Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Immunological Change of the Lymph Node and Lymph Follicles, Stimulated LPS in the Popliteal Lymph Node of the Early Postnatal Mice

Lipopolysaccharide 항원에 노출된 발생초기의 림프절내 B 및 T 림프구의 면역학적 변화

  • Received : 2010.12.20
  • Accepted : 2011.02.10
  • Published : 2011.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was designed to clarify the morphometrical change of lymph node, deep cortex and lymph follicles in draining lymph nodes of young mice in response to local injection of lipopolysaccharide(LPS). 1. In the group stimulated with LPS, aged 0 day and 3 days, the number of lymph follicles were not significantly different from those of control group. 2. In the group two to four weeks after injection with LPS, aged five days and one week, the number of lymph follicles were significantly increased from those of control group. 3. In the group one to four weeks after injection with LPS, aged 0 day, three days, five days and one week, the area of lymph node and deep cortex increased about 1.5-3 times more than that of the control group. 4. In the group two to four weeks after injection with LPS, aged three days, five days and one week, the lymph follicles(the area: larger than 0.1 mm2) were increased from those of control group. 5. In the group two to four weeks after injection with LPS, aged five days and one week, the lymph follicles(the area: smaller than 0.01 mm2) were increased from those of control group. In view of these experimental findings, the formation of lymph follicles were induced by LPS stimulation from 5 days to one week after birth. The newley formed lymph follicles area in response to LPS may be less than $0.01mm^2$.

가슴샘 비의존성 항원이며 B 림프구의 분열원로 알려져 있는 lipopolysaccharide(LPS)를 발육초기의 C57BL/6계 생쥐에 투여한 후 림프조직의 성숙과정에 따른 면역반응의 양상을 발생학적인 측면에서 형태학적으로 규명하고자 본 실험을 시행하였다. 1. 생후 0일과 3일에 LPS를 주사한 군에서는 주사 후 시간경과에 따라서 림프소절의 총수가 대조군에 비하여 증가되지 않았다. 2. 생후 5일과 7일에 LPS를 주사한 후 2-4주가 경과되었을 때 림프소절의 총수는 대조군에 비하여 유의성있게 증가하였다. 3. 생후 0일에서부터 1주 사이에 LPS를 주사한 후 1-4주가 경과된 실험군에서는 모든 림프절과 심층피질의 면적이 대조군에 비하여 약 1.5-3배정도 커졌다. 4. 생후 3일, 5일 및 1주에 LPS를 주사한 후 2-4주가 경과된 실험군에서는 면적이 0.1 mm2 이상인 림프소절들이 대조군에 비하여 증가하였다. 5. 생후 5일과 1주에 LPS를 주사한 후 2-4주가 경과된 실험군에서는 면적이 0.01 mm2 미만인 림프소절들이 대조군에 비하여 증가하였다. 이상의 실험결과로 미루어 볼 때 LPS의 자극에 의한 새로운 림프소절의 형성능력은 생후 5일-1주에 이루어지며, 또한 새로 생성되는 림프소절들은 면적이 0.01 mm2 미만인 작은 림프소절들일 것으로 예측된다.

