Effects of Amyda sinensis on Allergic Inflammation Mechanism related Atopy Dermatitis

별갑이 아토피 피부염에서의 알러지성 염증 반응에 미치는 영향

  • Sim, Tae-Kyung (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Ko, Dae-Kyoung (East-West Comparing Oriental Medicine Society) ;
  • Kim, Hyun-Chang (East-West Comparing Oriental Medicine Society) ;
  • Baek, Yeon-Jong (East-West Comparing Oriental Medicine Society) ;
  • Lee, Jae-Seok (East-West Comparing Oriental Medicine Society) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University)
  • Received : 2011.06.30
  • Accepted : 2011.08.17
  • Published : 2011.08.20

Abstract

Objectives: This study aimed to investigate the effects of Amyda sinensis (AS) on allergic inflammation mechanism related atopy dermatitis. Methods: To investigate the effects of AS, We study inhibitory effect of AS on the levels of pro-inflammatory cytokines released from Raw264.7 cell stimulated with LPS (lipopolysaccaride), and EoL-1, THP-1, Jutkat cell stimulated with DP (Dermatophagoides pteronyssinus), and LPS indused acute inflammatory BALB/c mouse model. Result: AS reduced the levels of IL-$1{\beta}$ released from Raw264.7 cell stimulated with LPS at 20 ug/ml, 5 ug/ml concentration, and reduced the levels of IL-6 in a dose-dependent. AS significantly reduced the levels of MCP-1 released from EoL-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at all the concentration, and significantly reduced the level of IL-8 at 0.1 ug/ml concentration. AS significantly reduced the levels of MCP-1 released from THP-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at 1 ug/ml concentration, and reduced the level of IL-6 in a dose-dependent. AS significantly reduced the levels of IL-4 released from Jutkat cell stimulated with DP at all the concentration, and significantly reduced the level of IL-5 at 0.1 ug/ml. 1 ug/ml concentration,. and reduced the level of TNF-${\alpha}$ in a dose-dependent. AS significantly reduced the levels of TNF-${\alpha}$, IL-6, IL-$1{\beta}$, in LPS indused acute inflammatory BALB/c mouse model, in a dose-dependent. Conclusion: These result suggested that AS has suppressive effect on pro-inflammatory cytokines in various cell lines through the regulation of immune system. AS could be applied on the medicinal sources for treatment of immune abnormal diseases such as atopy dermatitis afterward.

