Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Screening and Identification of Soy Curd-Producing Lactic Acid Bacteria

두유 커드를 생산하는 김치 유래 젖산균의 동정

  • Kim, Ro-Ui (Department of Food Science & Technology, Pusan National University) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Yu, Sun-Nyoung (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Kim, Kwang-Youn (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Seong, Jong-Hwan (Department of Food Science & Technology, Pusan National University) ;
  • Lee, Young-Guen (Department of Food Science & Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science & Technology, Pusan National University) ;
  • Kim, Dong-Seob (Department of Food Science & Technology, Pusan National University)
  • 김로의 (부산대학교 식품공학과) ;
  • 안순철 (부산대학교 의학전문대학원 미생물학 교실) ;
  • 유선녕 (부산대학교 의학전문대학원 미생물학 교실) ;
  • 김광연 (부산대학교 의학전문대학원 미생물학 교실) ;
  • 성종환 (부산대학교 식품공학과) ;
  • 이영근 (부산대학교 식품공학과) ;
  • 김한수 (부산대학교 식품공학과) ;
  • 김동섭 (부산대학교 식품공학과)
  • Received : 2010.10.15
  • Accepted : 2010.12.01
  • Published : 2011.02.28
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Abstract

The purpose of this study was to isolate soy curd forming bacterial strains. Soy curd forming bacteria were isolated from Kimchi, a traditional Korean vegetable food that is fermented using lactic acid bacteria. Among 196 bacterial strains, ten isolates (strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5) formed firm soy curd. The isolated bacterial strains were identified by molecular biological and biochemical analyses. The genomic DNAs extracted from the isolated bacterial strains were used as a template for PCR amplification of 16S rDNA region. By comparing the results of the 16s rDNA sequences with GenBank data, the isolated strains were identified as Leuconostoc mesenteroides group and Lactobacillus sakei group. The phylogenetic position of soy curd forming strains and their related taxa were investigated using neighbor-joining method. L. mesenteroides group was further identified as L. mesenteroides subsp. dextranicum based on biochemical properties. L. sakei group was named Lactobacillus sp., because it showed a variety of biochemical properties.

본 연구의 목적은 두유 curd를 형성하는 미생물을 분리하는 것이다. 두유 curd를 형성하는 미생물은 채소를 젖산균으로 발효시킨 전통적인 한국의 음식, 김치로부터 분리하였다. 분리 균주 196개 중 10개의 균주(strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5)가 단단한 두유 curd를 형성하였고 분자생물학적 생화학적 분석법에 의해 동정되었다. 분리균주로부터 추출한 genomic DNA는 16S rDNA 지역의 PCR 증폭을 위한 주형으로 사용하였다. GenBank 데이터로 16S rDNA 염기서열을 비교한 결과, 분리 균주들은 Leuconostoc mesenteroides group과 Lactobacillus sakei group으로 동정되었다. 두유 curd를 형성하는 균주들의 계통 발생학적 위치와 분류군은 neighbor-joining 방법을 이용하여 확인하였다. 또한, L. mesenteroides group은 생화학적 특성에 의해 L. mesenteroides subsp. dextranicum으로 동정되었다. 하지만 L. sakei group은 생화학적 특성 비교시 다양성을 보여 Lactobacillus sp.로 명명하였다.

Keywords

References

  1. Aneja, R. P., B. N. Mathur, R. C. Chandan, and S. K. Banerjee. 2002. Cultured/ Fermented products. In: Traditional Indian Milk Products Handbook on Process Technology Modernization for Professionals, Entrepreneurs and Scientists, pp. 158-182, In Gupta, P. R. (ed.), New Delhi, India:Dairy India Publication.
  2. Baek, Y. J., C. S. Huh, and J. Y. Lee. 1998. Utilization of fermented milk and it's health promotion. Korean J. Dairy Sci. Technol. 17, 58-71.
  3. Chien, H. L., H. Y. Huang, and C. C. Chou. 2006. Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria. Food Microbiol. 23, 772-778. https://doi.org/10.1016/j.fm.2006.01.002
  4. Choi, Y. B., J. G. Woo, and W. S. Noh. 1999. Hydrolysis of ${\beta}$-glycosidic bonds of isoflavone conjugates in the lactic acid fermentation of soy milk. Korean J. Food Sci. Technol. 31, 189-195.
  5. Hendrich, S., W. Lee, X. Xu, J. H. Wang, and P. A. Murphy, 1994. Defining food component as new nutrient. J. Nutr. 124, 1789-1792.
  6. Karleskind, D., I. Laye, E. Halpin, and C. V. Morr. 1991. Improving acid production in soy based yogurt by adding cheese whey proteins and mineral salts. J. Food Sci. 56, 999-1001. https://doi.org/10.1111/j.1365-2621.1991.tb14626.x
  7. Kim, T. W., S. H. Jung, J. Y. Lee, S. K. Choi, S. H. Park, J. S. Jo, and H. Y. Kim. 2003. Identification of lactic acid bacteria in Kimchi using SDS-PAGE profiles of whole cell proteins. J. Micrbiol. Biotechnol. 13, 119-124.
  8. Lee, H. P., L. Gourley, S. W. Duffy, J. Esteve, J. Lee, and N. E. Day. 1991. Dietary effects on breast-cancer risk in Singapore. Lancet 337, 197-200.
  9. Lim, S. J., Y. T. Kho, and J. C. Yoo. 1984. Manufacture of yogurt from soy protein concentrate. Food Sci. Biotechnol. 16, 143-148.
  10. MacGregor, G., A. J. Smith, B. Thakker, and J. Kinsella. 2002. Yoghurt biotheraphy contraindicated in immunosuppressed patients? Postgrad. Med. J. 78, 366-367. https://doi.org/10.1136/pmj.78.920.366
  11. Mann, G. V. and A. Spoerry. 1974. Studies of a surfactant and cholesteremia in the massi. Am. J. Clin. Nutr. 27, 464-469.
  12. Messina, M. 1995. Modern applications for an ancient bean: soybeans and the prevention and treatment of chronic disease. J. Nutr. 125, 567-569.
  13. Messina, M., V. Persky, K. D. R. Setchell, and S. Branes. 1994. Soy intake and cancer risk, A review of the in vitro and in vivo data. Nutr. Cancer 21, 113-131. https://doi.org/10.1080/01635589409514310
  14. Mun, S. A. and Y. T. Ko. 1986. Keeping quality of yogurt beverage prepared from soy protein isolate. Food Sci. Biotechnol. 18, 124-129.
  15. Schneeman, B. O. 1986. Physical and chemical properties, methods of analysis, and physiological effects. Food Technol. 40, 104-110.
  16. Park, K. B. and S. H. Oh. 2007. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresource Technol. 98, 1675-1679. https://doi.org/10.1016/j.biortech.2006.06.006
  17. Peterson, G. 1995. Evolution of the biochemical targets of genistein in tumor cells. J. Nutr. 125, 784-789.
  18. Reddy, G. V., K. M. Shahani, and M. R. Banerjee. 1973. Inhibitory effect of yogurt on Ehrlich ascites tumor cell proliferation. J. Natl. Cancer Inst. 50, 815-817.
  19. Wang, Y. C., R. C. Yu, and C. C. Chou. 2006. Antioxidant activities of soymilk fermented with lactic acid bacteria and bifidobacteria. Food Microbiol. 23, 128-135. https://doi.org/10.1016/j.fm.2005.01.020
  20. Yoon, S. Y. and S. J. Hwang. 2005. A survey on the level of recognizing Kimchi among housewives in Seoul Area. Korean J. Food Culture 20, 405-415.

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