Clinical and Experimental Reproductive Medicine

  • 제38권1호
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  • Pages.18-23
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  • 2011
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Expression of peroxiredoxin I regulated by gonadotropins in the rat ovary

  • Lee, Yu-Il (Department of Obstetrics and Gynecology, Chonnam National University Medical School) ;
  • Kang, Woo-Dae (Department of Obstetrics and Gynecology, Chonnam National University Medical School) ;
  • Kim, Mi-Young (Department of Obstetrics and Gynecology, Chonnam National University Medical School) ;
  • Cho, Moon-Kyoung (Department of Obstetrics and Gynecology, Chonnam National University Medical School) ;
  • Chun, Sang-Young (Hormone Research Center, Chonnam National University Medical School)
  • 투고 : 2010.09.02
  • 심사 : 2010.10.26
  • 발행 : 2011.03.02
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초록

Objective: Peroxiredoxins (Prxs) play an important role in regulating cellular differentiation and proliferation in several types of mammalian cells. This report examined the expression of Prx isotype I in the rat ovary after hormone treatment. Methods: Immature rats were injected with 10 IU of pregnant mare's serum gonadotropin (PMSG) to induce the growth of multiple preovulatory follicles and 10 IU of human chorionic gonadotropin (hCG) to induce ovulation. Immature rats were also treated with diethylstilbestrol (DES), an estrogen analogue, to induce the growth of multiple immature follicles. Northern blot analysis was performed to detect gene expression. Cell-type specific localization of Prx I mRNA were detected by in situ hybridization analysis. Results: During follicle development, ovarian Prx I gene expression was detected in 3-day-old rats and had increased in 21-day-old rats. The levels of Prx I mRNA slightly declined one to two days following treatment with DES. A gradual increase in Prx I gene expression was observed in ovaries obtained from PMSG-treated immature rats. Furthermore, hCG treatment of PMSG-primed rats resulted in a gradual stimulation of Prx I mRNA levels by 24 hours (2.1-fold increase) following treatment, which remained high until 72 hours following treatment. In situ hybridization analysis revealed the expression of the Prx I gene in the granulosa cells of PMSG-primed ovaries and in the corpora lutea of ovaries stimulated with hCG for 72 hours. Conclusion: These results demonstrate the gonadotropin and granulosa cell-specific stimulation of Prx I gene expression, suggesting its role as a local regulator of follicle development.

