Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Effect of hypoosmotic and thermal stress on gene expression and the activity of antioxidant enzymes in the cinnamon clownfish, Amphiprion melanopus

  • Park, Mi-Seon (East Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • Shin, Hyun-Suk (Division of Marine Environment & BioScience, Korea Maritime University) ;
  • Choi, Cheol-Young (Division of Marine Environment & BioScience, Korea Maritime University) ;
  • Kim, Na-Na (Division of Marine Environment & BioScience, Korea Maritime University) ;
  • Park, Dae-Won (Division of Electrical & Electronic Engineering, Korea Maritime University) ;
  • Kil, Gyung-Suk (Division of Electrical & Electronic Engineering, Korea Maritime University) ;
  • Lee, Je-Hee (Department of Marine Life Sciences, Jeju National University)
  • Received : 2010.12.12
  • Accepted : 2011.03.22
  • Published : 2011.09.30
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Abstract

We studied oxidative stress in cinnamon clownfish exposed to hypoosmotic (35 psu ${\rightarrow}$ 17.5 psu and 17.5 psu with prolactin (PRL)) and low temperature ($28^{\circ}C{\rightarrow}24^{\circ}C$ and $20^{\circ}C$) conditions by measuring the expression and activity of Cu/Zn-superoxide dismutase (Cu/Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX). The expression and activity of the antioxidant enzymes were significantly higher after the fish were exposed to $24^{\circ}C$, $20^{\circ}C$, and 17.5 psu, and expression was repressed by PRL treatment. Furthermore, we measured $H_2O_2$ and lipid peroxidation levels and found that they were significantly higher after exposure to the hypoosmotic and low-temperature environments. Additionally, we investigated changes in plasma AST and ALT levels after exposure to low temperature and hypoosmotic stress. These levels increased upon exposure of the clownfish to $24^{\circ}C$, $20^{\circ}C$, and 17.5 psu, but the levels of these parameters decreased in the 17.5 psu with PRL treatment during a salinity change. The results indicate that hypoosmotic and low-temperature conditions induce oxidative stress in cinnamon clownfish and that the parameters tested in this study may be indices of oxidative stress in the cinnamon clownfish.

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References

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