Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Expression of Exogenous Human Hepatic Nuclear Factor-$1{\alpha}$ by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2

  • Liao, Shunyao (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine) ;
  • Liu, Yunqiang (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine) ;
  • Zheng, Bing (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine) ;
  • Cho, Pyo-Yun (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine) ;
  • Song, Hyun-Ok (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine) ;
  • Lee, Yun-Seok (Department of Health Administration, Namseoul University) ;
  • Jung, Suk-Yul (Department of Biomedical Laboratory Science, Molecular Diagnosis Research Institute, Namseoul University) ;
  • Park, Hyun (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine)
  • Received : 2011.06.19
  • Accepted : 2011.09.01
  • Published : 2011.12.15
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Abstract

The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-$1{\alpha}$ is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-$1{\alpha}$ in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-$1{\alpha}$ expressed by a lentiviral vector (LV HNF-$1{\alpha}$) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-$1{\alpha}$ was observed to influence promoter activity, suggesting that host HNF-$1{\alpha}$ interacts with the Sub2 gene.

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References

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