Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Bacillus subtilis CK-2 Hydrolysing Various Organic Materials

다양한 유기물을 분해하는 Bacillus subtilis CK-2의 분리

  • Kim, Chul-Ho (Department of Phamaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Lee, Sang-Hyup (Department of Phamaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김철호 (경남과학기술대학교 제약공학과) ;
  • 이상협 (경남과학기술대학교 제약공학과)
  • Received : 2011.09.29
  • Accepted : 2011.12.06
  • Published : 2011.12.31
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Abstract

A bacterium hydrolysing various organic materials including cellulose, protein, starch and lipid was isolated. The isolate was identified as Bacillus subtilis, and named Bacillus subtilis CK-2 in this paper. This bacterium showed optimal growth at $40\sim45^{\circ}C$, pH 6~9, and 0~3% of NaCl. B. subtilis CK-2 seemed to synthesis highly active autolysin. The hydrolytic enzymes produced by B. subtilis CK-2 were primary enzymes because extracellular enzyme activities varied similarly to the growth curve. The hydrolytic enzymes seemed to be stable at basic pH conditions. From these results, B. subtilis CK-2 was found to bea useful bacterial agent for composting, or for use in feed-production waste in agriculture, fishery, forest materials, livestock farming, and food.

섬유소를 비롯한 단백질, 지질, 녹말을 분해할 수 있는 세균을 된장으로부터 분리하여 동정한 결과 Bacillus subtilis로 분류되었으며, Bacillus subtilis CK-2로 명명하였다. 분리균주는 $40\sim45^{\circ}C$의 비교적 넓은 온도 범위와 pH 6~9의 넓은 pH 범위, 그리고 NaCl 0~3% 범위에서 잘 자랐으며, 높은 자가분해효소 활성을 갖는 것을 알 수 있었다. B. subtilis CK-2가 분비하는 가수분해효소들은 대부분 세균의 생장과 거의 비례적으로 세포외 활성을 나타내는 1차 대사산물로 확인되었다. 이상의 결과로부터 B. subtilis CK-2는 농수임산물 폐기물이나 음식물 폐기물의 퇴비화, 사료 생산 등에 유용하게 이용될 수 있을 것으로 생각한다.

Keywords

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