Keywords

References

  1. 대한피부과학회 교과서 편찬위원회 편저. 피부과학. 제5판. 서울, 여문각, p95, p170-174, 2008.
  2. Meagher LJ, Wines NY, Cooper AJ. Atopic dermatitis; review of immunopathogenesis and advances in immunosuppressive therapy. Australas J Dermatol 43(4):247-254, 2002. https://doi.org/10.1046/j.1440-0960.2002.00610.x
  3. Blaser K, Ring J, Capron. T Cell Regulation in Allergy, Asthma and Atopic Skin Diseases. S Karger Pub, p121-137, 2008.
  4. Fry L, Ellis CN. An Atlas of Aropic Eczema. Informa Healthcare, p87-101, 2003.
  5. 강란이, 박보경, 김선빈, 최학주, 김동희. 아토피피부염 동물 병태모델에서의 형개연교탕의 면역조절작용. 대전대학교 한의학연구소 논문집 18(2):47-62, 2009.
  6. 정승일, 최병민, 윤용갑, 이장원, 장선일. 천연물 유래 Th2 케모카인 억제제 발굴에 의한 새로운 아토피 피부염 치료기술 개발: 아토피 피부염 모델 NC/Nga 마우스에서 고삼 추출액의 억제 효과. 대한한의학방제학회지 17(1):141-151, 2009.
  7. 안종현, 김경준. 선퇴가 인간의 THP-1 단핵구에서 사이토카인 유전자 발현에 미치는 영향. 한방안이비인후피부과학회지 23(1):94-110, 2010.
  8. 박두병, 한재경, 김윤희. 양혈제습탕이 DNCB로 유도된 NC/Nga mice의 아토피피부염에 미치는 영향. 대한한방소아과학회지 23(3):263-291, 2009.
  9. 안상현, 김호현. 금은화의 NF-${\kappa}B$ 활성 억제를 통한 iNOS 조절이 NC/Nga 생쥐의 아토피 피부염에 미치는 영향. 동의생리병리학회지 24(2):278-283, 2010.
  10. Novak N, Bieber T, Leung DY. Immune mechanisms leading to atopic dermatitis. J Allergy Clin Immunol 112(6):128-139, 2003. https://doi.org/10.1016/j.jaci.2003.09.032
  11. Hamid Q, Boguniewicz M, and Leung DY. Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis, J Clin Invest 94(2):870-876, 1994. https://doi.org/10.1172/JCI117408
  12. Ono SJ, Nakamura T, Miyazaki D, Ohbayashi M, Dawson M, Toda M. Chemokines: roles in leukocyte development, trafficking, and effector function. J Allergy Clin Immunol 111(6):1185-1199, 2003. https://doi.org/10.1067/mai.2003.1594
  13. 가민 외. 중의피부과임상실천. 귀주과학출판사, p202, 2002.
  14. 범서강. 중서의결합치료피부병성병. 광동인민출판사, p188, 2005.
  15. 장지례. 장지례피부병임상경험집요. 중국의약과기출판사, p247-251, 2002.
  16. 서삼문. 중국피부병비방전서. 과학기술문헌출판사, p333-336, 2003.
  17. 피선명 외. 피부병중의치료여방제. 과학기술문헌출판사, p45-58, 1997.
  18. 노석선. 원색피부과학. (주)아이비씨기획, p499-501, 2006.
  19. 전국한의과대학 본초학교수 공편저. 본초학. 서울, 영림사, p601-603, 1991.
  20. 염정호. 니켈 및 코발트의 세포독성 기전에서 NO의 역할. 대한산업의학회지 13(3):274-277, 2001.
  21. Matsukawa A, Ohkawara S, Maeda T, Takagi K, Yoshinaga M. Production of IL-1 and IL-1 receptor antagonist and the pathological significance in lipopolysaccharide-induced arthritis. Clin Exp Immunol 93(2):206-211, 1993.
  22. Bomalaskim JS, Ford T, Hudson AP, Clark MA. Phospholipase A2-activating protein induces the synthesis of IL-1 and TNF in human monocyte. J Immunol 154(8):4027-4031, 1995.
  23. Noyori K, Okamoto R, Takagi T, Hyodo A, Suzuki K, Koshino T. Experimental induction of arthritis in rats immunized with Escherichia coli 0:14 lipopolysaccharide. J Rheumatol 21(3):484-488, 1994.
  24. Wong CK, Ho CY, Lam CW, Zhang JP, Hjelm NM. Differentiation of a human eosinophilic leukemic cell line, EoL-1: characterization by the expression of cytokine receptors, adhesion molecules, CD95 and eosinophilic cationic protein (ECP). Immunol Lett. 68(2-3):317-323, 1999.
  25. Mayumi M. EoL-1, a human eosinophilic cell line. Leuk Lymphoma 7(3):243-250, 1992. https://doi.org/10.3109/10428199209053629
  26. Sullivan TJ, Parker CW. Possible role of arachidonic acid and its metabolites in mediator release from rat mast cells. J Immunol 122(2):431-436, 1979.
  27. Auwerx J. The human leukemia cell line, THP-1: a multifaceted model for the study of monocyte - macrophage differentiation. Experientia 47(1):22-31, 1991. https://doi.org/10.1007/BF02041244
  28. Auwex J, Stael F, Ceuppens JL. Changes in IgG Fc receptor expression induced by phorbol 12-myristate 13-acetate treatment of THP-1 monocyte leukemia cells. Leuk Res. 16(3):317-327, 1992. https://doi.org/10.1016/0145-2126(92)90070-N
  29. 김병훈, 조재열. CD29 및 CD98 활성 매개에 의한 Jurkat T 세포의 유착과 그 활용. 약학회지 53(3):119-124, 2009.
  30. Singh VK, Mehrotra S, Agarwal SS. The paradigm of Th1 and Th2 cytokines: its relevance to autoimmunity and allergy. Immunol Res 20(2):147-161, 1990.