한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제15권4호
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  • Pages.301-308
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  • 2011
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

결빙억제제의 종류, 농도 및 침지시간이 참전복 Haliotis discus hannai 발생배의 생존활성에 미치는 영향

Effects of Concentration and Immersion Time of Three Cryoprotective Agents on the Embryos Development of Abalone Haliotis discus hannai

  • 정종균 (부경대학교 해양바이오신소재학과) ;
  • 임한규 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 손맹현 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 김종현 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 정민환 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 장영진 (부경대학교 해양바이오신소재학과)
  • Chung, Jong-Kyun (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University) ;
  • Lim, Han-Kyu (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Son, Maeng-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Kim, Jong-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Jeong, Min-Hwan (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Chang, Young-Jin (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University)
  • 투고 : 2011.10.19
  • 심사 : 2011.11.13
  • 발행 : 2011.12.31
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초록

결빙억제제(CPA)의 종류, 농도 및 침지시간이 참전복 Haliotis discus hannai 발생배의 생존활성에 미치는 영향을 조사하였다. 4세포, 담륜자 및 피면자를 dimethyl sulfoxide, ethylene glycol, propylene glycol 농도별로 10분, 20분 및 30분간 침지하였다. CPA 침지 후 각 발생배의 발생진행률은 각 CPA 농도가 낮을수록, CPA 침지시간이 짧을수록 높았다. 또한, 피면자에서 비틀림전까지의 발생진행률은 4세포에서 담륜자까지의 것보다 높았다. CPA 침지 후 발생진행률 및 유생활성지수는 ethylene glycol에서 가장 높았다. 참전복 발생배의 냉동보존 효과를 높일 수 있는 CPA 및 농도는 ethylene glycol 2.0 M, 평형시간은 30분인 것으로 추정된다.

The tolerance evaluation for abalone Haliotis discus hannai embryos was performed using different concentrations of cryoprotective agents (CPAs): dimethyl sulfoxide, ethylene glycol and propylene glycol added to 0.2 M sucrose, respectively. 4-cell, trochophore and veliger were exposed in each CPA with different concentration for 10, 20 and 30 minutes of immersion time. Developmental rates were increased with decreased concentration of every CPA and decreased immersion time, and differed from types of CPA. Developmental rates of veliger in all the CPAs were higher than those of 4-cell and trochophore. The developmental rates and larval activity indices in ethylene glycol were comparatively higher than those in other CPAs and the effective CPA and its concentration for the cryopreservation of the abalone embryos was suggested as 2.0 M ethylene glycol with equilibration time of 30 minutes.

키워드

참고문헌

  1. Arii K, Suzuki T, Takai R, Kozima T (1992) Tolerance of fish eggs to dimethyl sulfoxide as the cryoprotectant. J Tokyo Univ Fish 79:121-126.
  2. Arii N, Nami K, Gomi F, Nakazawa T (1987) Cryoprotection of medaka embryos during development. Zool Sci 4:813-818.
  3. Billard R (1992) Reproduction in rainbow trout: Sex differentiation. dynamics of gametogenesis, biology and preservation of gametes. Aquaculture 100:263-298. https://doi.org/10.1016/0044-8486(92)90385-X
  4. Chang YJ, Kim YS, Choi YH, Lee JY (2001) Survival rates of frozen-thawed surf clam, Spisula sachalinensis larvae in five developmental stages. Dev Reprod 5:35-38.
  5. Chao NH, Lin TT, Chen YJ, Hsu HW, Liao IC (1997) Cryopreservation of late embryos and early larvae in the oyster and hard clam. Aquaculture 155:31-44. https://doi.org/10.1016/S0044-8486(97)00107-5
  6. Choi YH, Chang YJ (1999) Survival rates of trochophores from pearl oyster, Pinctada fucata martensii and Pacific oyster, Crassostrea gigas immersed in four kinds of cryoprotectant. J Kor Fish Soc 32:476-480.
  7. Choi YH, Jo PG, Kim T, Bai CB, Chang YJ (2007) The effects of cryopreservation on fine structures of pearl oyster (Pinctada fucata martensii) larvae. Dev Reprod 11:79-84.
  8. Cseh S, Corselli J, Nehlsin-Cannarella SL, Bailey LL, Szalay AA (1997) The effect of quick-freezing in ethylene glycol on morphological survival and in vitro development of mouse embryos frozen at different preimplantation stages. Theriogenology 48:43-50. https://doi.org/10.1016/S0093-691X(97)00190-8
  9. Jo PG, Choi YH, Kang KH, Kho KH, Go CS, Kim BH, Lim HK, Choi CY, Chang YJ (2002) Comparison of cryopreservation effects on D-shaped larvae of arkshell, Scapharca broughtonii by the kinds of additive and the concentrations of cryoprotectant. Kor J Malacol 18:77-82.
  10. Kasai M, Niwa K, Iritani A (1981) Effects of various cryoprotective agents on the survival of unfrozen mouse embryos. J Repord Fertil 63:175-180. https://doi.org/10.1530/jrf.0.0630175
  11. Lim HK (1998) Physiological properties of sperm and gamete preservation in black seabream, Acanthopagrus schlegeli. PhD Thesis, Pukyong National University Pusan Korea. pp. 130.
  12. Paniagua-Chavez CG, Tiersch TR (2001) Laboratory studies of cryopreservation of sperm and trochophore larvae of the Eastern oyster. Cryobiology 43:211-223. https://doi.org/10.1006/cryo.2001.2346
  13. Renard P, Cochard JC (1989) Effect of various cryoprotectants on Pacific oyster Crassostrea gigas Thunberg, Manila clam Ruditapes philippinarum Reeve and king scallop Pecten maximus (L.) embryos: Influence of the biochemical and osmotic effects. Cryo-Letters 10:169-180.
  14. Roux A, Sandenbergh L, Roodt-Wilding R (2008) Preliminary investigation to determine the cryotoxicity of various cryoprotectants on southern African abalone Haliotis midae embryos. Cryobiology 57:308-311. https://doi.org/10.1016/j.cryobiol.2008.09.009
  15. Suzuki T, Komada H, Takai R, Arii K, Kozima T (1995) Relation between toxicity of cryoprotectant DMSO and its concentration in several fish embryos. Fish Sci 61:193-197. https://doi.org/10.2331/suisan.61.193
  16. Zhang L, Liu X, Lu D, Chen S, Fang J (1992) Effects of several factors on the survival rate of fish embryo before it cryopreserved. Freshwat Fish Danshui Yuye 1:20-24.