The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides

  • Lian, Sen (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Jonson, Miranda Gilda (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Cho, Won-Kyong (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Choi, Hong-Soo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Je, Yeon-Ho (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University)
  • 투고 : 2010.11.05
  • 심사 : 2011.01.23
  • 발행 : 2011.03.01
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초록

Rice stripe virus (RSV) is one of serious epidemic pathogens for rice species grown in many Asian countries. Therefore, it is necessary to produce a diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary, the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation, protein purification, and western blot analysis.

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참고문헌

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피인용 문헌

  1. Interaction study of rice stripe virus proteins reveals a region of the nucleocapsid protein (NP) required for NP self-interaction and nuclear localization vol.183, 2014, https://doi.org/10.1016/j.virusres.2014.01.011
  2. Identification of residues or motif(s) of the rice stripe virus NS3 protein required for self-interaction and for silencing suppressor activity vol.235, 2017, https://doi.org/10.1016/j.virusres.2017.03.022
  3. Rice grassy stunt virus nonstructural protein p5 serves as a viral suppressor of RNA silencing and interacts with nonstructural protein p3 vol.160, pp.11, 2015, https://doi.org/10.1007/s00705-015-2560-6
  4. Prevalence of rice stripe virus can be altered by temperature and the virus-mediated development of insect vector, Laodelphax striatellus , in Korea vol.20, pp.4, 2017, https://doi.org/10.1016/j.aspen.2017.08.012
  5. RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses vol.27, pp.3, 2011, https://doi.org/10.5423/PPJ.2011.27.3.291
  6. Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing vol.27, pp.4, 2011, https://doi.org/10.5423/PPJ.2011.27.4.310
  7. Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast–Chloroplast Proteomics vol.20, pp.2, 2019, https://doi.org/10.3390/ijms20020253