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Evaluation of Rheological and Functional Properties of Roasted Soybean Flour and Mixed Cereals Fermented by Bacillus sp.

고초균을 이용한 볶은 콩과 곡류 혼합 발효물의 물성 및 기능성 평가

  • Son, Se-Jin (Dept. of Food Science and Technology and The Center for Traditional Microorganism Resources (TMR), Keimyung University) ;
  • Lee, Sam-Pin (Dept. of Food Science and Technology and The Center for Traditional Microorganism Resources (TMR), Keimyung University)
  • 손세진 (계명대학교 식품가공학과, 전통 미생물자원개발 및 산업화 연구센터) ;
  • 이삼빈 (계명대학교 식품가공학과, 전통 미생물자원개발 및 산업화 연구센터)
  • Received : 2010.12.17
  • Accepted : 2011.02.07
  • Published : 2011.03.31

Abstract

Roasted soybean flour (RSF) and mixed cereals were fermented by the solid state fermentation using Bacillus subtilis HA to optimize the production of biologically active compounds. The RFS fermented with 52.7% moisture content showed higher production with protease activity of 42.6 unit/g and 10% mucilage content after fermentation for 24 hr. Tyrosine content and protease activity after 48 hr fermentation time were the highest values with 445.5 mg% and 55.1 unit/g, respectively. However, the wholesome fermented RSF (FRSF) was obtained by fermentation for 24 hr because of the production of unpleasant flavors after fermentation for 48 hr. The RSF fermented with various types and contents of cereals has no effects on tyrosine content and protease activity. However, the addition of brown rice significantly increased mucilage content, especially indicating 24.55% at the addition of 80% (w/w). For addition of barley, fibrinolytic activity was increased to 11.82 unit/g by the fortification of 60% barley. It is concluded that biologically active compounds including fibrinolytic activity and mucilage content in FRSF were dependent upon the type and content of various cereals.

본 연구에서는 콩 비린내가 감소되고 고소한 풍미가 증진된 볶은 콩에 다양한 볶은 곡류 분말을 혼합하여 Bacillus subtilis HA 고초균을 이용한 고체발효를 행하였다. 발효 원료의 수분함량 및 발효 시간에 따른 결과 볶은 콩 분말 대비 수분 1배 첨가된 초기 수분함량 52.6%에서 24시간 발효 후에 protease 활성이 42.6 unit/g으로 높았으며, 점질물 함량 역시 10.60%로 높았다. 발효 48시간째 tyrosine 함량과 protease 활성이 24시간째보다 높게 나타났으나, 발효취가 강하게 생성되어 발효 24시간이 가장 적합한 것으로 나타났다. 곡류 분말(보리, 현미) 종류에 따른 발효조건 최적화를 위해 실시한 결과 현미 첨가 시 점질물 함량이 유의적으로 증가되었고 특히 80% 첨가 시 24.55%로 대조구에 비해 약 2배 향상되었고, 보리는 60% 첨가 시 혈전용해효소 활성이 11.8 unit/g으로 대조구에 비해 약 2배 증진된 것을 확인하였다. 혈전용해효소 활성이 가장 우수했던 볶은 콩 대비 보리 60% 첨가 발효물에 청국장에서 분리한 균주 4가지를 접종하여 혈전용해효소 활성 및 비타민 $K_2$ 함량을 각각 알아 본 결과 B. subtilis HA가 11.8 unit/g, 2.36 ${\mu}g/g$으로 가장 효과적이었다.

