Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Morphologic and Genetic Identification of Taenia Tapeworms in Tanzania and DNA Genotyping of Taenia solium

  • Eom, Kee-Seon S. (Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Yong, Tai-Soon (Department of Environmental Medical Biology & Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Min, Duk-Young (Department of Microbiology and Immunology, Euiji University College of Medicine) ;
  • Rim, Han-Jong (Department of Parasitology, Korea University College of Medicine) ;
  • Kihamia, Charles (Department of Parasitology, Muhimbili University College of Health Sciences) ;
  • Jeon, Hyeong-Kyu (Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine)
  • Received : 2011.04.04
  • Accepted : 2011.08.29
  • Published : 2011.12.15
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Abstract

Species identification of Taenia tapeworms was performed using morphologic observations and multiplex PCR and DNA sequencing of the mitochondrial cox1 gene. In 2008 and 2009, a total of 1,057 fecal samples were collected from residents of Kongwa district of Dodoma region, Tanzania, and examined microscopically for helminth eggs and proglottids. Of these, 4 Taenia egg positive cases were identified, and the eggs were subjected to DNA analysis. Several proglottids of Taenia solium were recovered from 1 of the 4 cases. This established that the species were T. solium (n=1) and T. saginata (n=3). One further T. solium specimen was found among 128 fecal samples collected from Mbulu district in Arusha, and this had an intact strobila with the scolex. Phylegenetic analysis of the mtDNA cox1 gene sequences of these 5 isolates showed that T. saginata was basal to the T. solium clade. The mitochondrial cox1 gene sequences of 3 of these Tanzanian isolates showed 99% similarity to T. saginata, and the other 2 isolates showed 100% similarity to T. solium. The present study has shown that Taenia tapeworms are endemic in Kongwa district of Tanzania, as well as in a Previously identified Mbulu district. Both T. solium isolates were found to have an "African/Latin American" genotype (cox1).

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