Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Selective 3,4-Dihydroxyphenylalanine Analysis in Human Urine as Ethoxycarbonyltert-butyldimethylsilyl Derivatives by Gas Chromatography-Mass Spectrometry

  • Paik, Man-Jeong (Department of Molecular Science and Technology, Ajou University) ;
  • Nguyen, Duc-Toan (Department of Molecular Science and Technology, Ajou University) ;
  • Yoon, Jae-Hwan (Biometabolite Analysis Laboratory, College of Pharmacy, Sungkyunkwan University) ;
  • Cho, In-Seon (Department of Molecular Science and Technology, Ajou University) ;
  • Shim, Woo-Young (Biometabolite Analysis Laboratory, College of Pharmacy, Sungkyunkwan University) ;
  • Kim, Kyoung-Rae (Department of Neurosurgery and Institute for Medical Science, School of Medicine, Ajou University) ;
  • Cho, Ki-Hong (Department of Molecular Science and Technology, Ajou University) ;
  • Choi, Sang-Dun (Department of Molecular Science and Technology, Ajou University) ;
  • Lee, Gwang (Institute for Medical Science, School of Medicine, Ajou University)
  • Received : 2010.09.15
  • Accepted : 2011.01.19
  • Published : 2011.03.20
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Abstract

A new analytical method for measurement of 3,4-dihydroxyphenylalanine (DOPA) in human urine was developed. DOPA from an aqueous solution was converted into an ethoxycarbonyl (EOC) derivative. A tertbutyldimethylsilyl (TBDMS) reaction under anhydrous conditions was then attempted for analysis by gas chromatography-mass spectrometry in selected ion monitoring mode. A new mass spectral data on DOPA as a tri-EOC/mono-TBDMS derivative was built. This method showed good linearity (r ${\geq}$ 0.999), precision (% relative standard deviation = 3.1-9.2), and accuracy (% relative error = -7.2-8.8), with a detection limit of 0.05 ng/mL. This selective and accurate method of DOPA analysis will be useful for biochemical monitoring of various neurological disorders including Parkinson's disease in biological fluids.

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