Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antidiabetes and Angiotensin Converting Enzyme Inhibitory Activity of Sonchus asper (L) Hill Extract

큰방가지똥 추출물의 항당뇨 및 항고혈압효과

  • Xu, Ming-Lu (Henan Institute of Science and Technology) ;
  • Wang, Lan (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Xu, Gui-Fang (Henan Institute of Science and Technology) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
  • 허명록 (하남과학기술학원) ;
  • 왕란 (강원대학교 의생명과학대학) ;
  • 허계방 (하남과학기술학원) ;
  • 왕명현 (강원대학교 의생명과학대학)
  • Received : 2011.02.10
  • Accepted : 2011.03.11
  • Published : 2011.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we evaluated the bioactivities of methanol extract and its solvent fractions of Sonchus asper (L.) Hill. The EtOAc fraction of S. asper exhibited more strong antioxidant activity than other extracts as evidenced by the strongest 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity with a $EC_{50}$ value at $33.55\;{\mu}g/mL$ and reducing power, the total polyphenol (180.71 mg GAE/g) and flavonoid contents (145.86 mg QE/g) of S. asper extract were higher than other extracts. The EtOAc fraction of the S. asper also showed 47.38% mushroom tyrosinase inhibition activity, 56.22% ${\alpha}$-glucosidase inhibition and 46.58% ${\alpha}$-amylase inhibition ratio at 1 mg/mL. Both methylene chloride and EtOAc fractions of methanol extract of S. asper effectively reduced of the 86.34% and 62.03% angiotensin I converting enzyme (ACE) activity at 2 mg/mL, respectively. These findings suggest that the EtOAc fraction of the S. asper could be a potential antioxidant in food additive, medicinal, and industry product.

