한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제30권4호
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  • Pages.390-394
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  • 2011
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
권호 표지
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Expression of Anti-breast Cancer Monoclonal Antibody in Transgenic Plant

  • Kim, Deuk-Su (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University) ;
  • Shao, Yingxue (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University) ;
  • Lee, Jeong-Hwan (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University) ;
  • Yoon, Joon-Sik (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University) ;
  • Park, Se-Ra (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University) ;
  • Choo, Young-Kug (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University) ;
  • Hwang, Kyung-A (Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Ko, Ki-Sung (Department of Biological Science, Institute of Biotechnology, College of Natural Sciences, Wonkwang University)
  • 투고 : 2011.11.11
  • 심사 : 2011.11.28
  • 발행 : 2011.12.31
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초록

BACKGROUND: Plant expression system for mass production of recombinant proteins has several advantages over other existing expression systems with economical and safety issues. Breast cancer is a cancer originating from breast tissue and comprises almost 25% of all cancers in women world widely. Lewis-Y antigen is difucosylated oligosaccharide and is carried by glycoconjugates at cancer cell surface. In this study, the anti-breast cancer mAb BR55, which recognizes the epitope Lewis-Y, was expressed in the plant expression system. METHODS AND RESULTS: We have developed plant system for production of mAb BR55 with or without KDEL (the ER retention signal). This ER retention signal was attached to C-terminus of protein to help retain the recombinant glycoprotein carrying oligomannose glycans and enhance glycoprotein accumulation. PCR analysis was performed and confirmed the presence of recombinant genes. Western blot validated that the recombinant proteins mAb BR55 with or without KDEL were expressed in transgenic plants, moreover, the expression level of the mAb BR55 with KDEL was higher compared to the mAb BR55 without KDEL. CONCLUSION: These results indicate that KDEL fusion is a good way to produce proteins and plant can be an ideal expression system to obtain proteins and enhance accumulation of proteins.

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참고문헌

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피인용 문헌

  1. Expression and function of plant-derived recombinant multiple monoclonal antibodies for the recognition of human colorectal cancer cells vol.9, pp.6, 2015, https://doi.org/10.1007/s11816-015-0373-4