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  • 제11권1호
  • /
  • Pages.68-78
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  • 2011
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  • 1598-2629(pISSN)
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  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
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Effects of Pre-conditioning dose on the Immune Kinetics and Cytokine Production in the Leukocytes Infiltrating GVHD Tissues after MHC-matched Transplantation

  • Choi, Jung-Hwa (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Yoon, Hye-Won (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
  • Min, Chang-Ki (Department of Internal Medicine, The Catholic University of Korea) ;
  • Choi, Eun-Young (Department of Biomedical Sciences, Seoul National University College of Medicine)
  • 투고 : 2011.01.11
  • 심사 : 2011.01.28
  • 발행 : 2011.02.28
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초록

Background: Graft-versus-host disease (GVHD) is a huddle for success of hematopoietic stem cell transplantation. In this study, effects of irradiation dose on immune kinetics of GVHD were investigated using B6 ${\rightarrow}$ BALB.B system, a mouse model for GVHD after MHC-matched allogeneic transplantation. Methods: BALB.B mice were transplanted with bone marrow and spleen cells from C57BL/6 mice after irradiation with different doses. Leukocytes residing in the peripheral blood and target organs were collected periodically from the GVHD hosts for analysis of chimerism formation and immune kinetics along the GVHD development via flow cytometry. Myeloid cells were tested for production of IL-17 via flow cytometry. Results: Pre-conditioning of BALB.B hosts with 900 cGy and 400 cGy resulted in different chimerism of leukocytes from the blood and affected survival of GVHD hosts. Profiles of leukocytes infiltrating GVHD target organs, rather than profiles of peripheral blood leukocytes (PBLs), were significantly influenced by irradiation dose. Proportions of IL-17 producing cells in the infiltrating $Gr-1^+$ or $Mac-1^+$ cells were higher in the GVHD hosts with high does irradiation than those with low dose irradiation. Conclusion: Pre-conditioning dose affected tissue infiltration of leukocytes and cytokine production by myeloid cells in the target organs.

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참고문헌

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피인용 문헌

  1. A single-amino-acid variant of the H60 CD8 epitope generates specific immunity with diverse TCR recruitment vol.33, pp.4, 2012, https://doi.org/10.1007/s10059-012-0008-3
  2. In Vivo imaging of differences in early donor cell proliferation in graft-versus-host disease hosts with different pre-conditioning doses vol.33, pp.1, 2011, https://doi.org/10.1007/s10059-012-2228-y
  3. The Role of Chemokines in Mediating Graft Versus Host Disease: Opportunities for Novel Therapeutics vol.3, pp.None, 2012, https://doi.org/10.3389/fphar.2012.00023
  4. Nanocomposite Treatment Reduces Disease and Lethality in a Murine Model of Acute Graft-versus-Host Disease and Preserves Anti-Tumor Effects vol.10, pp.4, 2011, https://doi.org/10.1371/journal.pone.0123004