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참고문헌

  1. de Haan JB, Bladier C, Lotfi-Miri M, Taylor J, Hutchinson P, Crack PJ, et al. Fibroblasts derived from Gpx1 knockout mice display senescent-like features and are susceptible to $H_2O_2$-mediated cell death. Free Radic Biol Med 2004;36:53-64.
  2. Fujii J, Ikeda Y. Advances in our understanding of peroxiredoxin, a multifunctional, mammalian redox protein. Redox Rep 2002;7: 123-30. https://doi.org/10.1179/135100002125000352
  3. Das KC. Thioredoxin system in premature and newborn biology. Antioxid Redox Signal 2004;6:177-84. https://doi.org/10.1089/152308604771978480
  4. Dietz KJ, Horling F, König J, Baier M. The function of the chloroplast 2-cysteine peroxiredoxin in peroxide detoxification and its regulation. J Exp Bot 2002;53:1321-9. https://doi.org/10.1093/jexbot/53.372.1321
  5. Rouhier N, Jacquot JP. Plant peroxiredoxins: alternative hydroperoxide scavenging enzymes. Photosynth Res 2002;74:259-68. https://doi.org/10.1023/A:1021218932260
  6. Brehelin C, Meyer EH, de Souris JP, Bonnard G, Meyer Y. Resemblance and dissemblance of Arabidopsis type II peroxiredoxins: similar sequences for divergent gene expression, protein localization, and activity. Plant Physiol 2003;132:2045-57. https://doi.org/10.1104/pp.103.022533
  7. Ishii T, Kawane T, Taketani S, Bannai S. Inhibition of the thiol-specific antioxidant activity of rat liver MSP23 protein by hemin. Biochem Biophys Res Commun 1995;216:970-5. https://doi.org/10.1006/bbrc.1995.2715
  8. Kang SW, Chae HZ, Seo MS, Kim K, Baines IC, Rhee SG. Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor alpha. J Biol Chem 1998;273:6297-302. https://doi.org/10.1074/jbc.273.11.6297
  9. Kim H, Lee TH, Park ES, Suh JM, Park SJ, Chung HK, et al. Role of peroxiredoxins in regulating intracellular hydrogen peroxide and hydrogen peroxide-induced apoptosis in thyroid cells. J Biol Chem 2000;275:18266-70. https://doi.org/10.1074/jbc.275.24.18266
  10. Bryk R, Griffin P, Nathan C. Peroxynitrite reductase activity of bacterial peroxiredoxins. Nature 2000;407:211-5. https://doi.org/10.1038/35025109
  11. Seo MS, Kang SW, Kim K, Baines IC, Lee TH, Rhee SG. Identification of a new type of mammalian peroxiredoxin that forms an intramolecular disulfide as a reaction intermediate. J Biol Chem 2000;275:20346-54. https://doi.org/10.1074/jbc.M001943200
  12. Zhou Y, Kok KH, Chun AC, Wong CM, Wu HW, Lin MC, et al. Mouse peroxiredoxin V is a thioredoxin peroxidase that inhibits p53-induced apoptosis. Biochem Biophys Res Commun 2000;268:921-7. https://doi.org/10.1006/bbrc.2000.2231
  13. Ishii T, Yamada M, Sato H, Matsue M, Taketani S, Nakayama K, et al. Cloning and characterization of a 23-kDa stress-induced mouse peritoneal macrophage protein. J Biol Chem 1993;268: 18633-6.
  14. Siow RC, Ishii T, Sato H, Taketani S, Leake DS, Sweiry JH, et al. Induction of the antioxidant stress proteins heme oxygenase-1 and MSP23 by stress agents and oxidised LDL in cultured vascular smooth muscle cells. FEBS Lett 1995;368:239-42. https://doi.org/10.1016/0014-5793(95)00650-X
  15. Kovacic N, Parlow AF. Alterations in serum FSH-LH ratios in relation to the estrous cycle, pseudopregnancy, and gonadectomy in the mouse. Endocrinology 1972;91:910-5. https://doi.org/10.1210/endo-91-4-910
  16. Lee J, Park HJ, Choi HS, Kwon HB, Arimura A, Lee BJ, et al. Gonadotropin stimulation of pituitary adenylate cyclase-activating polypeptide (PACAP) messenger ribonucleic acid in the rat ovary and the role of PACAP as a follicle survival factor. Endocrinology 1999;140:818-26. https://doi.org/10.1210/en.140.2.818
  17. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition 2002;18:872-9. https://doi.org/10.1016/S0899-9007(02)00916-4
  18. Cao Z, Lindsay JG, Isaacs NW. Mitochondrial peroxiredoxins. Subcell Biochem 2007;44:295-315.
