생명과학회지 (Journal of Life Science)

  • 제21권12호
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  • Pages.1761-1771
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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생약복합조성물(HemoHIM)의 자외선 조사로 억제된 랑게르한스 세포의 항원제시기능 방호효과

Inhibitory Effects of a New Herbal Composition (HemoHIM) on UVB-Induced Suppression of Langerhans Cell's Accessory Cell Function

  • 김종진 (순천대학교 생명산업과학대학 생물학과) ;
  • 조성기 (한국원자력연구원 정읍 방사선과학연구소 방사선생명공학연구부) ;
  • 정우희 (한국원자력연구원 정읍 방사선과학연구소 방사선생명공학연구부) ;
  • 박혜란 (한국원자력연구원 정읍 방사선과학연구소 방사선생명공학연구부) ;
  • 이성태 (순천대학교 생명산업과학대학 생물학과)
  • Kim, Jong-Jin (Department of Biology, College of Life Science and Natural Resources, Sunchon National University) ;
  • Jo, Sung-Kee (Radiation Research Division for Bio-Technology, Advanced Radiation Technology Institute, Jeongeup campus of Korea Atomic Energy Research Institute) ;
  • Jung, U-Hee (Radiation Research Division for Bio-Technology, Advanced Radiation Technology Institute, Jeongeup campus of Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Radiation Research Division for Bio-Technology, Advanced Radiation Technology Institute, Jeongeup campus of Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
  • 투고 : 2011.11.04
  • 심사 : 2011.12.20
  • 발행 : 2011.12.31
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초록

방사선 조사에 대한 방호 효과와 방사선 치료에 의한 부작용 경감 효과를 가지는 새로운 생약복합조성물(HemoHIM)의 자외선 조사에 의한 피부 면역계 방호 효과에 대해 알아보았다. IL-4와 GM-CSF로 분화시킨 수 지상세포에 자외선을 직접 조사하여 항원전달기능의 감소를 유발하는 자외선 조사 조건을 확립하고, HemoHIM을 자외선 조사 전 후에 처리하여 자외선에 의해 감소한 수지상세포의 항원전달기능이 회복되는 것을 확인하였다. 그리고 접촉성 과민반응 모델을 이용하여 피부의 Langerhans 세포의 항원전달기능의 감소를 유발하는 자외선 조사 조건을 확립하고, Langerhans 세포의 항원전달기능의 감소를 유발하고 HemoHIM을 각각 복강 또는 경구로 투여하는 방법으로 처리하였을 때, 억제된 Langerhans 세포의 항원전달 기능을 회복시키는 효과가 있다는 것을 확인하였다. 이상의 결과로 HemoHIM은 자외선 조사로 저하된 피부 면역 기능을 회복시키는 효과가 있다는 것을 증명하였고, 피부 면역 기능을 개선하는 새로운 자외선 차단제 개발을 위한 소재로 사용할 가능성을 제시하였다.

In the previous results, we developed an effective products to apply as functional foods for overcome of radiation damage and reduction of side effects in radiotherapy. To verify the prevention of UVB-induced immunosuppression of immune cell function by HemoHIM, we studied on the mechanism of the skin immune function for the protection in UVB irradiation. In studies presented here, we showed that HemoHIM can prevent UVB-induced impairment of skin immune cell function by in vitro and in vivo assay. Exposure of freshly cultured murine dendritic cells (DCs) with IL-4/GM-CSF to UVB irradiation resulted in impairment of accessory function. This suppression could be prevented by addition of HemoHIM before or after to the cultures of UVB-irradiated DCs. We also tested the effects of HemoHIM on the suppression of contact hypersensitivity (CHS) treated oral or intraperitoneal administration. This UVB-suppressed CHS was prevented by administration of HemoHIM to UVB-irradiated mice. These results suggest that HemoHIM may prevent UVB-induced immune suppression in the skin.

키워드

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피인용 문헌

  1. Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4 vol.16, pp.1, 2016, https://doi.org/10.1186/s12906-016-1045-9