Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2

  • Han, Seung-Hee (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Kim, Kyoung-Hwa (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Han, Jung-Seok (Department of Prosthodontics, Seoul National University School of Dentistry) ;
  • Koo, Ki-Tae (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Kim, Tae-Il (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Seol, Yang-Jo (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Lee, Yong-Moo (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Ku, Young (Department of Periodontology, Seoul National University School of Dentistry) ;
  • Rhyu, In-Chul (Department of Periodontology, Seoul National University School of Dentistry)
  • Received : 2011.07.02
  • Accepted : 2011.08.28
  • Published : 2011.10.31
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Abstract

Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC3T3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC3T3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2 (BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2), osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2 than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin, Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium.

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