Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of major rice allergen and their clinical significance in children

  • Jeon, You-Hoon (Department of Pediatrics, Soonchunhyang University School of Medicine) ;
  • Oh, Se-Jo (Department of Pediatrics, Ajou University School of Medicine) ;
  • Yang, Hyeon-Jong (Department of Pediatrics, Soonchunhyang University School of Medicine) ;
  • Lee, Soo-Young (Department of Pediatrics, Ajou University School of Medicine) ;
  • Pyun, Bok-Yang (Department of Pediatrics, Soonchunhyang University School of Medicine)
  • Received : 2011.01.10
  • Accepted : 2011.09.23
  • Published : 2011.10.15
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Recently, an increase in the number of patients sensitized to rice allergen with or without clinical symptoms has been reported. This study was designed to determine the major allergens in rice and their clinical significance. Methods: Twenty-four children (15 boys and 9 girls; mean age, 16.3 months) with allergic disease, who were sensitized to rice antigen (by UniCAP) in the Pediatric Allergy Respiratory Center at Soonchunhyang University Hospital, were enrolled in this study. The allergenicity of various types of rice (raw, cooked, and heat-treated, simulated gastric fluid [SGF], and simulated intestinal fluid [SIF]) was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoglobulin E (IgE) immunoblots. The patients' medical records, including laboratory data and allergy symptoms after ingestion of rice were reviewed. Results: Patients were sensitized to an average of 13.5 food antigens and their mean total IgE was 6,888.7 kU/L. In SDS-PAGE, more than 16 protein bands were observed in the raw rice, whereas only 14-16 kDa and 31-35 kDa protein bands were observed in cooked rice. The common SDS-PAGE protein bands observed in SGF-, SIF-, and heat-treated rice were 9, 14, and 31 kDa. In a heated-rice IgE immunoblot, protein bands of 9, 14, and 31-33 kDa were found in 27.8%, 38.9%, and 38.9% of all sera, respectively, and in 50%, 50%, and 75%, of ser a from the 4 symptomatic patients, respectively. Conclusion: The 9-, 14-, and 31-kDa protein bands appeared to be the major allergens responsible for rice allergy symptoms.

