Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Role of Cannabinoid on Neuronal Differentiation of P19 Cells

  • Ju, Hyun-Hee (Department of Life Science, Kyonggi University) ;
  • Ghil, Sung-Ho (Department of Life Science, Kyonggi University)
  • ;
  • 길성호 (경기대학교 생명과학과)
  • Received : 2011.08.03
  • Accepted : 2011.09.24
  • Published : 2011.09.30
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Abstract

P19 cells are pluripotent embryonal carcinoma cells and can be differentiated into neuronal cell type by treatment with retinoic acid (RA) and aggregation culture. Cannabinoids are the active components of Cannabis sativa and they have diverse pharmacologic activities, such as pain control, anti-inflammatory effects, neuro-protection effects and tumor regression. Cannabinoids also involved in neuronal proliferation, migration, differentiation and survival in developing brain. Here, we studied the role of cannabinoids on neuronal differentiation of P19 cells. Treatment with cannabinoids increased the neuronal differentiation induced by RA and also promoted transcriptional activity of neurogenin 1, key transcription factor for neuronal differentiation of P19 cells. These results suggest that the cannabinoids can accelerate neuronal differentiation of P19 cells.

Keywords

References

  1. Aguado T, Monory K, Palazuelos J, Stella N, Cravatt B, Lutz B, Marsicano G, Kokaia Z, Guzman M, Galve-Roperh I. The endocannabinoid system drives neural progenitor proliferation. FASEB J. 2005. 19: 1704-1706. https://doi.org/10.1096/fj.05-3995fje
  2. Bain G, Ray WJ, Yao M, Gottlieb DI. From embryonal carcinoma cells to neurons: the P19 pathway. Bioessays. 1994. 16: 343 -348. https://doi.org/10.1002/bies.950160509
  3. Bisogno T, Ligresti A, Di Marzo V. The endocannabinoid signalling system: biochemical aspects. Pharmacol Biochem Behav. 2005. 81: 224-238. https://doi.org/10.1016/j.pbb.2005.01.027
  4. Cho JH, Kwon IS, Kim S, Ghil SH, Tsai MJ, Kim YS, Lee YD, Suh-Kim H. Overexpression of BETA2/NeuroD induces neurite outgrowth in F11 neuroblastoma cells. J Neurochem. 2001. 77: 103-109. https://doi.org/10.1046/j.1471-4159.2001.t01-1-00230.x
  5. Ghil S, Choi JM, Kim SS, Lee YD, Liao Y, Birnbaumer L, Suh-Kim H. Compartmentalization of protein kinase A signaling by the heterotrimeric G protein Go. Proc Natl Acad Sci U S A. 2006. 103: 19158-19163. https://doi.org/10.1073/pnas.0609392103
  6. Ghil SH, Kim BJ, Lee YD, Suh-Kim H. Neurite outgrowth induced by cyclic AMP can be modulated by the alpha subunit of Go. J Neurochem. 2000. 74: 151-158.
  7. Hohmann AG, Suplita RL 2nd. Endocannabinoid mechanisms of pain modulation. AAPS J. 2006. 17: E693-708.
  8. Hu DL, Chen FK, Liu YQ, Sheng YH, Yang R, Kong XQ, Cao KJ, Gu HT, Qian LM. GATA-4 promotes the differentiation of P19 cells into cardiac myocytes. Int J Mol Med. 2010. 26: 365-372.
  9. Jiang W, Zhang Y, Xiao L, Van Cleemput J, Ji SP, Bai G, Zhang X. Cannabinoids promote embryonic and adult hippocampus neurogenesis and produce anxiolytic- and antidepressant-like effects. J Clin Invest. 2005. 115: 3104-3116. https://doi.org/10.1172/JCI25509
  10. Jin K, Xie L, Kim SH, Parmentier-Batteur S, Sun Y, Mao XO, Childs J, Greenberg DA. Defective adult neurogenesis in CB1 cannabinoid receptor knockout mice. Mol Pharmacol. 2004. 66: 204-208. https://doi.org/10.1124/mol.66.2.204
