Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Preantral Follicle Isolation Technique on In Vitro Follicular Development in Mice

  • Lim, Hyun-Joo (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hoon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Sung-Jai (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Son, Jun-Kyu (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Baek, Kwang-Soo (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Kwon, Eung-Gi (Dairy Science Division, National Institute of Animal Science, RDA)
  • Received : 2011.08.03
  • Accepted : 2011.09.04
  • Published : 2011.12.31
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Abstract

The objective of this study was to compare of different isolation method of mouse preantral follicles, and to examine in vitro development of mouse preantral follicles isolated by different method. Preantral follicles were mechanically or enzymatically extracted from mouse ovaries. Mechanical isolation method used fine gauge needles and enzymatic method of isolating follicles used collagenase. The recovered preantral follicles were cultured for 10 days in alpha-minimal essential medium (${\alpha}$-MEM) + 5% FBS + Insulin-Transferrin-Selenium (ITS) + 100 mIU/ml FSH. The collected primary follicles by enzymatic treatment were higher than mechanical method. Others stage preantral follicle by mechanical isolation were higher than enzymatic method. After 10 days of culture, no statistical differences were shown in survival rates of preantral follicle among the 2 culture groups. The metaphase II rates of the oocytes were significantly higher (p<0.05) in mechanical method (17.8%) than in enzymatic method (5.1%). These results suggest that the isolation method of choice depends on the target stage preantral follicles and mechanical isolation is an optimal method of preantral folliclesin a culture of mouse preantral follicle.

Keywords

References

  1. Albertini DF, Combelles CM, Benecchi E and Carabatsos MJ. 2001. Cellular basis for paracrine regulation of ovarian follicle development. Reproduction 121:647-653. https://doi.org/10.1530/rep.0.1210647
  2. Betteridge KJ, Smith C, Stubbings RB, Xu KP and King WA. 1989. Potential genetic improvement of cattle by fertilization of fetal oocytes in vitro. J. Reprod. Fertil. Suppl. 38:87-98.
  3. Cortvrindt R, Smitz J and Van Steirteghem AC. 1996. In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Hum. Reprod. 11:2656-2666. https://doi.org/10.1093/oxfordjournals.humrep.a019188
  4. Driancourt MA. 1991. Follicular dynamics in sheep and cattle. Theriogenology 35:55-79. https://doi.org/10.1016/0093-691X(91)90148-7
  5. Eppig JJ and Downs SM. 1987. The effect of hypoxanthine on mouse oocyte growth and development in vitro: maintenance of meiotic arrest and gonadotrophin-induced oocyte maturation. Dev. Biol. 119:313-321. https://doi.org/10.1016/0012-1606(87)90037-6
  6. Eppig JJ and O'Brien MJ. 1996. Development in vitro of mouse oocytes from primordial follicles. Biol. Reprod. 54:197-207. https://doi.org/10.1095/biolreprod54.1.197
  7. Eppig JJ and Schroeder AC. 1989. Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro. Biol. Reprod. 41:268-276. https://doi.org/10.1095/biolreprod41.2.268
  8. Eppig JJ. 2001. Oocyte control of ovarian follicular development and function in mammals. Reproduction 122:829-838. https://doi.org/10.1530/rep.0.1220829
  9. Figueiredo JR, Hulshof SCJ, Hurk R. van den, Ectors FJ, Fontes RS, Nusgens B, Bevers MM and Beckers JF. 1993. Development of a combined new mechanical and enzymatic method for the isolation of intact preantral follicles from tetal, calf and adult bovine ovaries. Theriogenology 40:789-799. https://doi.org/10.1016/0093-691X(93)90214-P
  10. Gong SP and Lim JM. 2009. Effects of [beta]-mercaptoethanol on the growth of preantral follicles and the maturation of intrafollicular oocytes. Asian-Aust. J. Anim. Sci. 22:35-41. https://doi.org/10.5713/ajas.2009.80185
  11. Gosden RG, Boland NI, Spears N, Murray AA, Chapman M, Wade JC, Zohdy NI and Brown N. 1993.The biology and technology of follicular oocyte development in vitro. Reprod. Med. Rev. 2:129-152. https://doi.org/10.1017/S0962279900000685
  12. Gougeon A. 2003. The early stages of follicular growth. In: Trounson AO, Gosen RG, editors. Biology and Pathology of the Oocyte. Cambridge University Press, pp 29-43.
  13. Hartshorne GM. 1997. In vitro culture of ovarian follicles. J. Reprod. Ferti. 2:94-104.
  14. Johnston LA, Donoghue AM, O'Brien SJ and Wildt DE. 1991. Rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species. Biol. Reprod. 45:898-906. https://doi.org/10.1095/biolreprod45.6.898
  15. Ksiazkiewicz LK. 2006. Recent accievements in in vitro culture and preservation of ovarian follicles in mammals. Biol. Reprod. 6:3-16.
  16. Lim HJ, Kim DH, Im GS and Lim JM. 2010. Comparison of mechanical and enzymatic methods for the isolation of bovine ovarian follicles. J. Emb. Trans. 25:93-96.
  17. Lucci CM, Rumpf R, Figueiredo FR and Bao SN. 2002. Zebu (Bosindicus) ovarian preantral follicles: Morphological characterization and development of an efficient isolation method. Theriogenology 57:1467-1483. https://doi.org/10.1016/S0093-691X(02)00641-6
  18. Mariana JC and Nguyen HN. 1973. Folliculogenese chez la vache. Ann. Biol. Anim. Bioch. Biophys. 13:211-221. https://doi.org/10.1051/rnd:19730520
  19. Marion GB, Gier HB and Choudary JB. 1968. Micromorphology of the bovine ovarian follicular system. J. Anim. Sciences. 27:451-465.
  20. Nayudu PL and Osborn SM. 1992. Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J. Reprod. Fertil. 95:349-362. https://doi.org/10.1530/jrf.0.0950349
  21. Saumande J. 1981. Ovogeneseet folliculogenese. Rec. Med. Vet. 157:29-38.
  22. Skinner MK. 1990. Mesenchymal (stromal)-epithelial cell interactions in the testis and ovary which regulate gonadal function. Reprod. Fertil. Dev. 2:237-243. https://doi.org/10.1071/RD9900237
  23. Spears N, Boland NIMurray AA and Gosden RG. 1994. Mouse oocytes derived from in vitro growth primary ovarian follicles are fertile. Hum. Reprod. 9:527-532. https://doi.org/10.1093/oxfordjournals.humrep.a138539
  24. Telfer EE. 1996. The development of methods for isolation and culture of preantral follicles from bovine and porcine ovaries. Theriogenology 45:101-110. https://doi.org/10.1016/0093-691X(95)00359-G
  25. Webber LJ, Stubbs S, Stark J, Trew GH, Margar R, Hardy K and Franks S. 2003. Formation and early development of follicles in the polycystic ovary. Lancet. 362:1017-1021. https://doi.org/10.1016/S0140-6736(03)14410-8