Keywords

References

  1. Ikezawa Z, Miyakawa K, Komatsu H, Suga C, Miyakawa J, Sugiyama A, et al. A probable involvement of rice allergy in severe type of atopic dermatitis in Japan. Acta Derm Venereol Suppl (Stockh) 1992;176:103-107.
  2. Ikezawa Z, Ikebe T, Ogura H, Odajima H, Kurosaka F, Komatu H, et al. Mass trial of hypoallergenic rice (HRS-1) produced by enzymatic digestion in atopic dermatitis with suspected rice allergy. Acta Derm Venereol Suppl (Stockh) 1992;176:108-112.
  3. Nambu M, Shintaku N, Ohta S. Rice allergy. Pediatrics 2006;117:2331- 2332. https://doi.org/10.1542/peds.2006-0427
  4. Lee SY, Jeoung BJ, Kim DS, Kim KE, Lee KY. Detection of specific IgE and IgG4 to rice and its significance in atopic children. Pediatr Allergy Respir Dis 1993;3:74-82.
  5. Izumi H, Sugiyama M, Matsuda T, Nakamura R. Structural characterization of the 16-kDa allergen, RA17, in rice seeds. Prediction of the secondary structure and identification of intramolecular disulfide bridges. Biosci Biotechnol Biochem 1999;63:2059-2063. https://doi.org/10.1271/bbb.63.2059
  6. Ito M, Kato T, Matsuda T. Rice allergenic proteins, 14-16 kDa albumin and alpha-globulin, remain insoluble in rice grains recovered from rice miso (rice-containing fermented soybean paste). Biosci Biotechnol Biochem 2005;69:1137-1144. https://doi.org/10.1271/bbb.69.1137
  7. Usui Y, Nakase M, Hotta H, Urisu A, Aoki N, Kitajima K, et al. A 33-kDa allergen from rice (Oryza sativa L. Japonica). cDNA cloning, expression, and identification as a novel glyoxalase I. J Biol Chem 2001;276:11376-11381. https://doi.org/10.1074/jbc.M010337200
  8. Kumar R, Srivastava P, Kumari D, Fakhr H, Sridhara S, Arora N, et al. Rice (Oryza sativa) allergy in rhinitis and asthma patients: a clinico-immunological study. Immunobiology 2007;212:141-147. https://doi.org/10.1016/j.imbio.2006.11.006
  9. Arai T, Takaya T, Ito Y, Hayakawa K, Toshima S, Shibuya C, et al. Bronchial asthma induced by rice. Intern Med 1998;37:98-101. https://doi.org/10.2169/internalmedicine.37.98
  10. Radauer C, Breiteneder H. Evolutionary biology of plant food allergens. J Allergy Clin Immunol 2007;120:518-25. https://doi.org/10.1016/j.jaci.2007.07.024
  11. Asero R, Amato S, Alfieri B, Folloni S, Mistrello G. Rice: another potential cause of food allergy in patients sensitized to lipid transfer protein. Int Arch Allergy Immunol 2007;143:69-74. https://doi.org/10.1159/000098226
  12. Cheng HC, Cheng PT, Peng P, Lyu PC, Sun YJ. Lipid binding in rice nonspecific lipid transfer protein-1 complexes from Oryza sativa. Protein Sci 2004;13:2304-2315. https://doi.org/10.1110/ps.04799704
  13. Pastorello EA, Farioli L, Pravettoni V, Ispano M, Scibola E, Trambaioli C, et al. The maize major allergen, which is responsible for food-induced allergic reactions, is a lipid transfer protein. J Allergy Clin Immunol 2000; 106:744-751. https://doi.org/10.1067/mai.2000.108712
  14. Enrique E, Ahrazem O, Bartra J, Latorre MD, Castelló JV, de Mateo JA, et al. Lipid transfer protein is involved in rhinoconjunctivitis and asthma produced by rice inhalation. J Allergy Clin Immunol 2005;116:926-928. https://doi.org/10.1016/j.jaci.2005.05.041
  15. Asero R, Mistrello G, Roncarolo D, Amato S, Caldironi G, Barocci F, et al. Immunological cross-reactivity between lipid transfer proteins from botanically unrelated plant-derived foods: a clinical study. Allergy 2002; 57:900-906. https://doi.org/10.1034/j.1398-9995.2002.t01-1-23541.x
  16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685. https://doi.org/10.1038/227680a0
  17. Fu TJ, Abbott UR, Hatzos C. Digestibility of food allergens and nonallergenic proteins in simulated gastric fluid and simulated intestinal fluid-a comparative study. J Agric Food Chem 2002;50:7154-7160. https://doi.org/10.1021/jf020599h
  18. Vitoria JC, Camarero C, Sojo A, Ruiz A, Rodriguez-Soriano J. Enteropathy related to fish, rice, and chicken. Arch Dis Child 1982;57:44-48.
  19. Monzon S, Lombardero M, Perez-Camo I, Sáenz D, Lasanta J. Allergic rhinoconjunctivitis after ingestion of boiled rice. J Investig Allergol Clin Immunol 2008;18:487-488.