  11. Jordan JD, He JC, Eungdamrong NJ, Gomes I, Ali W, Nguyen T, Bivona TG, Philips MR, Devi LA, Iyengar R. Cannabinoid receptor-induced neurite outgrowth is mediated by Rap1 activation through G(alpha)o/i-triggered proteasomal degradation of Rap1GAPII. J Biol Chem. 2005. 280: 11413-11421. https://doi.org/10.1074/jbc.M411521200
  12. Kim D-W, Kim G-J, Kim H-J, Ghil S-H. Fluorescence-based Assay System for Endocannabinoid Degradation Enzyme, Fatty Acid Amide Hydrolase J. Exp. Biomed. Sci. 2010. 16: 279-285.
  13. Kim S, Yoon YS, Kim JW, Jung M, Kim SU, Lee YD, Suh-Kim H. Neurogenin1 is sufficient to induce neuronal differentiation of embryonal carcinoma P19 cells in the absence of retinoic acid. Cell Mol Neurobiol. 2004. 24: 343-356. https://doi.org/10.1023/B:CEMN.0000022767.74774.38
  14. Kim SH, Kim S, Ghil SH. Rit contributes to neurite outgrowth triggered by the alpha subunit of Go. Neuroreport. 2008. 19: 521-525. https://doi.org/10.1097/WNR.0b013e3282f9e473
  15. Lee JE, Hollenberg SM, Snider L, Turner DL, Lipnick N, Weintraub H. Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein. Science. 1995. 268: 836-844. https://doi.org/10.1126/science.7754368
  16. Ma Q, Sommer L, Cserjesi P, Anderson DJ. Mash1 and neurogenin 1 expression patterns define complementary domains of neuroepithelium in the developing CNS and are correlated with regions expressing notch ligands. J Neurosci. 1997. 17: 3644-3652.
  17. Matsuda LA, Lolait SJ, Brownstein MJ, Young AC, Bonner TI. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 1990. 346: 561-564. https://doi.org/10.1038/346561a0
  18. McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 1982. 299: 165-167. https://doi.org/10.1038/299165a0
  19. Murre C, McCaw PS, Vaessin H, Caudy M, Jan LY, Jan YN, Cabrera CV, Buskin JN, Hauschka SD, Lassar AB. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell 1989. 58: 537-544. https://doi.org/10.1016/0092-8674(89)90434-0
  20. Palazuelos J, Aguado T, Egia A, Mechoulam R, Guzman M, Galve-Roperh I. Non-psychoactive CB2 cannabinoid agonists stimulate neural progenitor proliferation. FASEB J. 2006. 20: 2405-2407. https://doi.org/10.1096/fj.06-6164fje
  21. Pan B, Hillard CJ, Liu QS. D2 dopamine receptor activation facilitates endocannabinoid-mediated long-term synaptic depression of GABAergic synaptic transmission in midbrain dopamine neurons via cAMP-protein kinase A signaling. J Neurosci. 2008. 28: 14018-14030. https://doi.org/10.1523/JNEUROSCI.4035-08.2008
  22. Rueda D, Navarro B, Martinez-Serrano A, Guzman M, Galve- Roperh I. The endocannabinoid anandamide inhibits neuronal progenitor cell differentiation through attenuation of the Rap1/B-Raf/ERK pathway. J Biol Chem. 2002 277: 46645 -46650. https://doi.org/10.1074/jbc.M206590200
  23. Rujano MA, Pina P, Servitja JM, Ahumada AM, Picatoste F, Farres J, Sabria J. Retinoic acid-induced differentiation into astrocytes and glutamatergic neurons is associated with expression of functional and activable phospholipase D. Biochem Biophys Res Commun. 2004. 316: 387-392. https://doi.org/10.1016/j.bbrc.2004.02.057
  24. Schlicker E, Kathmann. Modulation of transmitter release via presynaptic cannabinoid receptors. Trends Pharmacol Sci. 2001. 22: 565-572. https://doi.org/10.1016/S0165-6147(00)01805-8
  25. Teets BW, Soprano KJ, Soprano DR. Role of SF-1 and DAX-1 during differentiation of P19 cells by retinoic acid. J Cell Physiol. 2011. [Epub ahead of print]