency of xonobiotic estrogens in an acute in vivo mammalian assay. Environ. Health Perspect. 106:23-26. https://doi.org/10.1289/ehp.9810623
  26. Papaconstantinou AD, Umbreit TH, Fisher BR, Goering PL, Lappas NT and Brown KM. 2000. Bisphenol A-induced increase in uterine weight and alterations in uterine morphology in ovariectomized B6C3F1 mice: role of the estrogen receptor. Toxicol. Sci. 56:332-339. https://doi.org/10.1093/toxsci/56.2.332
  27. Polyak SJ, Morishima C, Lohmann V, Pal S, Lee DY, Liu Y, Graf TN and Oberlies NH. 2010. Identification of hepatoprotective flavonolignans from silymarin. Proc. Nat. Acad. Sci. USA. 107:5995-5999. https://doi.org/10.1073/pnas.0914009107
  28. Sharpe RM. 2006. Pathways of endocrine disruption during male sexual differentiation and masculinisation. Best Pract. Res. Clin. Endocrinol. Metab. 20:91-110. https://doi.org/10.1016/j.beem.2005.09.005
  29. Siu AW, Maldonado M, Sanchez-Hidalgo M, Tan DX and Reiter RJ. 2006. Protective effects of melatonin in experimental free radical-related ocular diseases. J. Pineal. Res. 40:101-109. https://doi.org/10.1111/j.1600-079X.2005.00304.x
  30. Skottova N, Krecman V, Walterova D, Ulrichova J, Simanek V. 1997. Effect of silymarin and silybin on lipoprotein cholesterol levels and oxidizability of low density lipoprotein in rats. 11th International Symposium on Atheroschlerosis. pp134-139.
  31. Soose M. 1994. Properties of silibinin and of antioxidants against adrainmycin cytotoxicity in and unicellular eukaryote. Eur. J. Protistico. 30:394-403. https://doi.org/10.1016/S0932-4739(11)80214-2
  32. Steinmetz R, Michner NA, Grant A, Allen DL, Bigsby RM and Ben-Jonathan N. 1998. The xonoestrogen bisphenol A induce growth, differentiation, and c-fos gene expression in the female reproductive tract. Endocrinology 139:2741-2747. https://doi.org/10.1210/en.139.6.2741
  33. Stoker TE, Robinette CL, Britt BH, Laws SC and Cooper RL. 1999. Prepubertal exposure to compounds that increase prolactin secretion in the male rat: effects on the adult prostate. Biol. Reprod. 61:1636-1643. https://doi.org/10.1095/biolreprod61.6.1636
  34. Takao T, Nanamiya W, Nagano I, Asaba K, Kawabata K and Hashimoto K. 1999. Exposure with the environmental estrogen bisphenol A disrupts the male reproductive tract in young mice. Life Sci. 65:2351-2357. https://doi.org/10.1016/S0024-3205(99)00502-0
  35. Taylor CT. 2001. Antioxidants and reactive oxygen species in human fertility. Environ. Toxicol. Pharmacol. 10:189-198. https://doi.org/10.1016/S1382-6689(01)00099-0
  36. Toyama Y, Ohkawa M, Oku R, Maekawa M and Yuasa S. 2001. Neonatally administered diethylstilbestrol retards the development of the blood-testis barrier in the rat. J. Androl. 22:413-423.
  37. Valenzuela A, Aspillaga M, Vial S and Guerra R. 1989. Selectivity of silymarin on the increase of the glutathione content in different tissues of the rat. Planta. Med. 55:420-422. https://doi.org/10.1055/s-2006-962056
  38. Wagoner J, Negash A and Kane OJ. 2010. Multiple effects of silymarin on the hepatitis C virus life cycle. Hepatology 51:1912-1921. https://doi.org/10.1002/hep.23587
  39. Zeisel SH. 2004. Antioxidant suppress apoptosis. J. Nutr. 134:3179-3180. https://doi.org/10.1093/jn/134.11.3179S
  40. Zi X, Mukhtar H and Agarwal R. 1997. Novel cancer chemopreventive effects of a flavonoid antioxidant silymarin: inhibition of mRNA expression of an endogenous tumor promoter TNF alpha. Biochem. Biophys. Res. Commun. 239:334-9. https://doi.org/10.1006/bbrc.1997.7375