  20. Chun WS, Lee KS, Hong CH, Lee SY. A study on the cross-allergenicity between buckwheat and rice flour using IgE-immunoblot inhibition and ELISA-inhibition test. Pediatr Allergy Respir Dis 2000;10:161-170.
  21. Lee KY, Lee SY, Jeong BJ, Kim KE, Kim DS. Cross-reactivity among four cereals: rice, barley, wheat flour and buckwheat flour. Korean J Allergy 1993;13:65-74.
  22. Yum HY, Lee KE, Choi SY, Yang HS, Sohn MH, Lee SI, et al. Wild rice, hypoallergenic rice, and GMO rice-immuologic comparison. Pediatr Allergy Respir Dis 2005;15:117-125.
  23. Wu KG, Li TH, Chen CJ, Cheng HI, Wang TY. Correlations of serum Interleukin-16, total IgE, eosinophil cationic protein and total eosinophil counts with disease activity in children with atopic dermatitis. Int J Immunopathol Pharmacol 2011;24:15-23.
  24. Shin JE, Jeon YH, Yang HJ, Pyun BY. Comparison of clinical severity and laboratory results between atopic and non-atopic eczema in children. Pediatr Allergy Respir Dis 2008;18:219-227.
  25. Majamaa H, Laine S, Miettinen A. Eosinophil protein X and eosinophil cationic protein as indicators of intestinal inflammation in infants with atopic eczema and food allergy. Clin Exp Allergy 1999;29:1502-1506. https://doi.org/10.1046/j.1365-2222.1999.00666.x
  26. Figueredo E, Quirce S, del Amo A, Cuesta J, Arrieta I, Lahoz C, et al. Beerinduced anaphylaxis: identification of allergens. Allergy 1999;54:630-634. https://doi.org/10.1034/j.1398-9995.1999.00827.x
  27. Lehrer SB, Reese G, Malo JL, Lahoud C, Leong-Kee S, Goldberg B, et al. Corn allergens: IgE antibody reactivity and cross-reactivity with rice, soy, and peanut. Int Arch Allergy Immunol 1999;118:298-299. https://doi.org/10.1159/000024107
  28. Jones SM, Magnolfi CF, Cooke SK, Sampson HA. Immunologic crossreactivity among cereal grains and grasses in children with food hypersensitivity. J Allergy Clin Immunol 1995;96:341-351. https://doi.org/10.1016/S0091-6749(95)70053-6
  29. Yamada C, Izumi H, Hirano J, Mizukuchi A, Kise M, Matsuda T, et al. Degradation of soluble proteins including some allergens in brown rice grains by endogenous proteolytic activity during germination and heatprocessing. Biosci Biotechnol Biochem 2005;69:1877-1883. https://doi.org/10.1271/bbb.69.1877
  30. Yamada C, Yamashita Y, Seki R, Izumi H, Matsuda T, Kato Y. Digestion and gastrointestinal absorption of the 14-16-kDa rice allergens. Biosci Biotechnol Biochem 2006;70:1890-1897. https://doi.org/10.1271/bbb.60054
  31. Untersmayr E, Jensen-Jarolim E. The role of protein digestibility and antacids on food allergy outcomes. J Allergy Clin Immunol 2008;121:1301-1308. https://doi.org/10.1016/j.jaci.2008.04.025
  32. Kilshaw PJ, Heppell LM, Ford JE. Effects of heat treatment of cow's milk and whey on the nutritional quality and antigenic properties. Arch Dis Child 1982;57:842-847. https://doi.org/10.1136/adc.57.11.842
  33. Kim TH, Jung HH, Kim ES, Park JY, Kim KW, Sohn MH, et al. A case of rice allergy caused by thin rice porridge during the weaning period. Pediatr Allergy Respir Dis 2007;17:149-154.
  34. Katial RK, Grier TJ, Hazelhurst DM, Hershey J, Engler RJ. Deleterious effects of electron beam radiation on allergen extracts. J Allergy Clin Immunol 2002;110:215-219. https://doi.org/10.1067/mai.2002.126377

Cited by

  1. IgE mediated food allergy in Korean children: focused on plant food allergy vol.3, pp.1, 2011, https://doi.org/10.5415/apallergy.2013.3.1.15
  2. Identification of an Abundant 56 kDa Protein Implicated in Food Allergy as Granule-Bound Starch Synthase vol.61, pp.22, 2011, https://doi.org/10.1021/jf4014372
  3. 식품알레르기의 개요 vol.48, pp.1, 2015, https://doi.org/10.23093/fsi.2015.48.1.2
  4. Complementing the dietary fiber and antioxidant potential of gluten free bread with guava pulp powder vol.11, pp.4, 2011, https://doi.org/10.1007/s11694-017-9578-2
  5. Progress and challenges in improving the nutritional quality of rice (Oryza sativaL.) vol.57, pp.11, 2011, https://doi.org/10.1080/10408398.2015.1084992
  6. IgE-mediated food allergies in children: prevalence, triggers, and management vol.60, pp.4, 2011, https://doi.org/10.3345/kjp.2017.60.4.99