Keywords

References

  1. Lim SB, Kang MS, Jwa MK, Song DJ, Oh YJ. 2003. Characteristics of cooked rice by adding grains and legumes.J Korean Soc Food Sci Nutr 32: 52-57. https://doi.org/10.3746/jkfn.2003.32.1.052
  2. Woo SM, Kim TY, Yeo SW, Kim SB, Jeong YJ. 2007.Properties of alcohol fermentation from barley treated with non-steam and steam. Korea J Food Preserv 14: 201-206.
  3. Seog HM, Seo MS, Kim HM, Ahn MS, Lee YT. 2002.Antioxidative activity of barley polyphenol extract (BPE)separated from pearling by-products. Korean J Food SciTechnol 34: 889-892.
  4. Oh SH, Park KB, Kim BJ. 2002. Changes in the levels of water soluble protein and free amino acids in brown rice germinated in a chitosan/glutamic acid solution. Korea J Biotechnol Bioeng 17: 515-519.
  5. Park JD, Choi BK, Kum JS, Lee HY. 2006. Physicochemical properties of brown rice flours produced under different drying and milling conditions. Korean J Food Sci Technol38: 495-500.
  6. Choi JH. 2001. Quality characteristics of the bread with sprouted brown rice flour. Korean J Soc Food Cookery Sci17: 323-328.
  7. Yoo HJ, Lee DS, Kim HB. 2004. Chungkookjang fermentation of mixture of barley, wormwood, sea tangle, and soybean.Korean J Microbiol 40: 49-53.
  8. Chang JH, Shim YY, Kim SH, Chee KM, Cha SK. 2005.Fibrinolytic and immunostimulating activities of Bacillus spp. strains isolated from Chungkuk-jang. Korean J FoodSci Technol 37: 255-260.
  9. Kil JO, Kim GN, Park IS. 1998. Production and characterization of fibrinolytic enzyme: optimal condition for production of the enzyme form bacillus sp. KP-6408 isolated form Chungkook-jang. J Korean Soc Food Sci Nutr 27:51-56.
  10. Kim JH, Lee JH, Kim HJ, Choi SY, Lee JS. 2003. Effects of barley koji and legumes on the quality and fibrinolyticactivity of Korean traditional rice wine. J Korean Soc FoodSci Nutr 32: 1066-1070. https://doi.org/10.3746/jkfn.2003.32.7.1066
  11. Choi NS, Seo SY, Kim SH. 1999. Screening of mushrooms having fibrinolytic activity. Korean J Food Sci Technol 31:553-557.
  12. Kim SH, Choi NS, Lee WY, Lee JW, Kim DH. 1998. Isolation of Bacillus strains secreting fibrinolytic enzymes from Doen-jang. Korean J Microbiol 34: 87-90.
  13. Kim YT. 1995. Characteristics of fibrinolytic enzyme produced by Bacillus sp. isolated from Chungkook-jang. PhD Dissertation. Sejong University, Seoul, Korea.
  14. Hong JY, Choue RW, Baek JY, Cho HJ, Song YB. 1999.The study of correlation between serum vitamin K concentration and bone metabolism in postmenopausal women.Korean J Nutr 21: 287-295.
  15. Jung HO, Lim SS, Jung BM. 1997. A study on the sensory and texture characteristics of bread with roasted soybeanpowder. Korean J Soc Food Sci 13: 266-271.
  16. Lee HC, Park CO, Shin DH. 1998. Lactic fermentation of steamed barley with an enzyme and a Lactobacillus. KoreanJ Food Nutr 1: 43-49.
  17. Oh SM. 2006. Optimization of production of bioactive compounds of the fermented soybean curd residue by Bacillus sp. MS Thesis. Keimyung University, Daegu, Korea.
  18. Ryu MJ. 2007. Study on mucilage (biopolymer) production and flocculating activity of the fermented soybean curd residue by Bacillus subtilis PUL-A. MS Thesis. Keimyung University, Daegu, Korea.
  19. Oh SM, Kim CS, Lee SP. 2006. Characterization of the functional properties of soy milk cake fermented by Bacillussp. Food Sci Biotechnol 15: 704-709.
  20. Kim HJ, Lee JJ, Cheigh MJ, Choi SY. 1998. Amylase, protease, peroxidase and ascorbic acid oxidase activity of kimchiingredient. Korean J Food Sci Technol 30: 1333-1338.
  21. Choi SH, Park JS, Whang KS, Yoon MH, Choi WY. 2004.Production of microbial biopolymer, poly (${\gamma}$-glutamic acid)by Bacillus subtilis BS 62. Agric Chem Biotechnol 47:60-64.
  22. Astrup T, Mullerz S. 1952. The fibrin plate method for estimation fibrinolytic activity. Arch Biochem Biophys 40: 346-351. https://doi.org/10.1016/0003-9861(52)90121-5
  23. Lee HT, Lee MJ, Lee SS. 2009. Physicochemical characteristics of soybean pastes containing sword bean. J FoodEngineering Progress 13: 176-182.
  24. Choi BD, Lee SK, Yun SE, Joo HK. 1998. Effect of mugwort extract on the quality and the changes of chemical compositionsof the Cheongkukjang prepared with frozen soybean.Agric Chem Biol 41: 510-515.
  25. Lee JH, Bai DH. 2004. A thermostable protease produced from Bacillus sp. DF 218. Korea J Food Sci Thechnol 34:105-110.
  26. Oh SM, Kim CS, Lee SP. 2006. Functional properties of soybean curd residue fermented by Bacillus sp. J KoreanSoc Food Sci Nutr 35: 115-120. https://doi.org/10.3746/jkfn.2006.35.1.115
  27. Beleia A, Varriano-Marstion E, Hoseney RC. 1980. Characterization of starch from pearl millets. Cereal Chem 57:300-303.
  28. Kim MH, Kang WW, Lee NH, Kwon DJ, Kwon OJ, ChungYS, Hwang YH, Choi UK. 2007. Changes in quality characteristics of Cheonggukjang made with germinated soybean.Korean J Food Technol 39: 676-680.
  29. Sohn BH, Song YJ, Oh KH. 2008. Fibrinolytic activity and characterization of Bacillus licheniformis HK-12 isolatedfrom Chungkook-jang. Korean J Biotechnol Bioeng 23:251-256.
  30. Han KH, Lee JC, Lee GS, Kim JH, Lee JS. 2002. Manufacture and physiological functionality of Korean traditional liquor by using purple-fleshed sweet potato. Korean J Food SciTechnol 34: 673-677.
  31. Kil JO, Kim GN, Park IS. 1998. Production and characterization of fibrinolytic enzyme: Optimal condition for production of the enzyme from Bacillus sp. KP-6408 isolatedfrom Chungkook-jang. J Korean Soc Food Sci Nutr 27:51-56.
  32. Son KH, Choue RW. 2006. A study comparison of dietary quality and vitamin k intake of vegetarians with carnivores.Korean J Nutr 39: 529-538.

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