Keywords

References

  1. Korea national statistical office (2003) The cause of death statistics.
  2. Anoja, S. A. (2002) Antidianbetic effect of Panax ginseng Berry extract and the identification of an effective component. Diabets 51: 1858.
  3. Krall, M. A., Bradley A., L. P., Christlieb, R. F., Soell, A. R. and Joslins, J. S. (1985) Diabetes mellitus 12th ed., Lea & Febiger, Philadelphia.
  4. Cha, M. R., Park, J. H., Choi, Y. H., Choi, C. W., Hong, K. S., Choi, S. U., Kim, Y. S., Kim, Y. K., Kim, Y. H. and Ryu, S. Y. (2009) Alpha-glucosidase inhibitors from the branches extract of Cotinus coggygria. Kor. J. Pharmacogn. 40: 229-232.
  5. Bailey C. J. and Day, C. (1989) Traditional plant medicines as treatments for diabetes. Diabetic Care 12: 553-564. https://doi.org/10.2337/diacare.12.8.553
  6. Tsujimoto, T., Shioyama, E., Moriya, K., Kawaratani, H., Shirai, Y., Toyohara, M., Mitoro, A., Yamao, J., Fujii, H. and Fukui H. (2008) Pneumatosis cystoides intestinalis following alpha-glucosidase inhibitor treatment: a case report and review of the literature. World J. Gastroenterol. 14: 6087-6092. https://doi.org/10.3748/wjg.14.6087
  7. Koh, K. S. and Jeon, E. S. (2003) Ferns, fern-alles and seed bearing. plants of Korea. p. 726.
  8. Shimizu, S., Miyase, T., Ueno, A., and Usmanghani, K. (1989) Sesquiterpene lactone glycosides and ionone derivative glycosides from Sonchus asper. Phytochemistry 28: 3399-3402. https://doi.org/10.1016/0031-9422(89)80355-3
  9. Helal, A. M., Nakamura, N, El-Askary, H. and Hattori. M. (2000) Sesquiterpene lactone glucosides from Sonchus asper. Phytochemistry 53: 473-477. https://doi.org/10.1016/S0031-9422(99)00516-6
  10. Afolayan, A. J. and Jimoh, F. O. (2009) Nutritional quality of some wild leafy vegetables in South Africa. Int. J. Food Sci. Nutr. 60: 424-431. https://doi.org/10.1080/09637480701777928
  11. Khan, R. A., Khan, M. R., Sahreen, S. and Bokhari, J. (2010) Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat. Food Chem. Toxicol. 48: 2469-2476. https://doi.org/10.1016/j.fct.2010.06.016
  12. Chang, C. C., Yang, M. H., Wen, H. M. and Chern, J. C. (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10: 178-182.
  13. Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  14. Goh, E. J., Seong, E. S., Lee, J. G., Na, J. K., Lim, J. D., Kim, M. J., Kim, N. Y., Lee, G. H., Seo, J. S., Cheoi, D. S., Chung, I. M. and Yu, C. Y. (2009) Antioxidant ativities according to peelling and cultivated years of Astragalus membranaceus Roots. Kor. J. Med. Crop. Sci. 17: 233-237.
  15. Heo, B. G., Park, Y. S., Hou, W. N., Im, M. H., Park, Y. J., Kim, H. J., Sin, J. S. and Cho, J. Y. (2008) In vitro assay on physiological activities of flower and leaf extracts of red lotus. Kor. J. Hort. Sci. Technol. 26: 331-337.
  16. Hwang, I. G., Kim, H. Y., Shin, S. L., Lee, C. H., Lee, J. S., Jang, K. I. and Jeong, H. S. (2010) Biological activities of Coreopsis tinctoria Nutt. flower extracts. Kor. J. Hort. Sci. Technol. 28: 857-863.
  17. Gao, H. and Kawabata, J. (2005) $\alpha$-Glucoside inhibition of 6-hydroxyflavones. Part 3: Synthesis and evaluation of 2,3,4-trihydroxybenzoyl-containing flavonoid analogs and 6-aminoflavones as $\alpha-glucosidase$ inhibitors. Bioorg. Med. Chem. 13: 1661-1671. https://doi.org/10.1016/j.bmc.2004.12.010
  18. Cushman, D. W. and Cheung, H. S. (1971) Spectrophotometric assay and properties of the angiotensin I-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648. https://doi.org/10.1016/0006-2952(71)90292-9
  19. Bravo, L. (1998) Polyphenols: chemistry, dietary sources, methbolism, and nutritional significance. Nutr. Rev. 56: 317-333.
  20. Lee, K. D., Kim, J. S., Bae, J. O. and Yoon, H. S. (1992) Antioxidantive effectiveness of water extract and ether in wormwood (Artemisia Montana pampan). J. Kor. Soc. Food Nutr. 21: 17-22.
  21. Cha, J. Y., Kim, S. Y., Jeong, S. J. and Cho, Y. S. (1999) Effects of hesperetin and naringenin on lipid concentration in orotic acid treated mice. Kor. J. Life. Sci. 9: 389-394.
  22. Lee, K. D., Chang, H. K. and Kim, H. K. (1997) Antioxidative and nitrite scavenging activities of edible mushrooms. Kor. J. Food Sci. Technol. 29: 432-436.
  23. Gordon, M. H. (1990) The mechanism of antioxidant action in vitro. In Food antioxidants. Hudson BJF, ed. Elsevier Applied Science, London, UK. p. 1-8.
  24. Seo, J. K., Kang, M. J., Shin, J. H., Lee, S. J., Jeong, H. G., Sung, N. J. and Chung, Y. C. (2010) Antibacterial and antioxidant activities of solvent extracts from different parts of hagocho (Prunella vulgaris). J. Kor. Soc. Food Sci. Nutr. 39: 1425-1432. https://doi.org/10.3746/jkfn.2010.39.10.1425
  25. Pawelek, J. M. and Korner, A. M. (1982) The biosynthesis of mammalian melanin. Amer. Sci, 70: 136-141.
  26. Jung, S. W., Lee, M. K., Kim, S. J. and Han, D. S. (1995) Screening of tyrosinase inhibitor from plants. Kor. J. Food Sci. Technol. 27: 891-896.
  27. Ra, K. S., Suh, H. J., Chung, S. H. and Son, J. Y. (1997) Antioxidant activity of solvent extract from onion skin. Kor. J. Food Sci. Technol. 29: 595-600.
  28. Guo, J., Jin, Y. S., Han, W., Shim, T. H., Sa, J. H. and Wang, M. H. (2006) Studies for component analysis, antioxidative activity and $\alpha-glucosidase$ inhibitory activity from Equisetum arvense. J. Kor. Soc. Appl. Biol. Chem. 49: 77-81.
  29. Yin, Y., Heo, S. I., Jung, M. J. and Wang, M. H. (2009) Antioxidant and antidiabetic effects of various sections of Astragalus membranaceus. Kor. J. Pharmacogn. 40: 1-5.
  30. Park, Y. H., Lim, S. H., Ham, H. J., Jeong, H. N., Lee, K. J., Kim, K. H. and Kim, S. M. (2010) Comparison of biological activities of extracts from different Parts of Seabuckthorn (Hippophae rhamnoides L.). J. Kor. Soc. Food Sci. Nutr. 39: 975-979. https://doi.org/10.3746/jkfn.2010.39.7.975
  31. Kim, J. H., Kim, M. U. and Cho, Y. J. (2007) Isolation and identification of inhibitory compound from Crataegi fructus on ${\alpha}-amylase$ and $\alpha$-glucosidase. J. Kor. Soc. Appl. Biol. Chem. 50: 204-209.
  32. Ma, S. J. (2000) Inhibitory effect of onion seasoning on angiotensin converting enzyme. J. Kor. Soc. Food. Sci. Nutr. 29: 395-400.
  33. Kwon, Y. I., Vattem, D. A. and Shetty, K. (2006b). Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia. Pac. J. Clin. Nutr. 15: 107-118.
  34. Lee, S. E., Bang, J. G. and Seong, N. S. (2004) Inhibitory activity on angiotensin converting enzyme and antioxidant activity of Hovenia dulcis Thunb. Cortex extract. Kor. J. Med. Crop. Sci. 12: 79-84.