Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
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Noise-induced Stress Response on Cortisol, Glucose, albumin and Glucocorticoid Receptor Expression in the Japanese eel, Anguilla japonica

소음스트레스에 대한 뱀장어의 코티졸, 글루코스, 알부민과 Glucocorticoid Receptor 유전자 발현 연구

  • Park, Young-Chul (Inland Aquaculture Research Center, National Fisheries Research and Development Institute) ;
  • Kang, Yong-Jin (Inland Aquaculture Research Center, National Fisheries Research and Development Institute) ;
  • Jeon, Hyoung-Joo (Inland Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Han, Kyung-Nam (Dept. of Ocean Science, College of Natural Sciences, Inha University) ;
  • Baek, Jae-Min (Inland Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Lee, Wan-Ok (Inland Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Jin-Hyoung (Inland Fisheries Research Institute, National Fisheries Research and Development Institute)
  • Received : 2011.10.06
  • Accepted : 2011.11.22
  • Published : 2011.12.31
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Abstract

We measured blood plasma parameters(cortisol, glucose, albumin) and glucocorticoid receptor(GCR) gene expression level of the Japanese eel(Anguilla japonica) exposed to an explosion noise for an hour in order to evaluate the effects of noise stress and to explore the possibility of these parameters as biomarkers on noise stress for one of this valuable aquaculture species. Plasma cortisol and glucose reached high levels with significant differences compared to the control group, whereas albumin showed a low value after 1 h of exposure. In addition, tissue distribution of GCR gene expression was studied by real-time RT-PCR of ten organs(brain, eye, gill, gonad, heart, intestine, kidney, liver, muscle and skin). Liver showed the highest level of expression in the control followed by gill, muscle and intestine. A time-course study revealed induction in liver, gill, muscle and intestine after 30 min or 1 h of noise exposure.

소음 스트레스로 인한 뱀장어(Anguilla japonica)의 영향을 파악하기 위하여 스트레스 지표로 사용되는 코티졸, 포도당, 알부민 및 glucocorticoid receptor(GCR) 유전자의 발현 양을 측정, 분석하여 노출되지 않은 대조구와 비교하였다. 그 결과, 알부민은 노출 1시간 후에 낮은 값을 보인 반면 코티졸과 포도당은 대조구에 비해 매우 큰 차이를 보이며 높게 나타났다. GCR 유전자의 조직 발현 결과 간, 아가미, 근육 및 소장에서 많이 발현하였다. 소음 노출에 따른 시간의 변화에서 간과 아가미 근육과 소장에서 발현이 감소하는 양상을 나타내었다. 실험결과 뱀장어의 glucocorticoid receptor 유전자의 발현변화가 소음 스트레스로 인한 영향을 파악하는데 유용한 지표가 될 수 있음을 확인하였다.

Keywords

References

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