Parasites, Hosts and Diseases

  • 제49권4호
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  • Pages.381-384
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  • 2011
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Identification of a Trichinella Isolate from a Naturally Infected Pig in Tibet, China

  • Li, Ling Zhao (Department of Parasitology, Medical College, Zhengzhou University) ;
  • Wang, Zhong Quan (Department of Parasitology, Medical College, Zhengzhou University) ;
  • Jiang, Peng (Department of Parasitology, Medical College, Zhengzhou University) ;
  • Zhang, Xi (Department of Parasitology, Medical College, Zhengzhou University) ;
  • Ren, Hui Jun (Department of Parasitology, Medical College, Zhengzhou University) ;
  • Cui, Jing (Department of Parasitology, Medical College, Zhengzhou University)
  • 투고 : 2011.08.17
  • 심사 : 2011.09.30
  • 발행 : 2011.12.15
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초록

The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial largesubunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.

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참고문헌

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피인용 문헌

  1. Comparison of three molecular detection methods for detection of Trichinella in infected pigs vol.112, pp.5, 2011, https://doi.org/10.1007/s00436-012-3267-1
  2. Innovative molecular diagnosis of T richinella species based on β‐carbonic anhydrase genomic sequence vol.9, pp.2, 2016, https://doi.org/10.1111/1751-7915.12327
  3. Surveillance and diagnosis of zoonotic foodborne parasites vol.6, pp.1, 2011, https://doi.org/10.1002/fsn3.530
  4. Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis vol.13, pp.1, 2011, https://doi.org/10.1186/s13071-020-3981-y