Keywords

References

  1. Fahy VA, Gerber HA, Morris B et al, "The function of lymph nodes in the formulation of lymph", Monogr Allergy, 16:82-99,1980.
  2. Sainte-Marie G, Peng FS, Belisle C, "Overall architecture and pattern of lymph flow in the rat lymph node", Am J Anat, 164:275-309, 1982. https://doi.org/10.1002/aja.1001640402
  3. Weissman IL , "In developmental aspects of antibody formation and structure" Academia Plague, 1:55, 1970.
  4. Markgraf R, von Gaudecker B, Müller-Hermelink K , "The development of the human lymph node", Cell Tissue Res, 225:387-413,1982. https://doi.org/10.1007/BF00214691
  5. Gutman GA, Weissman IL, "Lymphoid tissue architecture. Experimental analysis of the origin and distribution of T-cells and B-cells", Immunol, 23:465- 479, 1972.
  6. Ham W, Cormak DH, "Histology. 8th ed, Philadelphia and Toronto", Lippincott, pp. 345,1979.
  7. Goldschneider I, McGregor DD, "Anatomical distribution of T and B lymphocytes in the rat. Development of lymphocyte-specific antisera", J Exp Med 138:1443-1465,1973. https://doi.org/10.1084/jem.138.6.1443
  8. Belisle C, Saint-Marie G, "Tridimensional study of the deep cortex of the rat lymph node. II : Relation of deep cortex units to afferent lymphatic vessels", Anat Rec, 199:61-72,1981. https://doi.org/10.1002/ar.1091990107
  9. Hoshi H, Kamiya K, Takemoto R, Aijima H, "Formation of lymph follicles and germinal centers in draining lymph nodes after local injection of phytohemagglutinin and lipoplysaccharide in mice", Arch Histol Jap,47:505-518, 1984. https://doi.org/10.1679/aohc.47.505
  10. Klaus GGB, Humphrey JH, Kunkl A, Dongworth DW," The follicular dendritic cell : Its role in antigen presentation in the generation of immunological memory" Immunol Rev, 53:3-18,1980. https://doi.org/10.1111/j.1600-065X.1980.tb01038.x
  11. Nossal GJV, Ada GL, Austinl CM, "Antigens in immunity. IV.: Cellular localization of 125I-and 131I-labelled flagella in lymph nodes", Aust J Exp Bio Med Sci, 42:311-330, 1964. https://doi.org/10.1038/icb.1964.32
  12. Kim Chang Hee, "Bacto aduuvant 면역보강제 자극 후 오금림프절내 림프소절의 변화에 관한 형태계측학적 연구" Kyung Hee University dissertation, 2006.
  13. Na Jong Du다, "Concanavalin A의 자극에 의한 생쥐오금림프절내 림프소절과 속겉질의 변화에 관한 형태계측학적 연구" Kyung Hee University dissertation, 2000
  14. Lee Jun beom, "Alteration of the clusterin expression in the spleen and the lymph node in the mouse", Korea university dissertation 2008.
  15. Kang Hyung Jeong, "Analysis of lymph node metastasis in early gastric cancer" Pusan National University, 2010.
  16. Park Jun Seok, "Comparison between in vivo and ex vivo injection fo blue dye in sentinel lymph node mapping for colon cancer"Chung-ang university dissertation, 2008.
  17. Villena A, Zapata A, Rivera-Pomar JM, Barrutia MG, Fonfua J, "Structure of the non-lymphoid cells during the postnatal development of the rat lymph nodes" Cell Tissue Res, 229:219-232, 1983.
  18. Clark SI, Schneider GB, "Role of the adrenal cortex in maturation of the lymphoid system and immunological competence; the effects of aminoglutethimide in sucking mice", Am J Anat, 137:231-256, 1973. https://doi.org/10.1002/aja.1001370302
  19. Schwander R, Hess MW, Keller HU, Cottier H, "The Postnatal development of lymph nodes in mice, "Immunobiol, 157:425-436,1980. https://doi.org/10.1016/S0171-2985(80)80012-X
  20. Eikelenboom P, Levenbach MGE, van der Brink HR, Streefkerk JG, "Development of T and B cell areas in peripheral lymphoid organs of the rat", Anat Rec, 194:523-538, 1979. https://doi.org/10.1002/ar.1091940406
  21. Imai Y, Dobashi M, Terashima K, "Postnatal development of dendritic reticulum cells and their immune complex trapping ability", Histol Histopath, 1:19-26,1986.
  22. Friedberg SH, Weissman IL, "Lymphoid tissue architecture. II. Ontogeny of peripheral T and B cell in mice. Evidence against Peyer's patches as the site of generation of B cells", J Immunol, 113:1477-1492, 1974.
  23. Joel DD, Hess MW, Cottier H, "Magnitude and pattern of thymic lymphocyte migration in neonatal mice", J Exp Med, 135:907-923, 1972. https://doi.org/10.1084/jem.135.4.907
  24. Van Rees EP, Dijkstra CD, van Rooijen N, "The early postnatal development of the primary immune response in rat popliteal lymph node, stimulated with thymus-independent type-1 and type-2 antigens" ,Cell Tissue Res,250:695-699, 1987. https://doi.org/10.1007/BF00218965
  25. Horie K, Hoshi H, "Induction of lymph follicle formation with several mitogens and adjuvants in the mouse popliteal lymph node", Histology & Histopathology, 4:17-25, 1989.
  26. Ahn HK, Hoshi H, Horie K, Nagata H, "Sites of lymph follicle formation in the draining popliteal lymph nodes of mice locally injected with antigenic and mitogenic substances" Histology & Histopathology, 5:493-504, 1990.
  27. Parrott DMV, De Sausa MAB, East J, "Thymusdependent areas in the lymphoid organs of neonatally thymectomized mice", J Exp Med, 123:191-203, 1966. https://doi.org/10.1084/jem.123.1.191
  28. Yang UK, Ahn HK, "Lipopolysaccharide activation by antigenic stimulation changes and the formation of lymph follicles on the immunohistochemical study", Journal of Anatomy, 23 : 50- 61,1990.