한국환경생태학회지 (Korean Journal of Environment and Ecology)

  • 제25권5호
  • /
  • Pages.658-665
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  • 2011
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  • 1229-3857(pISSN)
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  • 2288-131X(eISSN)
한국환경생태학회 (Korean Society of Environment and Ecology)
권호 표지

제주도 한라산에 서식하는 도입종 야생멧돼지에 대한 분자유전학적 분석

A Molecular Genetic Analysis of the Introduced Wild Boar Species (Sus scrofa coreanus) on Mount Halla, Jeju Island, Korea

  • 한상현 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 오장근 (제주특별자치도 환경자원연구원) ;
  • 조인철 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 고문석 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 김태욱 (제주대학교 생물학과) ;
  • 장민호 (제주대학교 생물학과) ;
  • 김병수 (제주대학교 생물학과) ;
  • 박수곤 (제주대학교 과학교육과) ;
  • 오홍식 (제주대학교 과학교육과)
  • Han, Sang-Hyun (Subtropical Animal Experiment Station, National Institute of Animal Science, R.D.A.) ;
  • Oh, Jang-Geun (Institute of Environmental Resource Research, Jeju) ;
  • Cho, In-Cheol (Subtropical Animal Experiment Station, National Institute of Animal Science, R.D.A.) ;
  • Ko, Moon-Suck (Subtropical Animal Experiment Station, National Institute of Animal Science, R.D.A.) ;
  • Kim, Tae-Wook (Department of Biology, Jeju National University) ;
  • Chang, Min-Ho (Department of Biology, Jeju National University) ;
  • Kim, Byoung-Soo (Department of Biology, Jeju National University) ;
  • Park, Su-Gon (Department of Science Education, Jeju National University) ;
  • Oh, Hong-Shik (Department of Science Education, Jeju National University)
  • 투고 : 2011.01.03
  • 심사 : 2011.10.01
  • 발행 : 2011.10.31
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초록

제주도에서는 절멸된 것으로 간주되었던 멧돼지들이 최근 한라산 인근지역에서 발견되었다. 본 연구는 분자유전학적 실험기법을 바탕으로 한라산 멧돼지들이 가축돼지들과 이종교배된 것들인지를 조사하였다. 또한 동일 종내에서의 유전적 유연관계와 분자 성판별을 시험하였다. 가축돼지 품종들(Landrace, Large White, Berkshire, Hampshire, Duroc)과의 교배여부는 핵 DNA와 미토콘드리아 DNA에서 4 종류의 분자 표지인자(MC1R, KIT, 조절영역, ND2)를 적용하여 시험하였다. 야생멧돼지 집단의 모든 개체들이 동일한 mtDNA 조절영역 서열을 나타내었고, 그 서열들은 중국 동북부 재래돼지들과 동일하였으나 기존에 보고된 한반도 멧돼지의 서열들과는 다른 것으로 확인되었다. 이상의 연구결과는 한라산 멧돼지집단이 중국 재래돼지 품종들과 근연이면서, 기존에 연구되지 않았던 유전적 계통에서 유래한 것으로 사료된다. 분자 성판별 결과 수컷에 비해 암컷이 2 배 이상으로 확인되어, 한라산 야생멧돼지 집단이 팽창하고 있으며, 조절하지 않으면 집단 규모는 극적으로 증가할 것이다.

An wild boar species which has been known as an extinct species on Jeju Island, was recently observed in the surrounding areas of Mount Halla. Based on the molecular techniques, this study examines whether they are crossbred with domesticated pig breeds. Intraspecific genetic relationships with other wild boar populations and molecular sexing were examined as well. Total of four molecular markers on mitochondrial DNA(control region and ND2) and nuclear DNA(MC1R and KIT) were applied to test crossbreeding between with domesticated pig breeds, such as Landrace, Large White, Berkshire, Hampshire, and Duroc. All individuals of wild boar population had identical mtDNA control region(CR) sequences. In addition, the sequences were the same as those of some native pig breeds which are distributed in Northeast China, but different from those previously reported from the Korean Peninsula up to date. These results suggest that this population may have originated from a genetic lineage had been not previously studied and genetically related to Chinese native pig breeds. Molecular sexing results show that there are twice as many females as male. Thus the population is under expansion and its size will dynamically increase if not controlled.

키워드

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