  19. Klaunig JE, Xu Y, Isenberg JS, Bachowski S, Kolaja KL, Jiang J, et al. The role of oxidative stress in chemical carcinogenesis. Environ Health Perspect 1998;106 Suppl 1:289-95. https://doi.org/10.1289/ehp.98106s1289
  20. Lee AC, Fenster BE, Ito H, Takeda K, Bae NS, Hirai T, et al. Ras proteins induce senescence by altering the intracellular levels of reactive oxygen species. J Biol Chem 1999;274:7936-40. https://doi.org/10.1074/jbc.274.12.7936
  21. Immenschuh S, Baumgart-Vogt E. Peroxiredoxins, oxidative stress, and cell proliferation. Antioxid Redox Signal 2005;7:768-77. https://doi.org/10.1089/ars.2005.7.768
  22. Rhee SG, Chae HZ, Kim K. Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling. Free Radic Biol Med 2005;38:1543-52. https://doi.org/10.1016/j.freeradbiomed.2005.02.026
  23. Wood ZA, Poole LB, Karplus PA. Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling. Science 2003;300: 650-3. https://doi.org/10.1126/science.1080405
  24. Rhee SG. Cell signaling. H2O2, a necessary evil for cell signaling. Science 2006;312:1882-3. https://doi.org/10.1126/science.1130481
  25. Jin DY, Chae HZ, Rhee SG, Jeang KT. Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation. J Biol Chem 1997;272:30952-61. https://doi.org/10.1074/jbc.272.49.30952
  26. Sawada M, Carlson JC. Rapid plasma membrane changes in superoxide radical formation, fluidity, and phospholipase A2 activity in the corpus luteum of the rat during induction of luteolysis. Endocrinology 1991;128:2992-8. https://doi.org/10.1210/endo-128-6-2992
  27. Kirsch TM, Friedman AC, Vogel RL, Flickinger GL. Macrophages in corpora lutea of mice: characterization and effects on steroid secretion. Biol Reprod 1981;25:629-38. https://doi.org/10.1095/biolreprod25.3.629
  28. Gatzuli E, Aten RF, Behrman HR. Inhibition of gonadotropin action and progesterone synthesis by xanthine oxidase in rat luteal cells. Endocrinology 1991;128:2253-8. https://doi.org/10.1210/endo-128-5-2253
  29. Sawada M, Carlson JC. Studies on the mechanism controlling generation of superoxide radical in luteinized rat ovaries during regression. Endocrinology 1994;135:1645-50. https://doi.org/10.1210/en.135.4.1645
  30. Kumar TR, Wang Y, Lu N, Matzuk MM. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 1997;15:201-4. https://doi.org/10.1038/ng0297-201
  31. Uilenbroek JT, Richards JS. Ovarian follicular development during the rat estrous cycle: gonadotropin receptors and follicular responsiveness. Biol Reprod 1979;20:1159-65. https://doi.org/10.1095/biolreprod20.5.1159
  32. Zhou J, Kumar TR, Matzuk MM, Bondy C. Insulin-like growth factor I regulates gonadotropin responsiveness in the murine ovary. Mol Endocrinol 1997;11:1924-33. https://doi.org/10.1210/me.11.13.1924
  33. Adashi EY, Resnick CE, Svoboda ME, Van Wyk JJ. Somatomedin-C enhances induction of luteinizing hormone receptors by folliclestimulating hormone in cultured rat granulosa cells. Endocrinology 1985;116:2369-75. https://doi.org/10.1210/endo-116-6-2369
  34. Findlay JK. An update on the roles of inhibin, activin, and follistatin as local regulators of folliculogenesis. Biol Reprod 1993; 48:15-23. https://doi.org/10.1095/biolreprod48.1.15
  35. Zlotkin T, Farkash Y, Orly J. Cell-specific expression of immunoreactive cholesterol side-chain cleavage cytochrome P-450 during follicular development in the rat ovary. Endocrinology 1986;119: 2809-20. https://doi.org/10.1210/endo-119-6-2809
  36. Ronen-Fuhrmann T, Timberg R, King SR, Hales KH, Hales DB, Stocco DM, et al. Spatio-temporal expression patterns of steroidogenic acute regulatory protein (StAR) during follicular development in the rat ovary. Endocrinology 1998;139:303-15. https://doi.org/10.1210/en.139.1.303

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  3. The Relevance of Mammalian Peroxiredoxins to the Gametogenesis, Embryogenesis, and Pregnancy Outcomes vol.24, pp.6, 2011, https://doi.org/10.1177/1933719116667217
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